Recombinant Mouse Desmoglein-3 (Dsg3), partial

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Code CSB-EP007205MOe1
Size $472
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Product Details

Purity
Greater than 85% as determined by SDS-PAGE.
Target Names
Dsg3
Uniprot No.
Research Area
Others
Alternative Names
Dsg3Desmoglein-3; 130 kDa pemphigus vulgaris antigen homolog
Species
Mus musculus (Mouse)
Source
E.coli
Expression Region
50-617aa
Target Protein Sequence
EWVKFAKPCREREDNSRRNPIAKITSDFQKNQKITYRISGVGIDQPPFGIFVVDPNNGDINITAIVDREETPSFLITCRALNALGQDVERPLILTVKILDVNDNPPIFSQTIFKGEIEENSASNSLVMILNATDADEPNHMNSKIAFKIVSQEPAGMSMFLISRNTGEVRTLTSSLDREQISSYHLVVSGADNDGTGLSTQCECSIKIKDVNDNFPVLRESQYSARIEENTLNAELLRFQVTDWDEEYTDNWLAVYFFTSGNEGNWFEIETDPRTNEGILKVVKALDYEQVQSMQFSIAVRNKAEFHQSVISQYRVQSTPVTIQVIDVREGISFRPPSKTFTVQRGVSTNKLVGYILGTYQATDEDTGKAASSVRYVLGRNDGGLLVIDSKTAQIKFVKNIDRDSTFIVNKTISAEVLAIDENTGKTSTGTIYVEVPSFNENCPSVVLEKKDICTSSPSVTLSVRTLDRGKYTGPYTVSLEEQPLKLPVMWTITTLNATSALLQAQQQVSPGVYNVPVIVKDNQDGLCDTPESLTLTVCQCDDRSMCRAPIPSREPNTYGESSWRLGP
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight
63.2 kDa
Protein Length
Partial
Tag Info
Tag-Free
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.

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Target Background

Function
Component of intercellular desmosome junctions. Involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion.
Gene References into Functions
  1. flotillin has a role in desmosomal adhesion and Pemphigus vulgaris-like localisation of desmoglein-3 in human keratinocytes PMID: 27346727
  2. Desmoglein 3 deficiency in mice leads to a phenotype characterized by cyclic alopecia in addition to the dramatic skin and mucocutaneous acantholysis observed in pemphigus patients. PMID: 25542773
  3. Dsg3, via p38 MAPK, regulates the spatial distribution of the keratin filament network and stabilizes cell adhesion. PMID: 24782306
  4. Squamous metaplasia after pulmonary epithelial injury may play a crucial role in redirecting the skin-specific autoimmune reaction to the lungs in paraneoplastic pemphigus (PNP). PMID: 23729442
  5. Dsg3 deficiency results in perturbation of the desmosomal network at the transcriptional, translational, and interaction level, kinase activation, proteinase-mediated degradation, and hyper-adhesion. (Review) PMID: 23368972
  6. Thus, Dsg3 does not display a clear function as a tumor suppressor in these mouse skin cancer models PMID: 23185521
  7. IgG and IgM antibodies against desmoglein 3 have a pathogenic role in blister formation in pemphigus vulgaris PMID: 21718682
  8. the adult passive transfer mouse model is ideally suited for detailed studies of Dsg3 antibody-mediated signaling in adult skin. PMID: 21956125
  9. These data indicate a contribution of Dsg depletion to pemphigus vulgaris pathogenesis dependent on Ca(2+)-induced differentiation. PMID: 21864491
  10. Aire has an important role in Dsg3 expression as well as in selection of T cells that help B cells to produce anti-Dsg3 IgG in thymus. PMID: 21048786
  11. Loss of tolerance against Dsg3 in both B and T cells is important for the development of autoimmune state of pemphigus vulgaris. PMID: 11857336
  12. Patchy hair loss in pemphigus vulgaris model mice as in Dsg3(-/-) mice. Ultrastructure of telogen hair follicles same as PV model mice and Dsg3(-/-) mice. Anti-Dsg3 autoantibodies interfere with cell-cell adhesion of keratinocytes in PV model mice. PMID: 12373337
  13. perturbation of desmoglein 3 found in the Dsg3bal-Pas mice resulted in disadhesion of keratinocytes manifested with blistering phenotype. PMID: 12485423
  14. anti-Dsg3 IgG antibodies can directly access Dsg3 present in desmosomes in vivo and cause the subsequent desmosome separation that leads to blister formation in pemphigus vulgaris PMID: 15140217
  15. In pemphigus vulgaris antibodies were dominantly raised against middle to C-terminal extracellular domains of mouse Dsg3 where amino acid sequences are less conserved among desmoglein isoforms. Those antibodies may also be involved in blister formation. PMID: 15265525
  16. importance of the companion layer, and particularly the Dsg1 and Dsg3 in this layer, in anchoring the anagen hair to the follicle. PMID: 15482466
  17. Northern Blot analysis of wild-type and Plucked (pk) mutant mice indicates that expression of both desmoglein 1 (Dsg1) and desmoglein 3 (Dsg3) is up-regulated in the skin of mutant pk mice PMID: 15629430
  18. skin-specific Dsg3 serves as an autoantigen in chronic skin inflammatory diseases accompanied by mast cell degranulation, including both murine systemic lupus erythematosus and other autoinflammatory diseases PMID: 16492738
  19. Reduction of Dsg3 might be relevant to blister formation in pemphigus vulgaris. PMID: 17431647
  20. distinct T cell responses could be switched after active immunization combined with different adjuvants, resulting in distinct anti-Dsg3 antibody isotypes with different pathogenic activities in Pemphigus disease development PMID: 18634826
  21. Subcellular localization of desmosomal components is different between desmoglein3 knockout mice and pemphigus vulgaris model mice. PMID: 19525093

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Subcellular Location
Cell membrane; Single-pass type I membrane protein. Cell junction, desmosome.
Tissue Specificity
Expressed in epidermis.
Database Links
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