Recombinant Poecilobdella viridis Hirudin (Active)

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Code CSB-AP000241PNX
Size $354
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Product Details

Purity
>96% as determined by SDS-PAGE and HPLC.
Endotoxin
Less than 1.0 EU/μg as determined by LAL method.
Activity
The biological activity is determined by chromogenic assay, 1 unit is defined as the amount of Hirudin that neutralizes 1 unit of the WHO preparation 89/588 of thrombin. The specific activity is no less than 14,000 ATU/mg protein.
Target Names
N/A
Uniprot No.
Research Area
Neuroscience?
Alternative Names
Hirudin
Species
Poecilobdella viridis (Indian freshwater leech)
Source
Yeast
Expression Region
1-63aa
Complete Sequence
VVYTDCTESG QNLCLCEGSN VCGQGNKCIL GSDGEKNQCV TGEGTPGPQS HNDGDFEEPE EYL
Mol. Weight
6.7 kDa
Protein Length
Full Length
Tag Info
Tag-Free
Form
Liquid or Lyophilized powder
Buffer
Lyophilized from a 0.2 ?m filtered solution of 20 mM PBS, pH 7.0, containing 2 % mannitol
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
5-10 business days
Datasheet & COA
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Hirudin is a potent thrombin-specific protease inhibitor. It forms a stable non-covalent complex with alpha-thrombin, thereby abolishing its ability to cleave fibrinogen.
Subcellular Location
Secreted.
Protein Families
Protease inhibitor I14 (hirudin) family
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