Product Details

Purity

≥95% as determined by SDS-PAGE.

Endotoxin

Less than 1.0 EU/μg as determined by LAL method.

Activity

Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 0.5 ng/ml, corresponding to a specific activity of >2.0x10⁶ IU/mg.

Target Names

Ctf1

Uniprot No.

Q63086

Research Area

Immunology

Alternative Names

Ctf1Cardiotrophin-1; CT-1

Species

Rattus norvegicus (Rat)

Source

E.coli

Expression Region

1-203aa

Complete Sequence

MSQREGSLED HQTDSSFSFL PHLEAKIRQT HNLARLLTKY ADQLLEEYVQ QQGEPFGLPG FSPPRLPLAG LSGPAPSHAG LPVSERLRQD AAALSALPAL LDAVRRRQAE LNPRAPRLLR SLEDAARQVR ALGAAVETVL AALGAAARGP VPEPVATSAL FTSNSAAGVF SAKVLGLHVC GLYGEWVSRT EGDLGQLVPG GVA

Mol. Weight

21.4 kDa

Protein Length

Full Length

Tag Info

Tag-Free

Form

Lyophilized powder

Buffer

Lyophilized from a 0.2 µm filtered 20 mM PB, pH 7.4, 150mM NaCl

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

5-10 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

Cardiotrophin-1 signals through the gp130/LIFR complex to activate JAK/STAT and MAPK pathways, making it a critical tool for dissecting IL-6 family cytokine biology in cardiac hypertrophy and immune cell regulation. This tag-free recombinant rat CT-1 covers the full-length mature sequence (aa 1–203) and demonstrates an ED50 below 0.5 ng/ml in TF-1 cell proliferation assays, corresponding to a specific activity exceeding 2.0 × 10⁶ IU/mg—potency that supports use as a reference standard in cytokine detection assays and provides reliable dose-response curves in cell-based functional studies. The endotoxin level of less than 1.0 EU/μg satisfies the criteria typical for LPS-sensitive applications such as primary immune cell differentiation assays, STAT3 phosphorylation studies, and in vivo rat models of inflammation or cardiac stress, where even trace endotoxin contamination would confound cytokine-specific readouts. Purity exceeding 95% by SDS-PAGE further supports its suitability as a coating antigen or positive control in ELISA-based antibody validation workflows.

Target Background

Function

Induces cardiac myocyte hypertrophy in vitro. Binds to and activates the ILST/gp130 receptor.

Gene References into Functions

Cardiotrophin-1 modulates glucose transport in cardiomyocytes positively or negatively depending on its concentration. PMID: 23277190
Cardiomyocytes treated with CT-1 present high spontaneous Ca(2+) release during diastole, a mechanism linked to arrhythmogenicity in the pathologic heart. PMID: 22787135
Cardiotrophin 1 is involved in cardiac, vascular, and renal fibrosis and dysfunction. PMID: 22733458
Data suggest a key role for CT-1 in cardiac remodeling induced by aldosterone independent of changes in blood pressure levels. PMID: 21926338
CT-1 induces VSMCs proliferation, hypertrophy and extracellular matrix production, and is upregulated in hypertension and by aldosterone. CT-1 may represent a new target of vascular wall remodeling in hypertension PMID: 20216087
Cardiotrophin-1 promotes the outgrowth of embryonic cranial motor neurons in isolated stage E12 hindbrain explants. PMID: 11932952
CT1 has a role in hypertrophy of rat cardiomyocytes as measured by an increase in cell area and enhanced protein: DNA ratio PMID: 12027405
Ctf1 has a possible role in post-MI wound healing PMID: 14674704
hypertrophic responses to cardiotrophin-1 is mitogen activated protein kinase kinase 5-mitogen-activated protein kinase 7 pathway PMID: 15623435
Motoneurons retrogradely transport from the tongue radiolabeled GDNF, BDNF, and cardiotrophin-1; cardiotrophin-1 follows a strict degradation pathway after retrograde transport to the soma. PMID: 15659589
CT-1 can affect the expression of GATA4 mRNA and the binding activity of cardiac myocytes PMID: 19327894
CT-1 signals through p38, ERK, and Akt in a parallel manner to activate NF-kappa B and that NF-kappa B is required for CT-1 to mediate its full cytoprotective effects in cardiac myocytes. PMID: 11448959

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Subcellular Location

Secreted.

Protein Families

IL-6 superfamily

Tissue Specificity

Expressed in the ventricle and atrium of adult rats. Also detected in the lung, kidney, liver, skeletal muscle, stomach and urinary bladder. Not detected in brain, colon, testis, spleen or thymus. Overexpressed in the ventricles in the case of hypertensio

Database Links

KEGG: rno:29201

STRING: 10116.ENSRNOP00000025646

UniGene: Rn.10253

Code	CSB-AP000211RA
Abbreviation	Recombinant Rat Ctf1 protein (Active)
MSDS	MSDS Datasheet
Size	$354
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Recombinant Rat Cardiotrophin-1 protein (Ctf1) (Active)

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Target Background

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