Purity

≥ 95% as determined by SDS-PAGE.

Endotoxin

≤200 EU/mg by the LAL method

Activity

Measured in a cell proliferation assay using NIH3T3 mouse embryonic fibroblast cells. The ED50 for this effect is ≤2.0 ng/mL.

Target Names

bFGF/FGF-2

Uniprot No.

XP_030252529.1 A0A671VAT8

Alternative Names

Fibroblast Growth Factor 2; FGF-2; Basic Fibroblast Growth Factor; bFGF; Heparin-Binding Growth Factor 2; HBGF-2; FGF2; FGFB

Species

Sparus aurata (Gilthead sea bream)

Source

E.coli

Expression Region

1-155aa

Target Protein Sequence

MATGEITTLPSTPEDGGSGGFPPGSFKEPKRLYCKNGGFFLRIKSDGGVDGIREKNDPHIKLQLQATSVGEVVIKGVCANRYLAMNRDGRLFGARRATDECHFLERLESNNYNTYRSRKYPNMYVALKRTGQYKSGSKTGPGQKAILFLPMSAKC

Mol. Weight

17.1 kDa

Protein Length

Full Length

Tag Info

Tag free

Form

Lyophilized
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

Lyophilized from a 0.2 μm filtered solution containing 10 mM PB, 250 mMNaCl, 5% mannitol and 0.01% Tween 80, pH7.4

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

FGF-2 orchestrates cell proliferation, survival, and angiogenesis across vertebrate species, and this full-length recombinant protein from gilthead sea bream (aa 1–155) delivers potent mitogenic activity with an ED50 ≤2.0 ng/mL in NIH3T3 fibroblast proliferation assays, demonstrating receptor-binding competence suitable for comparative studies of FGF signaling in aquaculture and evolutionary biology. Produced in E. coli without tags, the protein retains native sequence architecture and supports cell proliferation assays, receptor-ligand binding experiments by ELISA or surface plasmon resonance, and angiogenesis models where FGF-2 pathway activation is under investigation. Purity exceeding 95% by SDS-PAGE and endotoxin levels ≤200 EU/mg align with standards expected in mammalian cell culture and functional assays requiring low pyrogen contamination. This reagent provides a basis for exploring FGF-2 function in teleost physiology, cross-species receptor specificity studies, and aquatic organism regenerative biology.

Code	CSB-EP6199FJX-WD
Abbreviation	Recombinant Sparus aurata FGF-2 protein (Active)
MSDS	MSDS Datasheet
Size	$200
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Recombinant Sparus aurata Fibroblast growth factor (fgf2) (Active)

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