A2ML1 Antibody

Datasheet

Code	CSB-PA001005LA01HU
Size	US$166
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Image	Western Blot Positive WB detected in: A549 whole cell lysate All lanes: A2ML1 antibody at 3.2µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 162, 106 kDa Observed band size: 162 kDa Immunohistochemistry of paraffin-embedded human lung cancer using CSB-PA001005LA01HU at dilution of 1:100
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) A2ML1 Polyclonal antibody

Uniprot No.

A8K2U0

Target Names

A2ML1

Alternative Names

A2ML1 antibody; CPAMD9Alpha-2-macroglobulin-like protein 1 antibody; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 9 antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Alpha-2-macroglobulin-like protein 1 protein (625-751AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The A2ML1 Antibody (Product code: CSB-PA001005LA01HU) is Non-conjugated. For A2ML1 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA001005LB01HU	A2ML1 Antibody, HRP conjugated	ELISA
FITC	CSB-PA001005LC01HU	A2ML1 Antibody, FITC conjugated
Biotin	CSB-PA001005LD01HU	A2ML1 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:20-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

The A2ML1 antibody is generated from the rabbit immunized with a partial recombinant protein corresponding to 625-751 amino acids of human A2ML1 protein. It exhibits the unconjugated IgG isoform. Its specificity for human A2ML1 protein has been validated in ELISA, IHC, and WB applications. The purity of this A2ML1 antibody is greater than 95% using protein G purified.

Its target protein A2ML1 mainly inhibits protease activity, contributing to protecting tissues from degradation by enzymes. A2ML1 also plays a role in several biological processes, including immune response, wound healing, and tissue regeneration. Mutations in the A2ML1 gene have been associated with several developmental disorders, including Noonan syndrome-like disorder with loose anagen hair.

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Target Background

Function

Is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase. Displays inhibitory activity against chymotrypsin, papain, thermolysin, subtilisin A and, to a lesser extent, elastase but not trypsin. May play an important role during desquamation by inhibiting extracellular proteases.

Gene References into Functions

The functional assessment supported the pathogenicity of the RAF1 and RIT1 variants of unknown significance (VUSs), while the significance of two VUSs in A2ML1 remained unclear. PMID: 29402968
The indigenous Filipino population has a ~50% prevalence of otitis media. A2ML1 genotype is the primary risk factor for otitis media and main determinant of disease progression, although age, the middle ear microbiome, and social clusters might modulate the effect of the A2ML1 genotype. PMID: 27484237
A2ML1-related otitis media susceptibility may be mediated by changes in the middle ear microbiome. PMID: 27799062
Studies support a role for alpha-2-macroglobulin-like 1 protein (A2ML1) in the pathophysiology of otitis media. PMID: 26121085
our results provide evidence that mutations in A2ML1 are a cause of Noonan-like syndrome, with a variable phenotype ranging from severe to very mild. PMID: 24939586
a new class of target antigens in a paraneoplastic autoimmune multiorgan syndrome PMID: 20805888
alpha2ML1 is the first alpha2macroglobulin family member detected in the epidermis, where it may play an important role during desquamation by inhibiting extracellular proteases. PMID: 16298998
alpha2ML1 binding to the low density lipoprotein receptor-related protein 1 (LRP1) reveals a new role for LRP1 in the human epidermis PMID: 18648652

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Subcellular Location

Secreted.

Protein Families

Protease inhibitor I39 (alpha-2-macroglobulin) family

Tissue Specificity

In the epidermis, expressed predominantly in the granular layer at the apical edge of keratinocytes (at protein level). Also detected in placenta, testis and thymus but not in epithelia of kidney, lung, small intestine or colon.

Database Links

HGNC: 23336

OMIM: 610627

KEGG: hsa:144568

STRING: 9606.ENSP00000299698

UniGene: Hs.620532

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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