Your Good Partner in Biology Research
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Your Good Partner in Biology Research
| Code | CSB-PA001005LA01HU |
| See More Details |  |
| Size |
US$299Purchase it in Cusabio online store (Shop online in the US. Customers outside the US, please order from local distributors.) |
| Uniprot No. | A8K2U0 |
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| Protocols | ELISA Protocol Western Blotting(WB) Protocol Immunohistochemistry (IHC) Protocol |
| Immunogen | Recombinant Human Alpha-2-macroglobulin-like protein 1 protein (625-751AA) |
| Raised in | Rabbit |
| Species Reactivity | Human |
| Tested Applications | ELISA, WB, IHC; Recommended dilution: WB:1:500-1:5000, IHC:1:20-1:200 |
| Relevance | Is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase (By similarity). Displays inhibitory activity against chymotrypsin, papain, thermolysin, subtilisin A and, to a lesser extent, elastase but not trypsin. May play an important role during desquamation by inhibiting extracellular proteases. |
| Form | Liquid |
| Conjugate | Non-conjugated |
| Storage Buffer | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Purification Method | >95%, Protein G purified |
| Isotype | IgG |
| Clonality | Polyclonal |
| Alias | Alpha-2-macroglobulin-like protein 1 (C3 and PZP-like alpha-2-macroglobulin domain-containing protein 9), A2ML1, CPAMD9 |
| Immunogen Species | Human |
| Research Area | Immunology |
| Target Names | A2ML1 |
| Storage | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
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| Function | Is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase (By similarity). Displays inhibitory activity against chymotrypsin, papain, thermolysin, subtilisin A and, to a lesser extent, elastase but not trypsin. May play an important role during desquamation by inhibiting extracellular proteases. |
| Subcellular Location | Secreted |
| Protein Families | Protease inhibitor I39 (alpha-2-macroglobulin) family |
| Tissue Specificity | In the epidermis, expressed predominantly in the granular layer at the apical edge of keratinocytes (at protein level). Also detected in placenta, testis and thymus but not in epithelia of kidney, lung, small intestine or colon. |
| Database Links |
HGNC: 23336 OMIM: 610627 KEGG: hsa:144568 STRING: 9606.ENSP00000299698 UniGene: Hs.620532 |
A2ML1 Antibodies for Homo sapiens (Human)
| Code | Product Name | Species Reactivity | Application |
|---|---|---|---|
| CSB-PA001005LB01HU | A2ML1 Antibody, HRP conjugated | Human | ELISA |
| CSB-PA001005LC01HU | A2ML1 Antibody, FITC conjugated | Human | ELISA |
| CSB-PA001005LD01HU | A2ML1 Antibody, Biotin conjugated | Human | ELISA |
A2ML1 Proteins for Homo sapiens (Human)
| Code | Product Name | Source |
|---|---|---|
| CSB-YP001005HU CSB-EP001005HU CSB-BP001005HU CSB-MP001005HU |
Recombinant Human Alpha-2-macroglobulin-like protein 1(A2ML1) ,partial | Yeast E.coli Baculovirus Mammalian cell |
2-hydroxyisobutyryl-HIST1H2BC (K12) Antibody
Applications: ELISA, WB, ICC, IF
Species Reactivity: Human
2-hydroxyisobutyryl-HIST1H4A (K5) Antibody
Applications: ELISA, WB, ICC, IF
Species Reactivity: Human
