ABHD2 Antibody

Code CSB-PA001094LA01HU
Size US$166
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  • Western blot
    All lanes: ABHD2 antibody at 10µg/ml
    Lane 1: Mouse heart tissue
    Lane 2: Rat gonadal tissue
    Secondary
    Goat polyclonal to rabbit IgG at 1/10000 dilution
    Predicted band size: 49 kDa
    Observed band size: 49 kDa

  • Immunohistochemistry of paraffin-embedded human gastric cancer using CSB-PA001094LA01HU at dilution of 1:100

  • Immunohistochemistry of paraffin-embedded human placenta tissue using CSB-PA001094LA01HU at dilution of 1:100

  • Immunofluorescent analysis of 293T cells using CSB-PA001094LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

  • Immunohistochemistry of paraffin-embedded human spleen tissue using CSB-PA001094LA01HU at dilution of 1:100

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Product Details

Full Product Name
Rabbit anti-Homo sapiens (Human) ABHD2 Polyclonal antibody
Uniprot No.
Target Names
ABHD2
Alternative Names
ABHD2; LABH2; Monoacylglycerol lipase ABHD2; 2-arachidonoylglycerol hydrolase; Abhydrolase domain-containing protein 2; Acetylesterase; Lung alpha/beta hydrolase 2; Progesterone-sensitive lipase; Protein PHPS1-2
Raised in
Rabbit
Species Reactivity
Human, Mouse, Rat
Immunogen
Recombinant Human Monoacylglycerol lipase ABHD2 protein (31-320AA)
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated

The ABHD2 Antibody (Product code: CSB-PA001094LA01HU) is Non-conjugated. For ABHD2 Antibody with conjugates, please check the following table.

Available Conjugates
Conjugate Product Code Product Name Application
HRP CSB-PA001094LB01HU ABHD2 Antibody, HRP conjugated ELISA
FITC CSB-PA001094LC01HU ABHD2 Antibody, FITC conjugated
Biotin CSB-PA001094LD01HU ABHD2 Antibody, Biotin conjugated ELISA
Clonality
Polyclonal
Isotype
IgG
Purification Method
>95%, Protein G purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Form
Liquid
Tested Applications
ELISA, WB, IHC, IF
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:5000
IHC 1:20-1:200
IF 1:50-1:200
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Background

Function
Progesterone-dependent acylglycerol lipase that catalyzes hydrolysis of endocannabinoid arachidonoylglycerol (AG) from cell membrane. Acts as a progesterone receptor: progesterone-binding activates the acylglycerol lipase activity, mediating degradation of 1-arachidonoylglycerol (1AG) and 2-arachidonoylglycerol (2AG) to glycerol and arachidonic acid (AA). Also displays an ester hydrolase activity against acetyl ester, butanoate ester and hexadecanoate ester. Plays a key role in sperm capacitation in response to progesterone by mediating degradation of 2AG, an inhibitor of the sperm calcium channel CatSper, leading to calcium influx via CatSper and sperm activation. May also play a role in smooth muscle cells migration.
Gene References into Functions
  1. Suppression of ABHD2 in OVCA420 cells increased phosphorylated p38 and ERK, platinum resistance, and side population cells (p<0.01, respectively). Conversely, overexpression of ABHD2 decreased resistance to anoikis (p<0.05) and the amount of phosphorylated p38 and ERK in OVCA420 and SKOV3 cells. PMID: 27323405
  2. A mutant ABHD2 developed with alanine in place of Ser(207). PMID: 27247428
  3. These results show that joint tests of main effects and gene-gene interaction reveal associations at some novel loci that were missed when considering main effects alone. PMID: 28813576
  4. ABHD2 gene single nucleotide polymorphisms, such as rs12442260 contribute to chronic obstructive pulmonary disease susceptibility in the Chinese Han population. PMID: 25880496
  5. ABHD2 is highly expressed in spermatozoa, binds progesterone, and acts as a progesterone-dependent lipid hydrolase by depleting the endocannabinoid 2-arachidonoylglycerol (2AG) from plasma membrane. PMID: 26989199
  6. Patients with selective immunoglobulin (Ig)A deficiency have a highly significant increase of homozygousity of HS1.2 allele *1, with an increase of 2.6-fold; the HS1.2 polymorphism influences Ig seric production, but not IgG switch. PMID: 20007591
  7. Our results showed that the ABHD2 was expressed in atherosclerotic lesions, and that the ABHD2 expression was significantly higher in the patients with UA than with SA. PMID: 17980156

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Subcellular Location
Cell projection, cilium, flagellum membrane; Single-pass type II membrane protein. Cell membrane; Single-pass type II membrane protein.
Protein Families
AB hydrolase superfamily, AB hydrolase 4 family
Tissue Specificity
Present in sperm (at protein level).
Database Links

HGNC: 18717

OMIM: 612196

KEGG: hsa:11057

STRING: 9606.ENSP00000268129

UniGene: Hs.122337

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