ADAM23 Antibody

Datasheet

Code	CSB-PA001285LA01HU
Size	US$166
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Image	Western Blot Positive WB detected in: Hela whole cell lysate All lanes: ADAM23 antibody at 3.2µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 92, 93 kDa Observed band size: 92 kDa IHC image of CSB-PA001285LA01HU diluted at 1:400 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. IHC image of CSB-PA001285LA01HU diluted at 1:400 and staining in paraffin-embedded human pancreatic cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunofluorescence staining of 293 cells with CSB-PA001285LA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) ADAM23 Polyclonal antibody

Uniprot No.

O75077

Target Names

ADAM23

Alternative Names

ADAM23; MDC3; Disintegrin and metalloproteinase domain-containing protein 23; ADAM 23; Metalloproteinase-like, disintegrin-like, and cysteine-rich protein 3; MDC-3

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Disintegrin and metalloproteinase domain-containing protein 23 protein (498-647AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The ADAM23 Antibody (Product code: CSB-PA001285LA01HU) is Non-conjugated. For ADAM23 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA001285LB01HU	ADAM23 Antibody, HRP conjugated	ELISA
FITC	CSB-PA001285LC01HU	ADAM23 Antibody, FITC conjugated
Biotin	CSB-PA001285LD01HU	ADAM23 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:200-1:500
IF	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Customer Reviews and Q&A

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Target Background

Function

May play a role in cell-cell and cell-matrix interactions. This is a non-catalytic metalloprotease-like protein.

Gene References into Functions

The relationships between the decreased methylation levels of the SNAI2 and ADAM23 genes and cancer de-differentiation and haematogenous dissemination, respectively, indicate novel functions of those genes in the invasive processes. PMID: 30189837
Our results demonstrate that ADAM23 expression is likely involved in the progression of epithelial ovarian cancer PMID: 29921495
ADAM23 regulates neuronal differentiation by triggering specific signaling pathways during human neural progenitor cells differentiation. PMID: 28828010
these results support the existence of a second mechanism, alternative to inhibition of protein secretion, by which ADLTE-causing LGI1 mutations exert their loss-of-function effect extracellularly, and suggest that interactions of LGI1 with both ADAM22 and ADAM23 play an important role in the molecular mechanisms leading to utosomal dominant lateral temporal epilepsy PMID: 27760137
Our data provide evidence that ADAM23 plays a role in suppression of cancer cell progression through interaction with aVb3 integrin, and suggest that downregulation of ADAM23 in SP cells may contribute toward providing a cancer stem cell phenotype PMID: 26800504
High ADAM23 gene methylation is associated with gastric tumor aggressiveness. PMID: 25740824
quantification of CXCL12 and ADAM23 methylation could be useful for the prediction of advanced stage of BC PMID: 25620615
Intratumoral heterogeneity of ADAM23 promotes tumor growth and metastasis through LGI4 and nitric oxide signals. PMID: 24662834
The expression level of ADAM23 is likely to be involved in the progression of non-small-cell lung carcinoma, and its downregulation is probably correlated with promoter methylation. PMID: 21429053
a SP1 binding site (-202/-190) that binds SP1 at the proximal promoter of human ADAM23 gene was indentified. PMID: 20851106
Together, these results indicate that ADAM23 down-regulation by methylation in brain tumors is a rare event and it may help explain why brain tumor metastases are rarely found elsewhere in the body. PMID: 15862898
ADAM23 is frequently silenced in gastric cancers by homozygous deletion or aberrant promoter methylation. PMID: 16103878
ADAM23 may be down-regulated by aberrant promoter hypermethylation during the progression of colorectal cancer. PMID: 19089928
PrP(C) is a novel molecular partner for ADAM23 in the nervous systems. PMID: 19477226
Functional role of ADAM23 during lung caner metastatic progression by negatively modulating alpha(v)beta(3) integrin activation. PMID: 19549921

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Subcellular Location

Cell membrane; Single-pass type I membrane protein.; [Isoform Gamma]: Secreted.

Tissue Specificity

Highly expressed in the brain and weakly expressed in the heart. In the brain, expressed prominently in the amygdala, caudate nucleus, hypothalamus, thalamus, cerebral cortex and occipital pole.

Database Links

HGNC: 202

OMIM: 603710

KEGG: hsa:8745

STRING: 9606.ENSP00000264377

UniGene: Hs.370287

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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