ANO2 Antibody

Datasheet

Code	CSB-PA885689LA01HU
Size	US$166
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Image	Western Blot Positive WB detected in: Hela whole cell lysate, U87 whole cell lysate, U251 whole cell lysate, MCF-7 whole cell lysate, Rat heart tissue All lanes: ANO2 antibody at 3.9µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 114 kDa Observed band size: 114 kDa IHC image of CSB-PA885689LA01HU diluted at 1:600 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunofluorescence staining of HepG2 cells with CSB-PA885689LA01HU at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) ANO2 Polyclonal antibody

Uniprot No.

Q9NQ90

Target Names

ANO2

Alternative Names

Ano2 antibody; ANO2_HUMAN antibody; Anoctamin 2 antibody; Anoctamin-2 antibody; C12orf3 antibody; Chromosome 12 open reading frame 3 antibody; DKFZp434P102 antibody; Transmembrane protein 16B (eight membrane-spanning domains) antibody; Transmembrane protein 16B antibody

Raised in

Rabbit

Species Reactivity

Human, Rat

Immunogen

Recombinant Human Anoctamin-2 protein (1-83AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The ANO2 Antibody (Product code: CSB-PA885689LA01HU) is Non-conjugated. For ANO2 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA885689LB01HU	ANO2 Antibody, HRP conjugated	ELISA
FITC	CSB-PA885689LC01HU	ANO2 Antibody, FITC conjugated
Biotin	CSB-PA885689LD01HU	ANO2 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:500-1:1000
IF	1:200-1:500

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Customer Reviews and Q&A

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Target Background

Function

Calcium-activated chloride channel (CaCC) which may play a role in olfactory signal transduction. Odorant molecules bind to odor-sensing receptors (OSRs), leading to an increase in calcium entry that activates CaCC current which amplifies the depolarization of the OSR cells, ANO2 seems to be the underlying chloride channel involved in this process. May mediate light perception amplification in retina.

Gene References into Functions

residues facing the putative channel pore are responsible both for controlling the ion selectivity and the gating of the channel, providing an initial understanding of molecular mechanism of ion permeation in TMEM16B PMID: 28046119
data revealed prominently increased autoantibody reactivity against the chloride-channel protein anoctamin 2 (ANO2) in multiple sclerosis cases compared with controls PMID: 26862169
Olfactory function impairment in Italian patients with type 3 von Willebrand disease with partial deletion of TMEM16B. PMID: 25635880
ANO2 is present in human myometrium. PMID: 24928056
The third intracellular loop of TMEM16B is the site involved in calcium ion sensitivity, whereas the C-terminal part affects the rate of transition between the open and the closed state of the channel. PMID: 23570556
VWF and TMEM16B deletions may have a role in severe von Willebrand disease type 3 PMID: 17371490
This study suggested that TMEM16B to be a strong candidate for the long sought-after Ca(2+)-dependent chloride channel in the photoreceptor synapse. PMID: 19474308
Ano2 likely amplifies the odor-induced generator potential in olfactory sensory neuron cilia by sensing elevated calcium levels, permitting the outward flow of chloride ions from the cell. PMID: 19561302
C12orf3, FLJ10261 (ORAOV2), C11orf25 and FLJ34272 constitute a family of eight-transmembrane proteins with N- and C-terminal tails facing the cytoplasm. PMID: 12739008

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Subcellular Location

Cell membrane; Multi-pass membrane protein.

Protein Families

Anoctamin family

Tissue Specificity

Retina, especially in the photoreceptor synaptic terminals.

Database Links

HGNC: 1183

OMIM: 610109

KEGG: hsa:57101

STRING: 9606.ENSP00000314048

UniGene: Hs.148970

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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