ARF1 Antibody

Datasheet
Code CSB-PA957575
Former Code(s) CSB-PA194705
Size US$299
Uniprot No. P84077
Image
  • The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using CSB-PA957575(ARF1 Antibody) at dilution 1/25, on the right is treated with synthetic peptide. (Original magnification: ×200)
  • Gel: 12%SDS-PAGE, Lysate: 40 μg, Lane 1-2: Human fetal brain tissue, Human kidney tissue, Primary antibody: CSB-PA957575(ARF1 Antibody) at dilution 1/500, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 minute
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunogen Synthetic peptide of Human ARF1
Raised in Rabbit
Species Reactivity Human,Mouse,Rat
Tested Applications ELISA,WB,IHC;ELISA:1:1000-1:5000,WB:1:500-1:2000,IHC:1:25-1:100
Relevance ADP-ribosylation factor 1 (ARF1) is a member of the human ARF gene family. The family members encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking as activators of phospholipase D. The gene products, including 6 ARF proteins and 11 ARF-like proteins, constitute a family of the RAS superfamily. The ARF proteins are categorized as class I (ARF1, ARF2 and ARF3), class II (ARF4 and ARF5) and class III (ARF6), and members of each class share a common gene organization. The ARF1 protein is localized to the Golgi apparatus and has a central role in intra-Golgi transport. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene.
Form Liquid
Conjugate Non-conjugated
Storage Buffer -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Purification Method Antigen affinity purification
Isotype IgG
Alias ADP-ribosylation factor 1
Immunogen Species Homo sapiens (Human)
Target Names ARF1
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Still Have Questions? Leave a Message or Start an on-line Chat
Function GTP-binding protein that functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase. Involved in protein trafficking among different compartments. Modulates vesicle budding and uncoating within the Golgi complex. Deactivation induces the redistribution of the entire Golgi complex to the endoplasmic reticulum, suggesting a crucial role in protein trafficking. In its GTP-bound form, its triggers the association with coat proteins with the Golgi membrane. The hydrolysis of ARF1-bound GTP, which is mediated by ARFGAPs proteins, is required for dissociation of coat proteins from Golgi membranes and vesicles. The GTP-bound form interacts with PICK1 to limit PICK1-mediated inhibition of Arp2/3 complex activity; the function is linked to AMPA receptor (AMPAR) trafficking, regulation of synaptic plasicity of excitatory synapses and spine shrinkage during long-term depression (LTD).
Subcellular Location Golgi apparatus, Cytoplasm, perinuclear region, Cell junction, synapse, synaptosome, Cell junction, synapse, postsynaptic cell membrane, postsynaptic density, Membrane, Lipid-anchor
Protein Families Small GTPase superfamily, Arf family
Database Links

HGNC: 652

OMIM: 103180

KEGG: hsa:375

STRING: 9606.ENSP00000272102

UniGene: Hs.286221

Pathway Oxidative Phosphorylation
Phospholipase D signaling pathway
Endocytosis

Related Products

ARF1 Antibodies

ARF1 Antibodies for Homo sapiens (Human)

ARF1 Antibodies for Human

ARF1 Proteins

ARF1 Proteins for Homo sapiens (Human)

Most popular with customers

Newsletters

Get all the latest information on Events, Sales and Offers. Sign up for newsletter today.

Copyright © 2007-2018 www.cusabio.com CUSABIO TECHNOLOGY LLC All Rights Reserved.