ATG101 Antibody

Datasheet

Code	CSB-PA883590LA01HU
Size	US$166
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Image	Immunofluorescence staining of A549 cells with CSB-PA883590LA01HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) ATG101 Polyclonal antibody

Uniprot No.

Q9BSB4

Target Names

ATG101

Alternative Names

ATG101 antibody; Atg13-interacting protein antibody; ATGA1_HUMAN antibody; Autophagy-related protein 101 antibody; C12orf44 antibody; Chromosome 12 open reading frame 44 antibody; FLJ11773 antibody; OTTHUMP00000241687 antibody; OTTHUMP00000241688 antibody; OTTHUMP00000241689 antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Autophagy-related protein 101 protein (1-218AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The ATG101 Antibody (Product code: CSB-PA883590LA01HU) is Non-conjugated. For ATG101 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA883590LB01HU	ATG101 Antibody, HRP conjugated	ELISA
FITC	CSB-PA883590LC01HU	ATG101 Antibody, FITC conjugated
Biotin	CSB-PA883590LD01HU	ATG101 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, IF

Recommended Dilution

Application	Recommended Dilution
IF	1:50-1:200

Protocols

ELISA Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

Autophagy factor required for autophagosome formation. Stabilizes ATG13, protecting it from proteasomal degradation.

Gene References into Functions

Structure of the human Atg13-Atg101 HORMA heterodimer in the ULK1 complex that controls autophagy has been described. PMID: 26299944
Data indicate mitogen lacritin stimulates FOXO3-ATG101 and FOXO1-ATG7 autophagic coupling and restores metabolic homeostasis. PMID: 23640897
The identification of the novel protein, Atg101, and the validation of Atg13 and Atg101 as ULK1-interacting proteins, suggests an Atg1 complex is involved in the induction of macroautophagy in mammalian cells. PMID: 19287211
These data suggest that Atg101 is a novel Atg protein that functions together with ULK, Atg13 and FIP200. PMID: 19597335

Subcellular Location

Cytoplasm. Preautophagosomal structure.

Protein Families

ATG101 family

Database Links

HGNC: 25679

OMIM: 615089

KEGG: hsa:60673

STRING: 9606.ENSP00000338990

UniGene: Hs.9911

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Email: [email protected]
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Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

The Overview of Autophagy Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Related pathways

Autophagy

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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