ATP6V0A1 Antibody

Code CSB-PA846292LA01HU
Size US$299Purchase it in Cusabio online store
(only available for customers from the US)
Image
  • Western Blot
    Positive WB detected in: Rat brain tissue, Mouse brain tissue
    All lanes: ATP6V0A1 antibody at 3μg/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 97, 96 kDa
    Observed band size: 97 kDa

  • Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA846292LA01HU at dilution of 1:100

  • Immunofluorescent analysis of A549 cells using CSB-PA846292LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

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Product Details

Full Product Name Rabbit anti-Homo sapiens (Human) ATP6V0A1 Polyclonal antibody
Uniprot No. Q93050
Target Names ATP6V0A1
Alternative Names a1 antibody; ATP6N1 antibody; ATP6N1A antibody; ATP6V0A1 antibody; ATPase H+ transporting lysosomal (vacuolar proton pump) non catalytic accessory protein 1A (110/116kD) antibody; ATPase H+ transporting lysosomal non catalytic accessory protein 1 (110/116kD) antibody; ATPase H+ transporting lysosomal V0 subunit a1 antibody; ATPase H+ transporting V0 subunit a1 antibody; ATPase, H+ transporting, lysosomal, noncatalytic accessory protein 1A antibody; Clathrin coated vesicle/synaptic vesicle proton pump 116 kDa subunit antibody; Clathrin-coated vesicle/synaptic vesicle proton pump 116 kDa subunit antibody; DKFZp781J1951 antibody; H(+) transporting two sector ATPase 116 kDa accessory protein A1 antibody; Stv1 antibody; V ATPase 116 kDa antibody; V ATPase 116 kDa isoform a1 antibody; V type proton ATPase 116 kDa subunit a antibody; V type proton ATPase 116 kDa subunit a isoform 1 antibody; V-ATPase 116 kDa isoform a1 antibody; V-type proton ATPase 116 kDa subunit a isoform 1 antibody; Vacuolar adenosine triphosphatase subunit Ac116 antibody; Vacuolar proton pump subunit 1 antibody; Vacuolar proton pump, subunit 1 antibody; Vacuolar proton translocating ATPase 116 kDa subunit A antibody; Vacuolar proton translocating ATPase 116 kDa subunit a isoform 1 antibody; Vacuolar type H(+) ATPase 115 kDa subunit antibody; Vph1 antibody; VPP1 antibody; VPP1_HUMAN antibody
Raised in Rabbit
Species Reactivity Human, Mouse, Rat
Immunogen Recombinant Human V-type proton ATPase 116 kDa subunit a isoform 1 protein (80-260AA)
Immunogen Species Homo sapiens (Human)
Conjugate Non-conjugated

The ATP6V0A1 Antibody (Product code: CSB-PA846292LA01HU) is Non-conjugated. For ATP6V0A1 Antibody with conjugates, please check the following table.

Available Conjugates
Conjugate Product Code Product Name Application
HRP CSB-PA846292LB01HU ATP6V0A1 Antibody, HRP conjugated ELISA
FITC CSB-PA846292LC01HU ATP6V0A1 Antibody, FITC conjugated
Biotin CSB-PA846292LD01HU ATP6V0A1 Antibody, Biotin conjugated ELISA
Clonality Polyclonal
Isotype IgG
Purification Method >95%, Protein G purified
Concentration It differs from different batches. Please contact us to confirm it.
Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form Liquid
Tested Applications ELISA, WB, IHC, IF
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:5000
IHC 1:20-1:200
IF 1:50-1:200
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Background

Function
Required for assembly and activity of the vacuolar ATPase. Potential role in differential targeting and regulation of the enzyme for a specific organelle.
Gene References into Functions
  1. The function of vacuolar ATPase (V-ATPase) a subunit isoforms in invasiveness of MCF10a and MCF10CA1a human breast cancer cells. PMID: 24072707
  2. Inhibition of lysosome degradation on autophagosome formation and responses to GMI, an immunomodulatory protein from Ganoderma microsporum. ATP6V0A1 plays an important role in mediating autophagosome-lysosome fusion. PMID: 22708544
  3. a series of events whereby ATP6V0A1 3'-UTR variant T+3246C functioned: ATP6V0A1 expression probably was affected through differential micro-RNA effects, altering vacuolar pH and consequently CHGA processing and exocytotic secretion. PMID: 21558123
  4. The mRNA levels of POLR2F, ATP6V0A1 and PRNP were evaluated by quantitative RT-PCR in 70 colorectal carcinomas and 17 normal tissue specimens and were correlated with clinicopathological parameters. PMID: 18505059
Subcellular Location Cytoplasmic vesicle membrane; Multi-pass membrane protein. Melanosome. Note=Coated vesicle. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
Protein Families V-ATPase 116 kDa subunit family
Database Links

HGNC: 865

OMIM: 192130

KEGG: hsa:535

STRING: 9606.ENSP00000264649

UniGene: Hs.463074

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