ATP6V1E2 Antibody

Code CSB-PA836175LA01HU
Size US$166
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Image
  • Immunofluorescence staining of HepG2 cells with CSB-PA836175LA01HU at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Product Details

Full Product Name
Rabbit anti-Homo sapiens (Human) ATP6V1E2 Polyclonal antibody
Uniprot No.
Target Names
Alternative Names
ATP6V1E2 antibody; ATPase, H+ transporting, lysosomal 31kDa, V1 subunit E2 antibody; V-ATPase subunit E 2 antibody; V-type proton ATPase subunit e 2 antibody; Vacuolar proton pump subunit e 2 antibody; vacuolar type proton-translocating ATPase subunit E1 antibody; VATE2_HUMAN antibody
Raised in
Rabbit
Species Reactivity
Human
Immunogen
Recombinant Human V-type proton ATPase subunit E 2 protein (1-226AA)
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated

The ATP6V1E2 Antibody (Product code: CSB-PA836175LA01HU) is Non-conjugated. For ATP6V1E2 Antibody with conjugates, please check the following table.

Available Conjugates
Conjugate Product Code Product Name Application
HRP CSB-PA836175LB01HU ATP6V1E2 Antibody, HRP conjugated ELISA
FITC CSB-PA836175LC01HU ATP6V1E2 Antibody, FITC conjugated
Biotin CSB-PA836175LD01HU ATP6V1E2 Antibody, Biotin conjugated ELISA
Clonality
Polyclonal
Isotype
IgG
Purification Method
>95%, Protein G purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Form
Liquid
Tested Applications
ELISA, IF
Recommended Dilution
Application Recommended Dilution
IF 1:50-1:200
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Usage
For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function
Subunit of the peripheral V1 complex of vacuolar ATPase essential for assembly or catalytic function. V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells. This isoform is essential for energy coupling involved in acidification of acrosome.
Gene References into Functions
  1. Rat vacuolar H(+)ATPase associates with NHE-RF (Na(+)/H(+) exchanger regulatory factor); the E subunit was co-immunoprecipitated from rat kidney cytosol with NHE-RF antibodies. PMID: 10748165
Protein Families
V-ATPase E subunit family
Tissue Specificity
Testis specific.
Database Links

HGNC: 18125

OMIM: 617385

KEGG: hsa:90423

STRING: 9606.ENSP00000304891

UniGene: Hs.437691

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