BDH1 Antibody

Datasheet

Citations (1)

Code	CSB-PA002648LA01HU
Size	$600
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Image	Western Blot Positive WB detected in:Mouse Heart tissue lysate, Mouse Liver tissue lysate, Mouse Brain tissue lysate, Rat Heart tissue lysate, Rat Livert tissue lysate, Rat Brain tissue lysate, MCF7 whole cell lysate All lanes: BDH1 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 39 kDa Observed band size: 31 kDa IHC image of CSB-PA002648LA01HU diluted at 1:150 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody IHC image of CSB-PA002648LA01HU diluted at 1:150 and staining in paraffin-embedded mouse liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody Immunofluorescence staining of MCF-7 cell with CSB-PA002648LA01HU at 1:20, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Immunofluorescence staining of MCF-7 cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Immunofluorescence staining of U251 cell with CSB-PA002648LA01HU at 1:20, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Immunofluorescence staining of U251 cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Product Details
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) BDH1 Polyclonal antibody

Uniprot No.

Q02338

Target Names

BDH1

Alternative Names

3 hydroxybutyrate dehydrogenase (heart; mitochondrial) antibody; 3-hydroxybutyrate dehydrogenase antibody; BDH antibody; BDH_HUMAN antibody; BDH1 antibody; D beta hydroxybutyrate dehydrogenase; mitochondrial antibody; D-beta-hydroxybutyrate dehydrogenase antibody; mitochondrial antibody

Raised in

Rabbit

Species Reactivity

Human, Mouse,Rat

Immunogen

Recombinant Human D-beta-hydroxybutyrate dehydrogenase, mitochondrial protein (47-343AA)

Immunogen Species

Homo sapiens (Human)

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen affinity purification

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.02% sodium azide
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:1000-1:3000
IHC	1:100-1:300
IF	1:20-1:100

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

The BDH1 polyclonal antibody is a type of antibody generated in rabbits using recombinant human D-beta-hydroxybutyrate dehydrogenase, mitochondrial protein (47-343aa) as an immunogen. This antibody has been purified using Protein G, resulting in a purity level greater than 95%. This ensures that the antibody is of high quality and free from impurities that may interfere with experimental results.

The BDH1 polyclonal antibody can recognize BDH1 protein in both human and mouse samples. The BDH1 polyclonal antibody has been tested in a range of applications, including ELISA, WB, and IHC, for the quantitative or qualitative detection, determination of the presence and size, detection of the localization and expression levels of BDH1 protein.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Citations

Lysine β-hydroxybutyrylation promotes lipid accumulation in alcoholic liver disease N Chen,Biochemical pharmacology,2023

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Target Background

Gene References into Functions

Data show that the ketone body metabolizing enzymes BDH1, BDH2, OXCT1 and ACAT1 were expressed at the mRNA and protein level in all glioma cell lines. PMID: 21791085

Subcellular Location

Mitochondrion inner membrane. Mitochondrion matrix.

Protein Families

Short-chain dehydrogenases/reductases (SDR) family

Database Links

HGNC: 1027

OMIM: 603063

KEGG: hsa:622

STRING: 9606.ENSP00000350914

UniGene: Hs.274539

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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