Product Details

Purity

Greater than 90% as determined by SDS-PAGE.

Target Names

BDH1

Uniprot No.

Q02338

Research Area

Cancer

Alternative Names

3 hydroxybutyrate dehydrogenase (heart; mitochondrial); 3-hydroxybutyrate dehydrogenase; BDH; BDH_HUMAN; BDH1; D beta hydroxybutyrate dehydrogenase; mitochondrial; D-beta-hydroxybutyrate dehydrogenase; mitochondrial

Species

Homo sapiens (Human)

Source

E.coli

Expression Region

47-343aa

Target Protein Sequence

ASAAEPVGSKAVLVTGCDSGFGFSLAKHLHSKGFLVFAGCLMKDKGHDGVKELDSLNSDRLRTVQLNVCSSEEVEKVVEIVRSSLKDPEKGMWGLVNNAGISTFGEVEFTSLETYKQVAEVNLWGTVRMTKSFLPLIRRAKGRVVNISSMLGRMANPARSPYCITKFGVEAFSDCLRYEMYPLGVKVSVVEPGNFIAATSLYSPESIQAIAKKMWEELPEVVRKDYGKKYFDEKIAKMETYCSSGSTDTSPVIDAVTHALTATTPYTRYHPMDYYWWLRMQIMTHLPGAISDMIYIR
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

37.1kDa

Protein Length

Full Length of Mature Protein

Tag Info

N-terminal 6xHis-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

The region for expressing recombinant Human BDH1 contains amino acids 47-343. This BDH1 protein is theoretically predicted to have a molecular weight of 37.1 kDa. This BDH1 protein is produced using e.coli expression system. The N-terminal 6xHis tag was fused into the coding gene segment of BDH1, making it easier to detect and purify the BDH1 recombinant protein in the later stages of expression and purification.

The research on BAG family molecular chaperone regulator 2 (BAG2) covers multiple fields, including energy metabolism, obesity, diabetes, and neuroscience. It primarily engages in intracellular energy metabolism, particularly associated with ketone body synthesis and metabolism. In studies of metabolic disorders such as obesity and diabetes, scientists have identified the crucial role of BAG2 in regulating the levels of D-β-hydroxybutyrate in the body. This is closely linked to the significance of ketone bodies as an alternative energy source during periods of starvation and physical activity. Additionally, BAG2 is implicated in the functioning of the nervous system, with some research suggesting its potential involvement in the pathogenesis of neurodegenerative diseases.

Target Background

Gene References into Functions

Data show that the ketone body metabolizing enzymes BDH1, BDH2, OXCT1 and ACAT1 were expressed at the mRNA and protein level in all glioma cell lines. PMID: 21791085

Subcellular Location

Mitochondrion inner membrane. Mitochondrion matrix.

Protein Families

Short-chain dehydrogenases/reductases (SDR) family

Database Links

HGNC: 1027

OMIM: 603063

KEGG: hsa:622

STRING: 9606.ENSP00000350914

UniGene: Hs.274539

Code	CSB-EP002648HU
Abbreviation	Recombinant Human BDH1 protein
MSDS	MSDS Datasheet
Size	$224
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Recombinant Human D-beta-hydroxybutyrate dehydrogenase, mitochondrial (BDH1)

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