BLVRB Antibody

Datasheet

Code	CSB-PA002722HA01HU
Size	US$166
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Image	Western Blot Positive WB detected in: K562 whole cell lysate, PC3 whole cell lysate, HepG2 whole cell lysate All lanes: BLVRB antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 23 kDa Observed band size: 23 kDa IHC image of CSB-PA002722HA01HU diluted at 1:300 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunofluorescence staining of Hela cells with CSB-PA002722HA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) BLVRB Polyclonal antibody

Uniprot No.

P30043

Target Names

BLVRB

Alternative Names

Biliverdin IX beta reductase antibody; biliverdin reductase B (flavin reductase (NADPH)) antibody; Biliverdin reductase B antibody; Biliverdin-IX beta-reductase antibody; BLVRB antibody; BLVRB_HUMAN antibody; BVR B antibody; BVR-B antibody; BVRB antibody; epididymis secretory protein Li 10 antibody; Flavin reductase (NADPH) antibody; Flavin reductase antibody; FLR antibody; FR antibody; GHBP antibody; Green heme binding protein antibody; Green heme-binding protein antibody; HEL-S-10 antibody; Methemoglobin reductase antibody; MGC117413 antibody; NADPH dependent diaphorase antibody; NADPH flavin reductase antibody; NADPH-dependent diaphorase antibody; NADPH-flavin reductase antibody; SDR43U1 antibody; short chain dehydrogenase/reductase family 43U; member 1 antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Flavin reductase (NADPH) protein (2-206AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The BLVRB Antibody (Product code: CSB-PA002722HA01HU) is Non-conjugated. For BLVRB Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA002722HB01HU	BLVRB Antibody, HRP conjugated	ELISA
FITC	CSB-PA002722HC01HU	BLVRB Antibody, FITC conjugated
Biotin	CSB-PA002722HD01HU	BLVRB Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:1000-1:5000
IHC	1:200-1:500
IF	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Background

Function

Broad specificity oxidoreductase that catalyzes the NADPH-dependent reduction of a variety of flavins, such as riboflavin, FAD or FMN, biliverdins, methemoglobin and PQQ (pyrroloquinoline quinone). Contributes to heme catabolism and metabolizes linear tetrapyrroles. Can also reduce the complexed Fe(3+) iron to Fe(2+) in the presence of FMN and NADPH. In the liver, converts biliverdin to bilirubin.

Gene References into Functions

These results provide promising chemical scaffolds for the development of enhanced BLVRB inhibitors and identify chemical probes to better dissect the role of biliverdins, alternative substrates, and BLVRB function in physiologically relevant cellular contexts. PMID: 29487133
These data define the first physiologically relevant function of BLVRB and implicate its activity and/or heme-regulated BV tetrapyrrole(s) in a unique redox-regulated bioenergetic pathway. PMID: 27207795
miR-127-5p suppressed NF-kappaB activity by directly targeting BLVRB in hepatocellular carcinoma cells. PMID: 26708147
quantum mechanics/molecular mechanics potential energy surfaces show that the lowest energy pathway proceeds with a positively charged pyrrole intermediate via two transition states PMID: 18241201

Subcellular Location

Cytoplasm.

Tissue Specificity

Predominantly expressed in liver and erythrocytes. At lower levels in heart, lung, adrenal gland and cerebrum.

Database Links

HGNC: 1063

OMIM: 600941

KEGG: hsa:645

STRING: 9606.ENSP00000263368

UniGene: Hs.515785

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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