BRCA2 Antibody

Code CSB-PA002796GA01HU
Size US$685
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Product Details

Uniprot No. P51587
Target Names BRCA2
Alternative Names BRCA 2 antibody; BRCA1/BRCA2 containing complex subunit 2 antibody; Brca2 antibody; BRCA2; DNA repair associated antibody; BRCA2_HUMAN antibody; BRCC 2 antibody; BRCC2 antibody; Breast and ovarian cancer susceptibility gene early onset antibody; breast and ovarian cancer susceptibility protein 2 antibody; Breast cancer 2 early onset antibody; Breast Cancer 2 tumor suppressor antibody; Breast cancer susceptibility protein BRCA2 antibody; Breast cancer type 2 susceptibility protein antibody; BROVCA2 antibody; FACD antibody; FAD 1 antibody; FAD antibody; FAD1 antibody; FANCB antibody; FANCD 1 antibody; FANCD antibody; FANCD1 antibody; FANCD1 gene antibody; Fanconi anemia complementation group D1 antibody; Fanconi anemia group D1 protein antibody; GLM3 antibody; mutant BRCA2 antibody; OTTHUMP00000018803 antibody; OTTHUMP00000042401 antibody; PNCA2 antibody; XRCC11 antibody
Raised in Rabbit
Species Reactivity Human
Immunogen Human BRCA2
Immunogen Species Homo sapiens (Human)
Isotype IgG
Purification Method Antigen Affinity purified
Concentration It differs from different batches. Please contact us to confirm it.
Buffer PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. -20°C, Avoid freeze / thaw cycles.
Tested Applications ELISA,WB
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Citations

Quinacrine based gold hybrid nanoparticle caused apoptosis through modulating replication fork in oral cancer stem cells. KC Hembram,Mol. Pharm,2020

Applications: Western Blot
Review: We checked the expression of BRCA-2 post NP treatment for different time periods (48, 72 and 96 h). It was noted that no significant change in BRCA-2 expression was found whereas RAD-51 level increased significantly (up to 3 fold in 96 h).
PMID: 32407635

Target Data

Function Involved in double-strand break repair and/or homologous recombination. Binds RAD51 and potentiates recombinational DNA repair by promoting assembly of RAD51 onto single-stranded DNA (ssDNA). Acts by targeting RAD51 to ssDNA over double-stranded DNA, enabling RAD51 to displace replication protein-A (RPA) from ssDNA and stabilizing RAD51-ssDNA filaments by blocking ATP hydrolysis. Part of a PALB2-scaffolded HR complex containing RAD51C and which is thought to play a role in DNA repair by HR. May participate in S phase checkpoint activation. Binds selectively to ssDNA, and to ssDNA in tailed duplexes and replication fork structures. May play a role in the extension step after strand invasion at replication-dependent DNA double-strand breaks; together with PALB2 is involved in both POLH localization at collapsed replication forks and DNA polymerization activity. In concert with NPM1, regulates centrosome duplication. Interacts with the TREX-2 complex (transcription and export complex 2) subunits PCID2 and SEM1, and is required to prevent R-loop-associated DNA damage and thus transcription-associated genomic instability. Silencing of BRCA2 promotes R-loop accumulation at actively transcribed genes in replicating and non-replicating cells, suggesting that BRCA2 mediates the control of R-loop associated genomic instability, independently of its known role in homologous recombination
Gene References into Functions
  1. Evidence has been provided that the majority of the Cas9-induced single nicks at the target DNA strand rely on RAD51 and BRCA2 for efficient and scar-less DNA repair. PMID: 28067217
  2. It has been shown that BRCA2-mutant prostate cancer cells harbor increased genomic instability and a mutational profile that more closely resembles metastatic than localized disease. PMID: 28067867
  3. Germline mutations involving the Fanconi anemia pathway, such as BRCA2 are often implicated in Invasive Pancreatic Ductal Adenocarcinoma. PMID: 28870368
  4. The authors propose that BRCA2 antagonizes 53BP1, RIF1, and Artemis-dependent c-nonhomologous end-joining and alt-nonhomologous end-joining to prevent gross genomic instability in a RAD51-independent manner. PMID: 29133916
  5. region in the N terminus exhibits DNA binding activity and promotes RAD51-mediated homologous recombination PMID: 27628236
  6. BRCA2 germ line mutation is associated with unilateral triple-negative breast cancer. PMID: 29514593
  7. BRCA2 germ line mutation is associated with ovarian cancer. PMID: 29506471
  8. BRCA1/2 mutations are not uncommon among selected Jordanian females with breast cancer. PMID: 29409476
  9. male BRCA1/2 mutation carriers with breast and prostate cancer demonstrated a favorable 5-year survival. PMID: 29433453
  10. BRCA2 SNP is associated with Enhanced response rate to pegylated liposomal doxorubicin in high grade serous ovarian carcinomas. PMID: 29298688
  11. this study identifies the importance of TDP1 as a novel determinant of response to CNDAC across various cancer types (especially non-small cell lung cancers), and demonstrates the differential involvement of BRCA2, PARP1, and TDP1 in the cellular responses to CNDAC, AraC, and CPT PMID: 28802254
  12. No evidence for a an association between the genotype at BRCA2 c.6937 + 594T>G and breast cancer risk. PMID: 29356578
  13. the physiological significance of GIPC3 as a genetic interactor of BRCA2 is supported by the observation that Brca2-null embryos with Gipc3 overexpression are developmentally more advanced than their control littermates. Taken together, we have uncovered a novel role for GIPC3 as a BRCA2 genetic interactor. PMID: 29021281
  14. BRCA2 mutation carriers showed earlier onset age of breast tumor and higher risk of developing contra lateral breast cancer in women from Murcia (south-eastern Spain). PMID: 28477318
  15. Results, including a comprehensive co-segregation analysis, indicate that the novel duplication identified has a pathogenic role and would be considered a causing-disease variant in genetic and oncologic counseling. PMID: 28620890
  16. In our EOBC cohort, a contralateral breast cancer was diagnosed in ~60% of BRCA1/2 carriers, but only in 8% of non-carriers. These findings show that BRCA1/2 mutations overwhelmingly lead to a contralateral disease when breast cancer is diagnosed at early age. PMID: 27726213
  17. Results showed that BRCA2 is down-regulated in epithelial ovarian cancer by lncRNA RP11-552M11.4 which promotes cell proliferation, migration and invasion. PMID: 29478268
  18. BRCA2 and CHEK2 play an important role in the genetic susceptibility to urinary tract cancers. PMID: 27632928
  19. The present study shows that the G allele carriers of BRCA2 rs9534275 were associated with increased serum total cholesterol and ApoB levels in the coronary artery disease patients and increased risk of coronary artery disease and ischemic stroke. PMID: 28982360
  20. BRCA2 rs144848 polymorphism is associated with cancer. PMID: 28418854
  21. Tumours arising in BRCA2 mutation carriers showed significantly higher methylation of candidate genes, than those arising in non-BRCA2 familial MBCs (average AMI 23.6 vs 16.6, p = 0.01, 45% of genes hypermethylated vs 34%, p < 0.01). PMID: 28893223
  22. role of BRCA2 splicing for acquired chemo-resistance in BRCA2 mutation associated malignancy PMID: 28617445
  23. Results show frequent BRCA2, EGFR, and NTRK1/2/3 mutations in mismatch repair-deficient colorectal cancers , sugggesting personalized medicine strategies to treat the patients with advanced disease who may have no remaining treatment options. PMID: 28591715
  24. In this study, we evaluated this novel mechanism of drug resistance in newly diagnosed, early-stage BRCA1/2-mutant breast cancer patients who had a poor response to platinum-based neoadjuvant chemotherapy. PMID: 28087643
  25. IGH/MYC-positive Burkitt lymphoma/leukemia cells have decreased BRCA2 and are sensitive to PARP1 inhibition alone or in combination with other chemotherapies. This study postulates that IGH/MYC-induced BRCA2 deficiency may predispose Burkitt lymphoma cells to synthetic lethality triggered by PARP1 inhibitors. PMID: 28634224
  26. This meta-analysis showed that a BRCA2 mutation predicted poor survival outcomes in patients with prostate cancer, especially in those undergoing treatments with radiotherapy. Therefore, the use of BRCA2 mutation as a clinical prognostic factor could help stratify the high-risk patients and provide clinical strategies for more effective targeted treatments for patients with prostate cancer. PMID: 28410213
  27. The clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 system was employed to target and correct a FANCD1 gene deletion. The study shows the ability to correct a patient mutation in primary FANCD1 cells in a precise manner. PMID: 28613254
  28. Data indicate that c.1310_1313delAAGA mutation of BRCA2 gene is recurrent with high frequency in patients from the North-East region of Morocco. PMID: 28577564
  29. Patients with BRCA2 mutation were almost 25 times more likely to have chronic pancreatitis-like changes compared with sex-matched controls. PMID: 28375947
  30. Prevalence of pathogenic and likely pathogenic variants in the hotspots regions of BRCA2 was 23 and 6.3 % respectively in this cohort PMID: 28039656
  31. We present two cases of black South African patients with FA diagnosed with biallelic BRCA2 mutations and discuss the phenotypic consequences and implications for them and their families PMID: 28185119
  32. High BRCA2 expression is associated with drug resistance in ovarian cancer. PMID: 26959114
  33. IMPACT data were consistent with increased risks of onset among BRCA1 and BRCA2 mutation carriers PMID: 27742670
  34. In the very high-risk Bladder Cancer patients , several genes had a higher frequency of mutations than reported in the The Cancer Genome Atlas database, including BRCA2 . Mutation associations with receipt of neoadjuvant chemotherapy, nodal involvement, metastatic disease development, and survival were analyzed. PMID: 27520487
  35. three patients had biallelic inactivation of BRCA2, a tumor suppressor gene critical for homologous DNA repair. two had germline BRCA2 mutations. The third patient had somatic BRCA2 homozygous copy loss. Biallelic BRCA2 inactivation in Metastatic Castration-resistant Prostate Cancer warrants further exploration as a predictive biomarker for sensitivity to platinum c PMID: 26724258
  36. The current model places BRCA2 as a central regulator of genome stability by repairing DNA double strand breaks and limiting replication stress. [review] PMID: 27530658
  37. Increasing evidence on the molecular role of the BRCA2 protein in the homologous recombination of DNA damages suggest that BRCA2-related PDAC are sensitive to agents causing DNA cross-linking damage, such as platinum salts, and treatments targeting rescue DNA repair pathways, such as poly(ADP-ribose) polymerase inhibitors that are currently under investigation.[ review] PMID: 27511924
  38. Data show that BRCA2 was required for HDAC2/3 association with acetylated BubR1 in nocodazole (Noc)-arrested cells. PMID: 28985013
  39. Data show that mammary epithelial cells are inviable upon BRCA2 loss, which leads to replication stress associated with under replication, causing mitotic abnormalities and G1 arrest. PMID: 28904335
  40. We demonstrated an association between six previously published single nucleotide polymorphisms (rs15869 [ BRCA2], rs1805389 [ LIG4], rs8079544 [ TP53], rs25489 [ XRCC1], rs1673041 [ POLD1], and rs11615 [ ERCC1]) and subsequent CNS tumors in survivors of childhood cancer treated by radiation therapy. PMID: 28976792
  41. Patients with Fanconi anemia have higher incidences of ectopic neurohypophysis, adenohypophysis hypoplasia, platybasia and other midline central nervous system skull base posterior fossa abnormalities than age- and sex-matched controls. Patients with posterior fossa abnormalities, including pontocerebellar hypoplasia, are more likely to have biallelic BRCA2 mutations. PMID: 28283722
  42. CDNA representing BRCA2 alternate splice sites was amplified. PMID: 27060066
  43. WTIP interacts with BRCA2 and might be responsible for BRCA2 centrosome localization in cervical cancer cell. PMID: 27535760
  44. Founder mutations in BRCA2 contribute to an increased risk of ovarian and breast cancer in the western Danish population. PMID: 26833046
  45. BRCA2 germline mutation is associated with triple-negative breast cancer. PMID: 27553291
  46. BRCA2 c.68-7T>A pathogenic mutation from Norwegian breast or ovarian cancer cohort PMID: 27495310
  47. Architectural plasticity of human BRCA2-RPA-RAD51 complexes in DNA break repair has been described. PMID: 28168276
  48. These findings suggest that R2787H variant of BRCA2 could have potential functional impact. PMID: 27211102
  49. Even if a BRCA2 mutation is already identified within a family, the presence of early onset breast cancer affected non-carriers hampers accurate risk estimates for both mutation carriers and other negative family members. PMID: 28199346
  50. This study detected monoallelic L1053X mutation causing the same stop codon in BRCA2 protein sequence at the same position in four Sudanese female breast cancer patients out of nine from different families. PMID: 28814288
  51. A patient with an ampulla of Vater carcinoma was incidentally found to carry the BRCA2 c.156_157insAlu mutation. Further testing of a consecutive series of additional 15 ampullary carcinomas for BRCA1/BRCA2 mutations using a combination of direct founder mutation testing and full gene analysis with next generation sequencing. BRCA2 mutations were observed with a frequency of 14.3% in ampulla of Vater carcinomas. PMID: 27532258
  52. radiosensitization was evaluated using the glioblastoma cell line, U87MG-E6, which harbors inactivated p53, in comparison with the cell line, HCT116 p53 (-/-). We conclude that radiosensitization by arsenite is related to ROS and BRCA2 function. PMID: 28739695
  53. we generated a Brca2 knock-in mouse model lacking exons 4-7 and demonstrated that these exons are dispensable for viability as well as tumor-free survival. This study provides the first in vivo evidence of the functional significance of a minor transcript of BRCA2 that can play a major role in the survival of humans who are homozygous for a clearly pathogenic mutation. PMID: 26920070
  54. Data suggest that modulation of histone deacetylase (HAT) activity by an SNP in BRCA2 (breast cancer type 2 susceptibility protein; rs144848, 1342A>C, N372H) is a plausible mechanism of paclitaxel resistance in breast cancer; after HAT inhibitor treatment, HAT activity, and paclitaxel sensitivity is restored in heterozygous BRCA2 variant breast cancer cells. PMID: 28431939
  55. One rare variant in BRCA2 3'UTR was identified in 716 breast cancer cases and 619 controls. Identified variant gives no convincing evidence of potential pathogenicity. PMID: 26785832
  56. Germline mutation in BRCA2 gene is associated with melanoma. PMID: 27074266
  57. In this review, BRCA2 mutation carriers are more likely to have poor risk of prostate cancer and exhibit worse outcomes. PMID: 27225637
  58. BRCA2 mutation carriers one region, up to 45 kb upstream of BNC2, and containing 100 correlated SNPs was identified as candidate causal PMID: 27463617
  59. Study reports that exposure to naturally occurring concentrations of formaldehyde or acetaldehyde selectively unmasks genomic instability in cells heterozygous for multiple, clinically relevant, truncating BRCA2 mutations. PMID: 28575672
  60. ubiquitination of RAD51 hinders RAD51-BRCA2 interaction, while deubiquitination of RAD51 facilitates RAD51-BRCA2 binding and RAD51 recruitment and thus is critical for proper homologous recombination PMID: 27941124
  61. These results suggest that cytoplasmic dynein 1 binds to BRCA2 through the latter's centrosomal localization signal and BRCA2 mediates the cohesion between centrosomes during the S phase, potentially serving as a cell-cycle checkpoint. PMID: 27433848
  62. Results indicate that aberrant splicing is especially prevalent in breast cancer 2 protein (BRCA2) exons 17 and 18. PMID: 28339459
  63. BRCA2 mutation carriers are at increased risk developing insulin resistance and type-II diabetes mellitus; have an altered expression of circulating proteins associated with venous thrombo-embolism (Review) PMID: 27451331
  64. BRCA2 reversion mutation is associated with resistance to PARP inhibitor-based therapy in a pancreatic ductal adenocarcinoma. PMID: 28291774
  65. Study suggests an increased risk of leukaemia in women with a BRCA2 mutation who receive chemotherapy for breast cancer. PMID: 26986251
  66. Structure-activity relationship of the peptide binding-motif mediating the BRCA2:RAD51 protein-protein interaction, is reported. PMID: 26992456
  67. we describe a genetic approach to examine the functional significance of the interaction between BRCA2 and PALB2 by generating a knock-in mouse model of Brca2 carrying a single amino acid change (Gly25Arg, Brca2G25R) that disrupts this interaction. In addition, we have combined Brca2G25R homozygosity as well as hemizygosity with Palb2 and Trp53 heterozygosity . PMID: 27490902
  68. The Single-molecule molecular inversion probes (smMIP)-based genetic testing enables automated and reliable analysis of the coding sequences of BRCA2. PMID: 27974384
  69. the purified RAD51 G151D protein directly and significantly enhances DNA strand exchange activity in the presence of RPA. In concordance with this result, co-incubation of G151D with BRCA2 resulted in a much higher level of strand-exchange activity compared to WT RAD51. Strikingly, the RAD51 G151D variant confers resistance to multiple DNA damaging agents, including ionizing radiation, mitomycin C, and doxorubicin. PMID: 27513445
  70. BRCA2 Mutations are associated with Aggressive Prostate Tumors. PMID: 27087322
  71. 22 different BRCA2 uncertain significance were detected and p.M784V in BRCA2 was the most common uncertain significance. PMID: 28351343
  72. We found an approximately approximately 3 fold increased rate of high RS among BRCA1 and 2 carriers with ER positive breast cancer (BC)compared with the general BC population. PMID: 28120435
  73. BRCA2-truncated protein resulted from the frame shift mutation was found to lose two putative nuclear localization signals and a Rad51-binding motif in the extreme C-terminal region by bioinformatic prediction PMID: 28058502
  74. soluble alpha-fetoprotein is a marker for Fanconi anemia; FANC proteins form multi-protein complexes with BRCA proteins and are involved in homologous DNA repair [review] PMID: 27690720
  75. These results suggested that LIMD1 is a novel BRCA2-interacting protein and is involved in the centrosome localization of BRCA2 and suppression of LIMD1, causing abnormal cell division in EC cells. PMID: 27656835
  76. Findings suggest that the miRNA-binding SNPs in BRCA1/BRCA2 and their interaction with reproductive factors might contribute to BC risk, and miR-627 might down-regulate BRCA2 expression in MCF-7 and MDA-MB-231 cells. PMID: 27807724
  77. Reduction in BRCC2 expression induced by miR-603 was responsible for the enhanced colony formation and proliferative ability noted in the MG-63 and U2OS cells. PMID: 27035098
  78. radiosensitization was at least partly due to the induction of apoptosis, which was only observed in the RKO cells and which may have been induced by BRCA2 degradation or different types of chromosomal aberrations. PMID: 27246457
  79. Association of BRCA2 single nucleotide polymorphisms with the breast cancer risk in the Bangladeshi population PMID: 27068824
  80. that BRCA1/2 multicenter mutation testing is well feasible in formalin-fixed and paraffin-embedded tumor tissue PMID: 27003155
  81. This is the first reported case of adrenocortical carcinoma associated with a BRCA2 germline mutation. PMID: 27603373
  82. the present systematic review collects and analyses the role of BRCA2 alterations to be used in early diagnosis of an inherited syndrome associated with familiar cancer and as a prognostic and predictive biomarker for the management of pancreatic cancer patients. PMID: 28078281
  83. Germline mutations in BRCA2 are not associated with reduced ovarian reserve as measured by Anti-Mullerian hormone. PMID: 27094481
  84. BRCA2 mutations strongly increase the in vitro- and in vivo-sensitivity of cancer cells towards TRAIL-R-mediated apoptosis. PMID: 26843614
  85. Germline mutation in BRCA2 gene is associated with familial breast cancer. PMID: 26824983
  86. BRCA2 mutation is associated with Familial Pancreatic Cancer. PMID: 26727920
  87. BRCA2 mutations are associated with increased plasma levels of thrombo-coagulating-related proteins, which are independent to breast cancer development PMID: 26446551
  88. BRCA2 mutation is associated with perturbation in telomere biology resulting in breast cancer. PMID: 26515461
  89. BRCA2 mutation is associated with risk of breast and ovarian cancer. PMID: 26299336
  90. BRCA2 mutation is associated with pancreatico-biliary neoplasms. PMID: 26004055
  91. the current pathogenic mutation spectrum of the BRCA2 gene in patients with breast cancer in various Asian populations (review). PMID: 26187060
  92. The rs144848 mutation of BRCA2 may have a protective effect against lung cancer among women PMID: 26979245
  93. After miR-17-5p transfection, cell proliferation, migration, invasion and tumorigenesis in the osteosarcoma cells were significantly promoted. We further demonstrated that BRCC2 is a direct target of miR-17-5p. PMID: 26750490
  94. BRCA2 mutation is associated with Pancreatic Cancer. PMID: 26483394
  95. 39 were BRCA1/2 mutation carriers. PMID: 26749359
  96. The RNA effect of 28 BRCA1 and BRCA2 VUS identified in our set of HBOCS families. PMID: 26780556
  97. Lung adenocarcinomas in both male and female patients were associated with (a) genotypic polymorphisms of FANCC and FANCD1. PMID: 26842001
  98. Hypermethylation and low Expression of BRCA2 is associated with Epithelial Ovarian Cancers PMID: 26745123
  99. Breast cancer risk for Korean women with germline mutations in BRCA2. PMID: 26195121
  100. There was no significant difference in disease-free survival between BRCA2 mutation carriers and patients with sporadic disease. (Review, meta-analysis) PMID: 26489613

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Involvement in disease Breast cancer (BC); Pancreatic cancer 2 (PNCA2); Breast-ovarian cancer, familial, 2 (BROVCA2); Fanconi anemia complementation group D1 (FANCD1); Glioma 3 (GLM3)
Subcellular Location Nucleus, Cytoplasm, cytoskeleton, microtubule organizing center, centrosome
Tissue Specificity Highest levels of expression in breast and thymus, with slightly lower levels in lung, ovary and spleen.
Database Links

HGNC: 1101

OMIM: 114480

KEGG: hsa:675

STRING: 9606.ENSP00000369497

UniGene: Hs.34012

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