BRD9 Antibody

Code CSB-PA888000ESR2HU
Size US$166
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Image
  • Western blot
    All lanes: Bromodomain-containing protein 9 antibody at 2μg/ml + Mouse liver tissue
    Secondary
    Goat polyclonal to rabbit IgG at 1/10000 dilution
    Predicted band size: 68, 23, 54, 27, 56, 61 kDa
    Observed band size: 68 kDa

  • Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA888000ESR2HU at dilution of 1:100

  • Immunofluorescent analysis of HepG2 cells using CSB-PA888000ESR2HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

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Product Details

Full Product Name
Rabbit anti-Homo sapiens (Human) BRD9 Polyclonal antibody
Uniprot No.
Target Names
BRD9
Alternative Names
BRD9 antibody; UNQ3040/PRO9856Bromodomain-containing protein 9 antibody; Rhabdomyosarcoma antigen MU-RMS-40.8 antibody
Raised in
Rabbit
Species Reactivity
Human, Mouse
Immunogen
Recombinant Human Bromodomain-containing protein 9 protein (338-597AA)
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Form
Liquid
Tested Applications
ELISA, WB, IHC, IF
Recommended Dilution
Application Recommended Dilution
WB 1:200-1:1000
IHC 1:20-1:200
IF 1:50-1:200
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Description

A partial recombinant protein of the human BRD9 protein, corresponding to the 338-597 amino acids, was used to immunize the rabbit to synthesize the BRD9 antibody. The resulting BRD9 antibody is an unconjugated IgG isoform that has undergone antigen affinity purification.

To ensure its reliability, this BRD9 polyclonal antibody has been rigorously quality controlled in multiple applications, including ELISA, WB, IHC, and IF. It has been shown to be reactive with human and mouse BRD9 proteins. The BRD9 protein is a subunit of the BAF chromatin remodeling complex, which plays a crucial role in regulating gene expression and chromatin structure. Additionally, BRD9 has been implicated in other cellular processes, such as cell division, DNA damage repair, and cell differentiation.

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Target Background

Function
Plays a role in chromatin remodeling and regulation of transcription. Acts as a chromatin reader that recognizes and binds acylated histones: binds histones that are acetylated and/or butyrylated. Component of SWI/SNF chromatin remodeling subcomplex GBAF that carries out key enzymatic activities, changing chromatin structure by altering DNA-histone contacts within a nucleosome in an ATP-dependent manner. Orchestrates also the RAD51-RAD54 complex formation and thereby plays a role in homologous recombination (HR).
Gene References into Functions
  1. The bromodomains of BRD9, CECR2, and the second bromodomain of TAF1 recognize the longer butyryl mark on histones. In addition, the TAF1 second bromodomain is capable of binding crotonyl marks. PMID: 26365797
Database Links

HGNC: 25818

KEGG: hsa:65980

STRING: 9606.ENSP00000419765

UniGene: Hs.449278

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