BRD9 Antibody

Datasheet

Code	CSB-PA888000ESR2HU
Size	$297
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Image	Western Blot Positive WB detected in: 786-O whole cell lysate(30µg), THP-1 whole cell lysate(30µg), MCF7 whole cell lysate(30µg),Mouse Liver tissue lysate(20µg) All lanes: BRD9 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 67,23,54,27,56,61 kDa Observed band size: 67 kDa Exposure time: 120s IHC image of CSB-PA888000ESR2HU diluted at 1:100 and staining in paraffin-embedded human brain performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody
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Product Details
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) BRD9 Polyclonal antibody

Uniprot No.

Q9H8M2

Target Names

BRD9

Alternative Names

BRD9 antibody; UNQ3040/PRO9856Bromodomain-containing protein 9 antibody; Rhabdomyosarcoma antigen MU-RMS-40.8 antibody

Raised in

Rabbit

Species Reactivity

Human, Mouse

Immunogen

Synthetic protein corresponding to residues near the C terminal of human BRD9

Immunogen Species

Homo sapiens (Human)

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IHC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
IHC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

A partial recombinant protein of the human BRD9 protein, corresponding to the 338-597 amino acids, was used to immunize the rabbit to synthesize the BRD9 antibody. The resulting BRD9 antibody is an unconjugated IgG isoform that has undergone antigen affinity purification.

To ensure its reliability, this BRD9 polyclonal antibody has been rigorously quality controlled in multiple applications, including ELISA, WB, IHC, and IF. It has been shown to be reactive with human and mouse BRD9 proteins. The BRD9 protein is a subunit of the BAF chromatin remodeling complex, which plays a crucial role in regulating gene expression and chromatin structure. Additionally, BRD9 has been implicated in other cellular processes, such as cell division, DNA damage repair, and cell differentiation.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Plays a role in chromatin remodeling and regulation of transcription. Acts as a chromatin reader that recognizes and binds acylated histones: binds histones that are acetylated and/or butyrylated. Component of SWI/SNF chromatin remodeling subcomplex GBAF that carries out key enzymatic activities, changing chromatin structure by altering DNA-histone contacts within a nucleosome in an ATP-dependent manner. Orchestrates also the RAD51-RAD54 complex formation and thereby plays a role in homologous recombination (HR).

Gene References into Functions

The bromodomains of BRD9, CECR2, and the second bromodomain of TAF1 recognize the longer butyryl mark on histones. In addition, the TAF1 second bromodomain is capable of binding crotonyl marks. PMID: 26365797

Database Links

HGNC: 25818

KEGG: hsa:65980

STRING: 9606.ENSP00000419765

UniGene: Hs.449278

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Email: [email protected]
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Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Related pathways

Target Therapy

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

No. 269, Shendun 5th Road, Donghu Hi-Tech Development Area, Hubei Province, 430206, P.R.China

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