BUD13 Antibody

Datasheet

Code	CSB-PA863104LA01HU
Size	US$166
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Image	IHC image of CSB-PA863104LA01HU diluted at 1:500 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunofluorescence staining of U251 cells with CSB-PA863104LA01HU at 1:250, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) BUD13 Polyclonal antibody

Uniprot No.

Q9BRD0

Target Names

BUD13

Alternative Names

Bud13 antibody; BUD13 homolog (S. cerevisiae) antibody; BUD13 homolog antibody; BUD13_HUMAN antibody; Cwc26 antibody; FLJ27090 antibody; fSAP71 antibody; Functional spliceosome-associated protein 71 antibody; MGC13125 antibody; OTTHUMP00000042639 antibody; OTTHUMP00000042640 antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human BUD13 homolog protein (132-233AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The BUD13 Antibody (Product code: CSB-PA863104LA01HU) is Non-conjugated. For BUD13 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA863104LB01HU	BUD13 Antibody, HRP conjugated	ELISA
FITC	CSB-PA863104LC01HU	BUD13 Antibody, FITC conjugated
Biotin	CSB-PA863104LD01HU	BUD13 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, IHC, IF

Recommended Dilution

Application	Recommended Dilution
IHC	1:500-1:1000
IF	1:200-1:500

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Customer Reviews and Q&A

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Target Background

Function

Involved in pre-mRNA splicing as component of the activated spliceosome.

Gene References into Functions

Data concluded that novel mutations in BUD13 did not confer risk for MetS in our study population, but these mutations changed the level of metabolic complements. PMID: 28659142
by an integrated analysis of the genotypes and the serum levels of APOA5, BUD13 and triglyceride, we observed that BUD13 was another potential mediator, besides APOA5, of the association between rs651821 and serum triglyceride. rs671 (ALDH2), an east Asian-specific common variant, was found to be associated with MetS (Pcombined = 9.7 x 10(-22) ) in Han Chinese PMID: 28371326
When the inter-dependence between alleles was examined using conditional models, five loci on BUD13, ZNF259, and ApoA5 showed possible independent associations. PMID: 26634697
Single nucleotide polymorphisms (Rs17119975) of BUD13 protein did not increase the risk of CHD in the Chinese population. PMID: 26397108
Common SNPs within the BUD13-APOA5 gene region can effect triglyceride and LDL cholesterol response to statin therapy. PMID: 25900265
Single nucleotide polymorphism bud13 gene is associated with hyperlipidaemia. PMID: 24780069
Significant linkage disequilibria were noted among ZNF259, BUD13 and MLXIPL SNPs and serum lipid levels. PMID: 24989072

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Subcellular Location

Nucleus.

Protein Families

CWC26 family

Database Links

HGNC: 28199

KEGG: hsa:84811

STRING: 9606.ENSP00000260210

UniGene: Hs.437341

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Service

Phage Display Service

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Protein Service

Gene Synthesis Service

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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