CASP12 Antibody

Datasheet

Code	CSB-PA747641LA01HU
Size	US$166
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Image	Western Blot Positive WB detected in: Mouse brain tissue All lanes: CASP12 antibody at 2.7μg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 39, 20, 30 kDa Observed band size: 39 kDa Immunohistochemistry of paraffin-embedded human liver tissue using CSB-PA747641LA01HU at dilution of 1:100 Immunofluorescent analysis of Hela cells using CSB-PA747641LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Product Details
Citations
Related Products
Customer Reviews and Q&A
Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) CASP12 Polyclonal antibody

Uniprot No.

Q6UXS9

Target Names

CASP12

Alternative Names

CASP 12 antibody; CASP-12 antibody; Casp12 antibody; CASP12P1 antibody; caspase 12 (gene/pseudogene) antibody; caspase 12 pseudogene 1 antibody; CASPC_HUMAN antibody; Inactive caspase-12 antibody; OTTHUMP00000207032 antibody

Raised in

Rabbit

Species Reactivity

Human, Mouse

Immunogen

Recombinant Human Inactive caspase-12 protein (61-282AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The CASP12 Antibody (Product code: CSB-PA747641LA01HU) is Non-conjugated. For CASP12 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA747641LB01HU	CASP12 Antibody, HRP conjugated	ELISA
FITC	CSB-PA747641LC01HU	CASP12 Antibody, FITC conjugated
Biotin	CSB-PA747641LD01HU	CASP12 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:20-1:200
IF	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Citations

A study on ER stress-induced apoptosis pathway in cervical cancer HeLa cells treated with biosynthesized gold nanoparticles MR Kamala Priya,Bulletin of the National Research Centre,2021
Glutathione Peroxidase 8 Suppression by Histone Deacetylase Inhibitors Enhances Endoplasmic Reticulum Stress and Cell Death by Oxidative Stress in Hepatocellular Carcinoma Cells HA Lee,Antioxidants,2021
Apoptotic Activity in Cervical Cancer HeLa Cell Lines Treated with Chitosan Nanoconjugated Drug Doxorubicin - as Nanocarrier for Drug Delivery Priya M R K, et al,/,2019

Customer Reviews and Q&A

■ Customer Reviews

Average Rating:

5.0 - 2 reviews

Submit a Review here

Applications : Western blot assays

Sample type: Human cells

Review: Cleavage of caspase 12 was detected by Western blot. Combined treatment with tBHP and HDACi induced apoptosis in HCC cells. Apoptosis of HCC cells after treatment with tBHP (25 μmol/L), SAHA (1 mol/L), MS275 (1 μmol/L), or combinatorial treatment with tBHP and HDACi.

By Anonymous

Applications : Western blots

Sample type: cells

Review: Expression of apoptotic proteins with respective positive bands at 38 kDa for Caspase 12, 38 kDa for Mcl 1, 26 kDa for BAX, 26 kDa for Bcl 2, 36 kDa for cyt c, 60 kDa for PARP3, Dox—Doxorubicin, S1—NC4, S2—NC5, S3—NC6, S4—NP1, S5—NP6 Dox, S6—Chit-ND1.

By Anonymous

Target Background

Function

Has no protease activity. May reduce cytokine release in response to bacterial lipopolysaccharide during infections. Reduces activation of NF-kappa-B in response to TNF.

Gene References into Functions

Piezo1 plays an important role in the apoptosis of human osteoarthritis-derived chondrocytes through a caspase-12-dependent pathway. PMID: 28731145
study indicated the potential effect of Casp12 was on the activation of NF-kappaB by the degradation of IkappaBalpha in NPC cells. PMID: 28380444
CASP12 genotype thus does not influence the phenotype of systemic lupus erythematosus in African-Americans PMID: 26973091
Endoplasmic reticulum stress may be associated with apoptosis of LECs, resulting in cataract formation in diabetic patients. PMID: 27130368
Endoplasmic reticulum stress related factor ATF6 and caspase-12 trigger apoptosis in neonatal hypoxic-ischemic encephalopathy. PMID: 26261584
Expression of caspase-12 was linked to decreased systemic and adipose tissue inflammation in a cohort of African American obese children. PMID: 26582949
We found that RNA interference-induced Caspase-12 silencing increased NOD1, hBD1 and hBD2 expression PMID: 25503380
conclude that the functional CASP12L allele is not a marker for susceptibility and/or severity of CAP PMID: 24586588
EtOH-induced activation of caspase-12 could be one of the underlying mechanisms of hepatocyte apoptosis. PMID: 24224909
Distribution of CASP12 alleles (#rs497116) in African-Americans (AA) with rheumatoid arthritis (RA). There was no difference in the overall distribution of genotypes within AA with RA, but homozygous patients had lower baseline joint-narrowing scores. PMID: 24515649
CASPASE-12 does not influence the susceptibility to Candida sepsis, nor has any effect on the serum cytokine concentrations in Candida sepsis patients during the course of infection. PMID: 21706251
examines the role that CASP12 plays in down-regulating inflammation, we hypothesize that pathogens which exploit the inflammatory response are restrained by an active CASP12 gene product PMID: 21872999
caspase 12 has acquired deleterious mutations PMID: 12054529
Pathways involving this enzyme are involved in a neurodegenerative disease in vivo, and thus offer novel potential targets for the treatment of prion disorders. PMID: 14532116
Na/Ca exchanger overexpression activates caspase-12 PMID: 15033764
Csp12-L attenuates the inflammatory and innate immune response to endotoxins and in doing so may constitute a risk factor for developing sepsis PMID: 15129283
Observational study of genotype prevalence. (HuGE Navigator) PMID: 16917906
The activation of caspase-9 and depolarization of mitochondrial membrane potential were induced by BIK, which were decreased concomitant with caspase-12 silenced. PMID: 17574210
proteolytic activity of caspase-12 is confined to its own proenzyme PMID: 18332441
hRPE cells express a high level of caspase-12S. The regulated expression of caspase-12S suggests that this caspase recruitment domain (CARD)-only protein may be an endogenous dominant negative regulator that modulates inflammatory responses in hRPE cells. PMID: 18791174
estrogen-mediated inhibition of Csp-12L expression is a built-in mechanism that has evolved to protect females from Listeria monocytogenes infection. PMID: 19447924
the NOD2/RIP2 pathway has a role in recognition of Yersinia, but caspase-12 does not modulate innate host defense against Y. pestis PMID: 19721713
The calcium-calpain-caspase-12-caspase-3 cascade is altered in F508del-CFTR expressing cells. PMID: 20041182
Reactive oxygen species promote caspase-12 expression and tubular apoptosis in diabetic nephropathy. PMID: 20299359
Observational study of gene-disease association. (HuGE Navigator) PMID: 20299368
Observational study of gene-disease association. (HuGE Navigator) PMID: 20423816
Observational study of gene-disease association. (HuGE Navigator) PMID: 20577092
Constitutively active forms of caspase-12 (rev-Deltapro1 and rev-Deltapro2) could induce cell death in cells transfected with the corresponding expression vectors. PMID: 20646990

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Protein Families

Peptidase C14A family

Tissue Specificity

Detected in heart, kidney, liver, lung, pancreas, small intestine, spleen, stomach, thymus and testis.

Database Links

HGNC: 19004

OMIM: 608633

KEGG: hsa:100506742

STRING: 9606.ENSP00000424038

UniGene: Hs.476989

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Related pathways

Apoptosis Protein processing in endoplasmic reticulum NOD-like receptor signaling pathway ER Stress pathway

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