CASP12 Antibody

Code CSB-PA747641LA01HU
Size US$299Purchase it in Cusabio online store
(only available for customers from the US)
Image
  • Western Blot
    Positive WB detected in: Mouse brain tissue
    All lanes: CASP12 antibody at 2.7μg/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 39, 20, 30 kDa
    Observed band size: 39 kDa

  • Immunohistochemistry of paraffin-embedded human liver tissue using CSB-PA747641LA01HU at dilution of 1:100

  • Immunofluorescent analysis of Hela cells using CSB-PA747641LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

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Product Details

Full Product Name Rabbit anti-Homo sapiens (Human) CASP12 Polyclonal antibody
Uniprot No. Q6UXS9
Target Names CASP12
Alternative Names CASP 12 antibody; CASP-12 antibody; Casp12 antibody; CASP12P1 antibody; caspase 12 (gene/pseudogene) antibody; caspase 12 pseudogene 1 antibody; CASPC_HUMAN antibody; Inactive caspase-12 antibody; OTTHUMP00000207032 antibody
Raised in Rabbit
Species Reactivity Human, Mouse
Immunogen Recombinant Human Inactive caspase-12 protein (61-282AA)
Immunogen Species Homo sapiens (Human)
Conjugate Non-conjugated
Clonality Polyclonal
Isotype IgG
Purification Method >95%, Protein G purified
Concentration It differs from different batches. Please contact us to confirm it.
Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form Liquid
Tested Applications ELISA, WB, IHC, IF
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:5000
IHC 1:20-1:200
IF 1:50-1:200
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Applications : Western blot assays

Sample type: Human cells

Review: Cleavage of caspase 12 was detected by Western blot. Combined treatment with tBHP and HDACi induced apoptosis in HCC cells. Apoptosis of HCC cells after treatment with tBHP (25 μmol/L), SAHA (1 mol/L), MS275 (1 μmol/L), or combinatorial treatment with tBHP and HDACi.

By Anonymous

Applications : Western blots

Sample type: cells

Review: Expression of apoptotic proteins with respective positive bands at 38 kDa for Caspase 12, 38 kDa for Mcl 1, 26 kDa for BAX, 26 kDa for Bcl 2, 36 kDa for cyt c, 60 kDa for PARP3, Dox—Doxorubicin, S1—NC4, S2—NC5, S3—NC6, S4—NP1, S5—NP6 Dox, S6—Chit-ND1.

By Anonymous

Target Background

Function
(From Uniprot)
Has no protease activity. May reduce cytokine release in response to bacterial lipopolysaccharide during infections. Reduces activation of NF-kappa-B in response to TNF.
Gene References into Functions
  1. Piezo1 plays an important role in the apoptosis of human osteoarthritis-derived chondrocytes through a caspase-12-dependent pathway. PMID: 28731145
  2. study indicated the potential effect of Casp12 was on the activation of NF-kappaB by the degradation of IkappaBalpha in NPC cells. PMID: 28380444
  3. CASP12 genotype thus does not influence the phenotype of systemic lupus erythematosus in African-Americans PMID: 26973091
  4. Endoplasmic reticulum stress may be associated with apoptosis of LECs, resulting in cataract formation in diabetic patients. PMID: 27130368
  5. Endoplasmic reticulum stress related factor ATF6 and caspase-12 trigger apoptosis in neonatal hypoxic-ischemic encephalopathy. PMID: 26261584
  6. Expression of caspase-12 was linked to decreased systemic and adipose tissue inflammation in a cohort of African American obese children. PMID: 26582949
  7. We found that RNA interference-induced Caspase-12 silencing increased NOD1, hBD1 and hBD2 expression PMID: 25503380
  8. conclude that the functional CASP12L allele is not a marker for susceptibility and/or severity of CAP PMID: 24586588
  9. EtOH-induced activation of caspase-12 could be one of the underlying mechanisms of hepatocyte apoptosis. PMID: 24224909
  10. Distribution of CASP12 alleles (#rs497116) in African-Americans (AA) with rheumatoid arthritis (RA). There was no difference in the overall distribution of genotypes within AA with RA, but homozygous patients had lower baseline joint-narrowing scores. PMID: 24515649
  11. CASPASE-12 does not influence the susceptibility to Candida sepsis, nor has any effect on the serum cytokine concentrations in Candida sepsis patients during the course of infection. PMID: 21706251
  12. examines the role that CASP12 plays in down-regulating inflammation, we hypothesize that pathogens which exploit the inflammatory response are restrained by an active CASP12 gene product PMID: 21872999
  13. caspase 12 has acquired deleterious mutations PMID: 12054529
  14. Pathways involving this enzyme are involved in a neurodegenerative disease in vivo, and thus offer novel potential targets for the treatment of prion disorders. PMID: 14532116
  15. Na/Ca exchanger overexpression activates caspase-12 PMID: 15033764
  16. Csp12-L attenuates the inflammatory and innate immune response to endotoxins and in doing so may constitute a risk factor for developing sepsis PMID: 15129283
  17. Observational study of genotype prevalence. (HuGE Navigator) PMID: 16917906
  18. The activation of caspase-9 and depolarization of mitochondrial membrane potential were induced by BIK, which were decreased concomitant with caspase-12 silenced. PMID: 17574210
  19. proteolytic activity of caspase-12 is confined to its own proenzyme PMID: 18332441
  20. hRPE cells express a high level of caspase-12S. The regulated expression of caspase-12S suggests that this caspase recruitment domain (CARD)-only protein may be an endogenous dominant negative regulator that modulates inflammatory responses in hRPE cells. PMID: 18791174
  21. estrogen-mediated inhibition of Csp-12L expression is a built-in mechanism that has evolved to protect females from Listeria monocytogenes infection. PMID: 19447924
  22. the NOD2/RIP2 pathway has a role in recognition of Yersinia, but caspase-12 does not modulate innate host defense against Y. pestis PMID: 19721713
  23. The calcium-calpain-caspase-12-caspase-3 cascade is altered in F508del-CFTR expressing cells. PMID: 20041182
  24. Reactive oxygen species promote caspase-12 expression and tubular apoptosis in diabetic nephropathy. PMID: 20299359
  25. Observational study of gene-disease association. (HuGE Navigator) PMID: 20299368
  26. Observational study of gene-disease association. (HuGE Navigator) PMID: 20423816
  27. Observational study of gene-disease association. (HuGE Navigator) PMID: 20577092
  28. Constitutively active forms of caspase-12 (rev-Deltapro1 and rev-Deltapro2) could induce cell death in cells transfected with the corresponding expression vectors. PMID: 20646990

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Protein Families Peptidase C14A family
Tissue Specificity Detected in heart, kidney, liver, lung, pancreas, small intestine, spleen, stomach, thymus and testis.
Database Links

HGNC: 19004

OMIM: 608633

KEGG: hsa:100506742

STRING: 9606.ENSP00000424038

UniGene: Hs.476989

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