CDC34 Antibody

Code CSB-PA001505
Size US$167
Image
  • Western Blot analysis of COLO205 cells using Cdc34 Polyclonal Antibody
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Product Details

Uniprot No. P49427
Target Names CDC34
Alternative Names Cdc 34 antibody; Cdc34 antibody; Cell division cycle 34 antibody; Cell division cycle 34 homolog (S. cerevisiae) antibody; Cell division cycle 34 homolog antibody; E2 CDC34 antibody; UB2R1_HUMAN antibody; UBC 3 antibody; UBC3 antibody; UBCH3 antibody; UBE2 R1 antibody; UBE2R1 antibody; Ubiquitin carrier protein antibody; Ubiquitin conjugating enzyme Cdc34 antibody; Ubiquitin conjugating enzyme E2 32 kDa complementing antibody; Ubiquitin protein ligase antibody; Ubiquitin protein ligase R1 antibody; Ubiquitin-conjugating enzyme E2 R1 antibody; Ubiquitin-conjugating enzyme E2-32 kDa complementing antibody; Ubiquitin-conjugating enzyme E2-CDC34 antibody; Ubiquitin-protein ligase R1 antibody
Raised in Rabbit
Species Reactivity Human,Mouse,Rat
Immunogen Synthesized peptide derived from the Internal region of Human Cdc34.
Immunogen Species Homo sapiens (Human)
Conjugate Non-conjugated
Isotype IgG
Purification Method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration It differs from different batches. Please contact us to confirm it.
Buffer Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Form Liquid
Tested Applications WB, ELISA
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:2000
ELISA 1:10000
Protocols Western Blotting(WB) Protocol
ELISA Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Data

Function Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-48'-linked polyubiquitination
Gene References into Functions
  1. study finds that mutation of the catalytically active cysteine to serine (C93S) results in the reduced ubiquitination, increased stability, and attenuated degradation rate of CDC34; Through semi-quantitative proteomics, identified the CDC34-interacting proteins and discovered that the wild-type and mutant proteins have many differentially interacted proteins PMID: 29564676
  2. Study shows that Ube2R1/2 forms a salt bridge interaction between a conserved Asp residue on Ube2R1/2 and acceptor ubiquitin residue Arg 54 and that perturbation of this interaction leads to the severe loss of UbeR2 activity. Results also provide new insight into how the Ube2R1/2 acidic loop may participate in catalysis. PMID: 27044868
  3. ubiquitin binding by the acidic loops of Ube2g1 and Ube2r1 enzymes distinguishes their Lys-48-ubiquitylation activities PMID: 25471371
  4. We discuss how these results can explain the rapid association of Cdc34 and SCF. PMID: 25425648
  5. Here we have investigated how the acidic loop in human Cdc34 promotes ubiquitination, identifying two key molecular events during which the acidic loop exerts its influence PMID: 24129577
  6. These data suggest that FBXO15 and Ube2r1 regulate P-gp expression through the ubiquitin-proteasome pathway. PMID: 23465077
  7. Tristetraprolin promotes an increase in expression of mature let-7, which leads to the inhibition of let-7 target gene CDC34 expression and suppresses cell growth. PMID: 22210895
  8. work provides the first structural details that show how the C-terminus of CDC34 might direct a thiolester-bound Ub to control polyubiquitin chain formation PMID: 21296085
  9. the human Cdc34 carboxyl terminus contains a non-covalent ubiquitin binding activity that contributes to SCF-dependent ubiquitination PMID: 20353940
  10. COP9 signalosome protects ubiquitin-conjugating enzyme 3 (UBC3/Cdc34) from beta-transducin repeat-containing protein (betaTrCP)-mediated degradation PMID: 20378537
  11. determined that a ubiquitin (Ub) fused at I kappaB alpha K21 acts as a receptor, directing Cdc34 for rapid and efficient K48-linked Ub chain synthesis that depends on SCF(beta TrCP2) and the substrate's N terminus. PMID: 20347421
  12. degraded by HSV-1 ICP0 while stabilizing cyclins D1 and D3 PMID: 14645576
  13. antisense oligonucleotides targeting the human ubiquitin-conjugating enzyme Cdc34 downregulate its expression, inhibit the degradation of p27Kip1, and prevent cellular proliferation PMID: 15652359
  14. although a Cdc34 mutant bearing a deletion of the C-terminal 36 amino acids (Cdc34 1-200) was efficiently charged with ubiquitin by E1, it was severely reduced for the ability to ubiquitinate p27(Kip1) in vitro compared to wildtype Cdc34 PMID: 16123592
  15. Data suggest that the Skp1.CUL1.F-box-mediated polyubiquitination reaction may require the conversion of Cdc34 from an inactive monomer to a highly active dimeric form. PMID: 16210246
  16. in vivo phosphorylation sites on budding yeast Cdc34 (yCdc34; Ser207 and Ser216) and human Cdc34 (hCdc34 Ser203, Ser222 and Ser231) to serine residues in the acidic tail domain, a region that is critical for Cdc34's cell cycle function PMID: 17461777
  17. In tissue culture cells, Uba6 is required for charging a previously uncharacterized Uba6-specific E2 (Use1), whereas Ube1 is required for charging the cell-cycle E2s Cdc34A and Cdc34B PMID: 17597759
  18. These findings support the hypothesis that human Cdc34 S95 and E108/E112 are required to position the donor ubiquitin optimally for catalysis, in a manner that might depend on E2 dimerization. PMID: 17698585
  19. cisplatin increased ATF5 protein expression via preventing its ubiquitin-dependent degradation, which might be associated with its promoting the nucleus-to-cytoplasm translocation of E2 ubiquitin-conjugating enzyme Cdc34 PMID: 18458088
  20. Cdc34 is a functional target of let-7 and that let-7 induces down-regulation of Cdc34, stabilization of the Wee1 kinase, and an increased fraction of cells in G(2)/M in primary fibroblasts. PMID: 19126550
  21. The acidic tail of the Cdc34 ubiquitin-conjugating enzyme functions in both binding to and catalysis with ubiquitin ligase SCFCdc4. PMID: 19875449
  22. Study shows that chain assembly by ubiquitin ligase SCF and ubiquitin-conjugating enzyme Cdc34 is facilitated by the unusual nature of Cdc34-SCF transactions: Cdc34 binds SCF with nanomolar affinity, nevertheless the complex is extremely dynamic. PMID: 19945379

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Subcellular Location Cytoplasm, Nucleus
Protein Families Ubiquitin-conjugating enzyme family
Tissue Specificity Expressed in testes during spermatogenesis to regulate repression of cAMP-induced transcription.
Database Links

HGNC: 1734

OMIM: 116948

KEGG: hsa:997

STRING: 9606.ENSP00000215574

UniGene: Hs.514997

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