CEP250 Antibody

Code CSB-PA005239GA01HU
Size $600
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Product Details

Uniprot No.
Target Names
CEP250
Alternative Names
CEP250 antibody; CEP2 antibody; CNAP1Centrosome-associated protein CEP250 antibody; 250 kDa centrosomal protein antibody; Cep250 antibody; Centrosomal Nek2-associated protein 1 antibody; C-Nap1 antibody; Centrosomal protein 2 antibody
Raised in
Rabbit
Species Reactivity
Human,Mouse,Rat
Immunogen
Human CEP250,C-NAP1
Immunogen Species
Homo sapiens (Human)
Isotype
IgG
Purification Method
Antigen Affinity purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. -20°C, Avoid freeze / thaw cycles.
Tested Applications
ELISA,WB,IHC,IF
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Background

Function
May be involved in ciliogenesis. Probably plays an important role in centrosome cohesion during interphase.
Gene References into Functions
  1. C-NAP1-null cells were viable and had an increased frequency of premature centriole separation, accompanied by reduced density of the centriolar satellites, with reexpression of C-NAP1 rescuing both phenotypes. Centrosome amplification induced by DNA damage or by PLK4 or CDK2 overexpression was markedly reduced in the absence of C-NAP1. PMID: 28100636
  2. ASPP1/2 interacted with centrosome linker protein C-Nap1. Co-depletion of ASPP1 and ASPP2 inhibited re-association of C-Nap1 with centrosome at the end of mitosis. PMID: 25660448
  3. multisite phosphorylation precipitates centrosome disjunction at the onset of mitosis PMID: 24695856
  4. A homozygous nonsense CEP250 mutation, in combination with a heterozygous C2orf71 nonsense mutation, causes an atypical form of Usher syndrome, characterised by early-onset sensorineural hearing loss and a relatively mild retinitis pigmentosa. PMID: 24780881
  5. Centlein complexes with C-Nap1 and Cep68 at the proximal ends of centrioles during interphase. PMID: 24554434
  6. C-NAP1 and rootletin restrain DNA damage-induced centriole splitting and facilitate ciliogenesis. PMID: 23070519
  7. Data show that the dissociation of C-Nap1 from mitotic centrosomes is regulated by localized phosphorylation rather than generalized proteolysis. PMID: 12140259
  8. CEP135 acts as a platform protein for C-NAP1 at the centriole. PMID: 18851962

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Subcellular Location
Cytoplasm, perinuclear region. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, cilium basal body. Cell projection, cilium, photoreceptor outer segment. Photoreceptor inner segment. Note=Component of the core centrosome. In interphase cells, it specifically associates with the proximal ends of both mother and daughter centrioles. Associates with the centrosome in interphase cells. In mitotic cells, it dissociates from the mitotic spindle poles. At the end of cell division, it reaccumulates at centrosomes.
Tissue Specificity
Ubiquitously and weakly expressed.
Database Links

HGNC: 1859

OMIM: 609689

KEGG: hsa:11190

STRING: 9606.ENSP00000380661

UniGene: Hs.443976

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