CERS5 Antibody

Datasheet

Code	CSB-PA847636LA01HU
Size	US$166
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Image	Western Blot Positive WB detected in: MCF-7 whole cell lysate All lanes: CERS5 antibody at 3µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 46, 40 kDa Observed band size: 46 kDa Immunohistochemistry of paraffin-embedded human melanoma using CSB-PA847636LA01HU at dilution of 1:100 Immunofluorescent analysis of HepG2 cells using CSB-PA847636LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Product Details
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) CERS5 Polyclonal antibody

Uniprot No.

Q8N5B7

Target Names

CERS5

Alternative Names

CERS5; LASS5; Ceramide synthase 5; CerS5; LAG1 longevity assurance homolog 5; Sphingoid base N-palmitoyltransferase CERS5; Sphingosine N-acyltransferase CERS5

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Ceramide synthase 5 protein (332-392AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The CERS5 Antibody (Product code: CSB-PA847636LA01HU) is Non-conjugated. For CERS5 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA847636LB01HU	CERS5 Antibody, HRP conjugated	ELISA
FITC	CSB-PA847636LC01HU	CERS5 Antibody, FITC conjugated
Biotin	CSB-PA847636LD01HU	CERS5 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:20-1:200
IF	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Customer Reviews and Q&A

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Target Background

Function

Ceramide synthase that catalyzes the transfer of the acyl chain from acyl-CoA to a sphingoid base, with high selectivity toward palmitoyl-CoA (hexadecanoyl-CoA; C16:0-CoA)(PubMed:16951403, PubMed:18541923, PubMed:22144673, PubMed:22661289, PubMed:23530041, PubMed:26887952, PubMed:29632068, PubMed:31916624). Can use other acyl donors, but with less efficiency. N-acylates sphinganine and sphingosine bases to form dihydroceramides and ceramides in de novo synthesis and salvage pathways, respectively. Plays a role in de novo ceramide synthesis and surfactant homeostasis in pulmonary epithelia.

Gene References into Functions

LASS5 interacts with SDHB and synergistically represses p53 and p21 activity. PMID: 27280497
Studied the role of ceramide synthase 5 (LASS5 or CERS5) gene in atherosclerosis. Downregulation of LASS5 by siRNA was found to attenuate ceramide production and increase expression of some AMPK target genes in HUVEC. Found that LASS5 was involved in negative regulation of atherosclerosis-related genes, such as AMPK-alpha. PMID: 28424433
mRNA level of CerS5 is higher in cancerous cell lines and decreases following the induction of apoptosis. PMID: 25779024
Overexpression of CerS5 increased apoptosis in HeLa cells. PMID: 20406683
study identifies LASS5 as a genuine dihydroceramide synthase and demonstrates that mammalian dihydroceramide synthases do not require additional subunits for their activity PMID: 16100120
accumulation of C(16)-ceramide in mitochondria formed from the protein kinase C-dependent salvage pathway results at least in part from the action of longevity-assurance homologue 5, and the generated ceramide modulates the p38 cascade via PP1 PMID: 17030510
Down-regulation of either acid sphingomyelinase or LASS 5-attenuated ceramide accumulation and H/R-induced Bax translocation to mitochondria. PMID: 18676372

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Subcellular Location

Endoplasmic reticulum membrane; Multi-pass membrane protein.

Database Links

HGNC: 23749

OMIM: 615335

KEGG: hsa:91012

STRING: 9606.ENSP00000325485

UniGene: Hs.270525

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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