CHD1L Antibody

Datasheet
Code CSB-PA803136LA01HU
See More Details Free Antibody trial simple
Size US$299
Uniprot No. Q86WJ1
Image
  • Western Blot
    Positive WB detected in: HepG2 whole cell lysate, Hela whole cell lysate, A549 whole cell lysate
    All lanes: CHD1L antibody at 3µg/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 101, 91, 78, 89, 70 kDa
    Observed band size: 91 kDa

  • Immunofluorescence staining of HepG2 cells with CSB-PA803136LA01HU at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunofluorescence (IF) Protocol
Immunogen Recombinant Human Chromodomain-helicase-DNA-binding protein 1-like protein (704-897AA)
Raised in Rabbit
Species Reactivity Human
Tested Applications ELISA, WB, IF; Recommended dilution: WB:1:500-1:5000, IF:1:200-1:500
Relevance DNA helicase which plays a role in chromatin-remodeling following DNA damage. Targeted to sites of DNA damage through interaction with poly(ADP-ribose) and functions to regulate chromatin during DNA repair. Able to catalyze nucleosome sliding in an ATP-dependent manner. Helicase activity is strongly stimulated upon poly(ADP-ribose)-binding.
Form Liquid
Conjugate Non-conjugated
Storage Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Purification Method >95%, Protein G purified
Isotype IgG
Clonality Polyclonal
Alias Chromodomain-helicase-DNA-binding protein 1-like (EC 3.6.4.12) (Amplified in liver cancer protein 1), CHD1L, ALC1
Immunogen Species Human
Research Area Epigenetics and Nuclear Signaling
Target Names CHD1L
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Still Have Questions? Leave a Message or Start an on-line Chat
Function DNA helicase which plays a role in chromatin-remodeling following DNA damage. Targeted to sites of DNA damage through interaction with poly(ADP-ribose) and functions to regulate chromatin during DNA repair. Able to catalyze nucleosome sliding in an ATP-dependent manner. Helicase activity is strongly stimulated upon poly(ADP-ribose)-binding.
Subcellular Location Nucleus
Protein Families SNF2/RAD54 helicase family
Tissue Specificity Frequently overexpressed in hepatomacellular carcinomas.
Database Links

HGNC: 1916

OMIM: 613039

KEGG: hsa:9557

STRING: 9606.ENSP00000358262

UniGene: Hs.191164

Related Products

CHD1L Antibodies

CHD1L Antibodies for Homo sapiens (Human)

CHD1L Proteins

CHD1L Proteins for Homo sapiens (Human)

Most popular with customers

Newsletters

Get all the latest information on Events, Sales and Offers. Sign up for newsletter today.

Copyright © 2007-2018 www.cusabio.com CUSABIO TECHNOLOGY LLC All Rights Reserved.