CHD1L Antibody

Datasheet
Code CSB-PA803136LA01HU
See More Details Free Antibody trial simple
Size US$299
Image
  • Western Blot
    Positive WB detected in: HepG2 whole cell lysate, Hela whole cell lysate, A549 whole cell lysate
    All lanes: CHD1L antibody at 3µg/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 101, 91, 78, 89, 70 kDa
    Observed band size: 91 kDa

  • Immunofluorescence staining of HepG2 cells with CSB-PA803136LA01HU at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Product Details

Full Product Name Rabbit anti-Human CHD1L Polyclonal antibody
Uniprot No. Q86WJ1
Target Names CHD1L
Alternative Names Chromodomain-helicase-DNA-binding protein 1-like (EC 3.6.4.12) (Amplified in liver cancer protein 1), CHD1L, ALC1
Raised in Rabbit
Species Reactivity Human
Immunogen Recombinant Human Chromodomain-helicase-DNA-binding protein 1-like protein (704-897AA)
Immunogen Species Human
Conjugate Non-conjugated
Clonality Polyclonal
Isotype IgG
Purification Method >95%, Protein G purified
Concentration It differs from different batches. Please contact us to confirm it.
Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form Liquid
Tested Applications ELISA, WB, IF
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:5000
IF 1:200-1:500
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunofluorescence (IF) Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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Target Data

Function DNA helicase which plays a role in chromatin-remodeling following DNA damage. Targeted to sites of DNA damage through interaction with poly(ADP-ribose) and functions to regulate chromatin during DNA repair. Able to catalyze nucleosome sliding in an ATP-dependent manner. Helicase activity is strongly stimulated upon poly(ADP-ribose)-binding.
Subcellular Location Nucleus
Protein Families SNF2/RAD54 helicase family
Tissue Specificity Frequently overexpressed in hepatomacellular carcinomas.
Database Links

HGNC: 1916

OMIM: 613039

KEGG: hsa:9557

STRING: 9606.ENSP00000358262

UniGene: Hs.191164

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