CHTOP Antibody

Code CSB-PA897109HA01HU
Size US$166
Order now
Image
  • Western blot
    All lanes: CHTOP antibody at 14 μg/ml
    Lane 1: Mouse heart tissue
    Lane 2: Mouse kidney tissue
    Secondary
    Goat polyclonal to rabbit IgG at 1/10000 dilution
    Predicted band size: 27, 22 kDa
    Observed band size: 27, 52 kDa

  • Immunohistochemistry of paraffin-embedded human endometrial cancer using CSB-PA897109HA01HU at dilution of 1:100

  • Immunohistochemistry of paraffin-embedded human glioma using CSB-PA897109HA01HU at dilution of 1:100

The Latest Promotion Free Antibody trial simple
Have Questions? Leave a Message or Start an on-line Chat

Product Details

Full Product Name
Rabbit anti-Homo sapiens (Human) CHTOP Polyclonal antibody
Uniprot No.
Target Names
CHTOP
Alternative Names
C1orf77 antibody; chromatin target of chromatin target of PRMT1 protein antibody; Chromatin target of PRMT1 antibody; Chromatin target of PRMT1 protein antibody; Chromosome 1 open reading frame 77 antibody; CHTOP antibody; CHTOP_HUMAN antibody; DKFZp547E1010 antibody; FL- RP1-178F15.2 antibody; FL-SRAG antibody; FOP antibody; Friend of PRMT1 antibody; Friend of PRMT1 protein antibody; HT031 antibody; MGC131924 antibody; MGC86949 antibody; OTTHUMP00000035122 antibody; OTTHUMP00000035123 antibody; Pp7704 antibody; RP1 178F15.2 antibody; Small arginine- and glycine-rich protein antibody; Small protein rich in arginine and glycine antibody; SRAG antibody; SRAG-3 antibody; SRAG-5 antibody
Raised in
Rabbit
Species Reactivity
Human, Mouse
Immunogen
Recombinant Human Chromatin target of PRMT1 protein (179-248AA)
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated

The CHTOP Antibody (Product code: CSB-PA897109HA01HU) is Non-conjugated. For CHTOP Antibody with conjugates, please check the following table.

Available Conjugates
Conjugate Product Code Product Name Application
HRP CSB-PA897109HB01HU CHTOP Antibody, HRP conjugated ELISA
FITC CSB-PA897109HC01HU CHTOP Antibody, FITC conjugated
Biotin CSB-PA897109HD01HU CHTOP Antibody, Biotin conjugated ELISA
Clonality
Polyclonal
Isotype
IgG
Purification Method
>95%, Protein G purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Form
Liquid
Tested Applications
ELISA, WB, IHC
Recommended Dilution
Application Recommended Dilution
WB 1:1000-1:5000
IHC 1:20-1:200
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Description

The peptide corresponding to amino acids 179-248 of the recombinant human CHTOP is used to immunize rabbits to produce the anti-CHTOP antibody. The resulting antibody is a polyclonal antibody and occurs as an unconjugated IgG. Its purity is over 95% using protein G purified. It only reacts with human and mouse CHTOP protein, which is involved in the activation of estrogen–receptor target genes, the downregulation of fetal γ-globin during the developmental transition from fetal to adult hemoglobin, and mRNA export. This CHTOP antibody is suitable for the detection of CHTOP protein in ELISA, WB, and IHC applications.

Customer Reviews and Q&A

 Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Target Background

Function
Plays an important role in the ligand-dependent activation of estrogen receptor target genes. May play a role in the silencing of fetal globin genes. Recruits the 5FMC complex to ZNF148, leading to desumoylation of ZNF148 and subsequent transactivation of ZNF148 target genes. Plays an important role in the tumorigenicity of glioblastoma cells. Binds to 5-hydroxymethylcytosine (5hmC) and associates with the methylosome complex containing PRMT1, PRMT5, MEP50 and ERH. The CHTOP-methylosome complex associated with 5hmC is recruited to selective sites on the chromosome, where it methylates H4R3 and activates the transcription of genes involved in glioblastomagenesis.; Required for effective mRNA nuclear export and is a component of the TREX complex which is thought to couple mRNA transcription, processing and nuclear export, and specifically associates with spliced mRNA and not with unspliced pre-mRNA. TREX is recruited to spliced mRNAs by a transcription-independent mechanism, binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing- and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export to the cytoplasm via the TAP/NFX1 pathway. The TREX complex is essential for the export of Kaposi's sarcoma-associated herpesvirus (KSHV) intronless mRNAs and infectious virus production. Stimulates DDX39B ATPase and helicase activities. In cooperation with ALYREF/THOC4 enhances NXF1 RNA binding activity.
Gene References into Functions
  1. the cellular level of Chtop is autoregulated via Int2 retention of its own mRNA, which subsequently undergoes NMD. PMID: 27683223
  2. Results suggest that 5hmC plays a critical role in glioblastomagenesis by recruiting the CHTOP-methylosome complex to selective sites on the chromosome, where it methylates H4R3 and activates the transcription of cancer-related genes. PMID: 25284789
  3. Chtop is a component of the dynamic TREX mRNA export complex that, along with Alyref, activates the ATPase and RNA helicase activities of Uap56. PMID: 23299939
  4. The recently identified chromatin factor Friend of Prmt1 (FOP) is a critical modulator of gamma-globin gene expression. PMID: 20688955
  5. Fop is tightly associated with chromatin, and that it is modified by both asymmetric and symmetric arginine methylation in vivo. Fop plays an important role in the ligand-dependent activation of estrogen receptor target genes, including TFF1 (pS2). PMID: 19858291
  6. The reduction in SRAG protein that occurs in proliferating cells was mapped with inhibitors to the G(2)/M phase of the cell cycle. As expected, the overexpression of SRAG reduced the percentage of cells in the G(2)/M phase and increased cell death. PMID: 19254951

Show More

Hide All

Subcellular Location
Nucleus. Nucleus, nucleolus. Nucleus, nucleoplasm. Nucleus speckle.
Tissue Specificity
Expressed in an erythroid progenitor cell line derived from peripheral blood. Expressed in glioblastoma cells.
Database Links

HGNC: 24511

OMIM: 614206

KEGG: hsa:26097

STRING: 9606.ENSP00000357683

UniGene: Hs.611057

icon of phone
Call us
301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
icon of address
Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
icon of social media
Join us with

Subscribe newsletter

Leave a message

* To protect against spam, please pass the CAPTCHA test below.
CAPTCHA verification
© 2007-2024 CUSABIO TECHNOLOGY LLC All rights reserved. 鄂ICP备15011166号-1
webinars: DT3C facilitates antibody internalization X
Place an order now

I. Product details

*
*
*
*

II. Contact details

*
*

III. Ship To

*
*
*
*
*
*
*

IV. Bill To

*
*
*
*
*
*
*
*