CHTOP Antibody

Datasheet

Code	CSB-PA897109HA01HU
Size	US$166
	Order now
Image	Western blot All lanes: CHTOP antibody at 14 μg/ml Lane 1: Mouse heart tissue Lane 2: Mouse kidney tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 27, 22 kDa Observed band size: 27, 52 kDa Immunohistochemistry of paraffin-embedded human endometrial cancer using CSB-PA897109HA01HU at dilution of 1:100 Immunohistochemistry of paraffin-embedded human glioma using CSB-PA897109HA01HU at dilution of 1:100
Promotion
Have Questions?	Leave a Message or Start an on-line Chat

Product Details
Related Products
Customer Reviews and Q&A
Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) CHTOP Polyclonal antibody

Uniprot No.

Q9Y3Y2

Target Names

CHTOP

Alternative Names

C1orf77 antibody; chromatin target of chromatin target of PRMT1 protein antibody; Chromatin target of PRMT1 antibody; Chromatin target of PRMT1 protein antibody; Chromosome 1 open reading frame 77 antibody; CHTOP antibody; CHTOP_HUMAN antibody; DKFZp547E1010 antibody; FL- RP1-178F15.2 antibody; FL-SRAG antibody; FOP antibody; Friend of PRMT1 antibody; Friend of PRMT1 protein antibody; HT031 antibody; MGC131924 antibody; MGC86949 antibody; OTTHUMP00000035122 antibody; OTTHUMP00000035123 antibody; Pp7704 antibody; RP1 178F15.2 antibody; Small arginine- and glycine-rich protein antibody; Small protein rich in arginine and glycine antibody; SRAG antibody; SRAG-3 antibody; SRAG-5 antibody

Raised in

Rabbit

Species Reactivity

Human, Mouse

Immunogen

Recombinant Human Chromatin target of PRMT1 protein (179-248AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The CHTOP Antibody (Product code: CSB-PA897109HA01HU) is Non-conjugated. For CHTOP Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA897109HB01HU	CHTOP Antibody, HRP conjugated	ELISA
FITC	CSB-PA897109HC01HU	CHTOP Antibody, FITC conjugated
Biotin	CSB-PA897109HD01HU	CHTOP Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC

Recommended Dilution

Application	Recommended Dilution
WB	1:1000-1:5000
IHC	1:20-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

The peptide corresponding to amino acids 179-248 of the recombinant human CHTOP is used to immunize rabbits to produce the anti-CHTOP antibody. The resulting antibody is a polyclonal antibody and occurs as an unconjugated IgG. Its purity is over 95% using protein G purified. It only reacts with human and mouse CHTOP protein, which is involved in the activation of estrogen–receptor target genes, the downregulation of fetal γ-globin during the developmental transition from fetal to adult hemoglobin, and mRNA export. This CHTOP antibody is suitable for the detection of CHTOP protein in ELISA, WB, and IHC applications.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

■ Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Target Background

Function

Plays an important role in the ligand-dependent activation of estrogen receptor target genes. May play a role in the silencing of fetal globin genes. Recruits the 5FMC complex to ZNF148, leading to desumoylation of ZNF148 and subsequent transactivation of ZNF148 target genes. Plays an important role in the tumorigenicity of glioblastoma cells. Binds to 5-hydroxymethylcytosine (5hmC) and associates with the methylosome complex containing PRMT1, PRMT5, MEP50 and ERH. The CHTOP-methylosome complex associated with 5hmC is recruited to selective sites on the chromosome, where it methylates H4R3 and activates the transcription of genes involved in glioblastomagenesis.; Required for effective mRNA nuclear export and is a component of the TREX complex which is thought to couple mRNA transcription, processing and nuclear export, and specifically associates with spliced mRNA and not with unspliced pre-mRNA. TREX is recruited to spliced mRNAs by a transcription-independent mechanism, binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing- and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export to the cytoplasm via the TAP/NFX1 pathway. The TREX complex is essential for the export of Kaposi's sarcoma-associated herpesvirus (KSHV) intronless mRNAs and infectious virus production. Stimulates DDX39B ATPase and helicase activities. In cooperation with ALYREF/THOC4 enhances NXF1 RNA binding activity.

Gene References into Functions

the cellular level of Chtop is autoregulated via Int2 retention of its own mRNA, which subsequently undergoes NMD. PMID: 27683223
Results suggest that 5hmC plays a critical role in glioblastomagenesis by recruiting the CHTOP-methylosome complex to selective sites on the chromosome, where it methylates H4R3 and activates the transcription of cancer-related genes. PMID: 25284789
Chtop is a component of the dynamic TREX mRNA export complex that, along with Alyref, activates the ATPase and RNA helicase activities of Uap56. PMID: 23299939
The recently identified chromatin factor Friend of Prmt1 (FOP) is a critical modulator of gamma-globin gene expression. PMID: 20688955
Fop is tightly associated with chromatin, and that it is modified by both asymmetric and symmetric arginine methylation in vivo. Fop plays an important role in the ligand-dependent activation of estrogen receptor target genes, including TFF1 (pS2). PMID: 19858291
The reduction in SRAG protein that occurs in proliferating cells was mapped with inhibitors to the G(2)/M phase of the cell cycle. As expected, the overexpression of SRAG reduced the percentage of cells in the G(2)/M phase and increased cell death. PMID: 19254951

Hide All

Subcellular Location

Nucleus. Nucleus, nucleolus. Nucleus, nucleoplasm. Nucleus speckle.

Tissue Specificity

Expressed in an erythroid progenitor cell line derived from peripheral blood. Expressed in glioblastoma cells.

Database Links

HGNC: 24511

OMIM: 614206

KEGG: hsa:26097

STRING: 9606.ENSP00000357683

UniGene: Hs.611057

Promotions

CUSABIO Premium Active Proteins 20% OFF SALE

Email: [email protected]
Distributors Worldwide

Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

Join us with

Subscribe newsletter

Leave a message

Message

Product >> Product Name：
Product >> Code：
Name： *
Email： *
Company/Organization：
Phone：
Since emails may get flagged by spam filters occasionally, we recommend leaving your phone number as a precaution. Your information is safe with us.
Question/Comment： *
Your Region： *
I'd like to receive email newsletters from Cusabio

Yes

No
How do you know CUSABIO?

Email

Distributor

Search engine

Advertisement

Others
CAPTCHA verification：
Please enter the characters shown. Click the image to refresh if unclear.

Place an order now

I. Product details

Product name *

Code *

Size *

Quantity *

Message

II. Contact details

Name *

Email address *

Company/Organization

Phone

III. Ship To

Address *

City *

State/Province *

Zip/Post code *

Country *

Attn Name *

Phone# *

IV. Bill To Same as Shipping Address