Product Details

Uniprot No.

O60543

Target Names

CIDEA

Alternative Names

CIDEACell death activator CIDE-A antibody; Cell death-inducing DFFA-like effector A antibody

Raised in

Rabbit

Species Reactivity

Human,Mouse,Rat

Immunogen

Human CIDEA

Immunogen Species

Homo sapiens (Human)

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. -20°C, Avoid freeze / thaw cycles.

Tested Applications

ELISA,WB

Protocols

ELISA Protocol
Western Blotting(WB) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Target Background

Function

Acts as a CEBPB coactivator in mammary epithelial cells to control the expression of a subset of CEBPB downstream target genes, including ID2, IGF1, PRLR, SOCS1, SOCS3, XDH, but not casein. By interacting with CEBPB, strengthens the association of CEBPB with the XDH promoter, increases histone acetylation and dissociates HDAC1 from the promoter. Binds to lipid droplets and regulates their enlargement, thereby restricting lipolysis and favoring storage. At focal contact sites between lipid droplets, promotes directional net neutral lipid transfer from the smaller to larger lipid droplets. The transfer direction may be driven by the internal pressure difference between the contacting lipid droplet pair and occurs at a lower rate than that promoted by CIDEC. When overexpressed, induces apoptosis. The physiological significance of its role in apoptosis is unclear.

Gene References into Functions

Cidea, unexpectedly, functions molecularly as an indirect inhibitor of thermogenesis via inhibition of UCP1 activity. PMID: 27923808
These data establish a functional role for Cidea and suggest that, in humans, the association between Cidea levels in white fat and metabolic health is not only correlative but also causative. PMID: 26118629
Since Cide-A protein plays a role in the development of metabolic diseases such as obesity, metabolic syndrome, type 2 diabetes and their vascular complications, CIDE -A gene and protein are potential therapeutic targets for these diseases. PMID: 24413203
The lowest mean relative of the gene expression for CIDE-A was observed in the group of obese patients with aortic aneurysm and lipid disorders PMID: 25720106
the association of tag-single nucleotide polymorphisms and haplotype structures of the CIDEA gene with obesity in a Han Chinese population, was investigated. PMID: 24057179
CIDE proteins expression correlate with tumor and survival characteristics in patients with renal cell carcinoma. PMID: 23475172
Insulin regulates CIDEA and CIDEC expression via PI3K, and it regulates expression of each protein via Akt1/2-and JNK2-dependent pathways, respectively, in human adipocytes. PMID: 21636835
The proportion of subjects with CIDEA) gene V115F (G/T) polymorphism with phenotypes of metabolic syndrome in a Chinese population was significantly higher based on genotype, in the order: GG PMID: 21106268
CIDEA binds to liver X receptors and regulates their activity in vitro. PMID: 21315073
These data indicate that the carboxy-terminal domain of Cidea directs lipid droplet targeting, lipid droplet clustering, and triglyceride accumulation, whereas the amino terminal domain is required for Cidea-mediated development of enlarged lipid droplets PMID: 20810722
CIDEC is essential for the differentiation of adipose tissue PMID: 20945533
These results suggest that CIDEA and CIDEC are novel genes regulated by insulin in human adipocytes and may play key roles in the effects of insulin, such as anti-apoptosis and lipid droplet formation. PMID: 20154362
Methylation status of CIDEA, HAAO and RXFP3 had significant association with microsatellite instability in endometrial tumors. PMID: 20211485
We propose an important and human-specific role for CIDEA in lipolysis regulation and metabolic complications of obesity. PMID: 15919794
results support a role for cell death-inducing DFFA (DNA fragmentation factor-alpha)-like effector A (CIDEA) alleles in human obesity PMID: 16186410
deglycosylation of CIDE-A correlated with enhanced nuclear export of the protein, and that high level of nonglycosylated CIDE-A inhibited TGFbeta1-dependent cell death. PMID: 17080483
No dysregulation of CIDEA expression was observed in individuals with the metabolic syndrome PMID: 17895319
The expression of the CIDE-A gene was regulated by CpG methylation of the promoter region. PMID: 18033804
F allele of the CIDEA gene may serve as a risk factor for phenotypes related to metabolic syndrome in Japanese men. PMID: 18328351
Cidea and other lipid droplet proteins define a novel, highly regulated pathway of triglyceride deposition in human WAT. PMID: 18509062
CIDEa is sequestered in mitochondria while transfer of this potentially dangerous protein from mitochondria into nucleus intensifies or even initiates apoptosis. PMID: 18645223
CIDEA is involved in adipose tissue loss in cancer cachexia and this may, at least in part, be due to its ability to inactivate PDC, thereby switching substrate oxidation in human fat cells from glucose to FAs. PMID: 19010897

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Involvement in disease

In omental and subcutaneous adipose tissue of obese patients matched for BMI, expression levels correlate with insulin sensitivity. Expression is increased 5-6 fold in the group of patients with high insulin sensitivity, compared to the insulin-resistant group. This observation is consistent with the idea that triglyceride storage in adipocytes plays an important role in sequestering triglycerides and fatty acids away from the circulation and peripheral tissues, thus enhancing insulin sensitivity in liver and muscle.

Subcellular Location

Lipid droplet. Nucleus.

Tissue Specificity

Expressed in omental and subcutaneous adipose tissue (at protein level).

Database Links

HGNC: 1976

OMIM: 604440

KEGG: hsa:1149

STRING: 9606.ENSP00000320209

UniGene: Hs.249129