CLEC10A Antibody

Datasheet

Code	CSB-PA811601LA01HU
Size	US$166
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Image	IHC image of CSB-PA811601LA01HU diluted at 1:100 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunofluorescence staining of HepG2 cells with CSB-PA811601LA01HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) CLEC10A Polyclonal antibody

Uniprot No.

Q8IUN9

Target Names

CLEC10A

Alternative Names

C type (calcium dependent carbohydrate recognition domain) lectin superfamily member 13 (macrophage derived) antibody; C type (calcium dependent carbohydrate recognition domain) lectin superfamily member 14 (macrophage derived) antibody; C type lectin domain family 10; member A antibody; C type lectin superfamily member 14 antibody; C-type lectin domain family 10 member A antibody; C-type lectin superfamily member 14 antibody; CD301 antibody; CD301 antigen antibody; CD301A antibody; CLC10_HUMAN antibody; CLEC10A antibody; CLECSF13 antibody; CLECSF14 antibody; HML antibody; HML2 antibody; Lectin antibody; Macrophage C type lectin antibody; MACROPHAGE GALACTOSE-TYPE C-TYPE antibody; Macrophage lectin 2 (calcium dependent) antibody; Macrophage lectin 2 antibody; MGL antibody; Mgl1 antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human C-type lectin domain family 10 member A protein (66-316AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The CLEC10A Antibody (Product code: CSB-PA811601LA01HU) is Non-conjugated. For CLEC10A Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA811601LB01HU	CLEC10A Antibody, HRP conjugated	ELISA
FITC	CSB-PA811601LC01HU	CLEC10A Antibody, FITC conjugated
Biotin	CSB-PA811601LD01HU	CLEC10A Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, IHC, IF

Recommended Dilution

Application	Recommended Dilution
IHC	1:20-1:200
IF	1:50-1:200

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

Probable role in regulating adaptive and innate immune responses. Binds in a calcium-dependent manner to terminal galactose and N-acetylgalactosamine units, linked to serine or threonine. These sugar moieties are known as Tn-Ag and are expressed in a variety of carcinoma cells.

Gene References into Functions

this paper shows that key gene of intermediate proinflammatory monocytes, such as CLEC10A, is expressed three- to fourfold more in juvenile idiopathic arthritis-enthesitis-related arthritis PMID: 27706807
BRAF(V600E) mutation induces MGL ligand expression, thereby providing a direct link between oncogenic transformation and aberrant expression of immunosuppressive glycans in colorectal neoplasms. PMID: 26172302
These results indicate a role of MGL as an immunomodulator within the tumour microenvironment interfering with Treg functions, suggesting its possible use in the design of anticancer vaccines. PMID: 26147970
expression of GalNAc moieties mirrors the T cell activation status, and thus only highly stimulated T cells are prone to the suppressive action of MGL. PMID: 23918927
MGL triggering synergized with TLR2-induced pathways, leading to elevated IL-10 mRNA levels & enhanced TNF-alpha mRNA stability. It promoted phosphorylation of the ERK & CREB, fine-tuning the DC maturation phenotype. PMID: 23744646
Described is the use of recombinant CLEC10A (CD301), a human glycoreceptor of the C-type lectin family, for the detection of ligands in sections from formalin-fixed, paraffin-embedded normal and cancerous mammary tissues. PMID: 23275449
MGL engagement improved DC performance as antigen-presenting cells, promoting the upregulation of maturation markers, a decrease in phagocytosis, an enhancement of motility, and most importantly an increase in antigen-specific CD8(+) T-cell activation. PMID: 22531918

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Subcellular Location

Membrane; Single-pass type II membrane protein.

Database Links

HGNC: 16916

OMIM: 605999

KEGG: hsa:10462

STRING: 9606.ENSP00000254868

UniGene: Hs.54403

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