CLPB Antibody

Code CSB-PA884428LA01HU
Size US$166
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Image
  • Western blot
    All lanes: CLPB antibody at 5μg/ml + 293T whole cell lysate
    Secondary
    Goat polyclonal to rabbit IgG at 1/10000 dilution
    Predicted band size: 79, 76, 73, 75, 58 kDa
    Observed band size: 79 kDa

  • Immunohistochemistry of paraffin-embedded human adrenal gland tissue using CSB-PA884428LA01HU at dilution of 1:100

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Product Details

Full Product Name
Rabbit anti-Homo sapiens (Human) CLPB Polyclonal antibody
Uniprot No.
Target Names
CLPB
Alternative Names
Caseinolytic peptidase B antibody; Caseinolytic peptidase B protein homolog antibody; clpB antibody; ClpB caseinolytic peptidase B homolog (E. coli) antibody; ClpB caseinolytic peptidase B homolog antibody; CLPB_HUMAN antibody; FLJ13152 antibody; HSP78 antibody; SKD3 antibody; Suppressor of potassium transport defect 3 antibody
Raised in
Rabbit
Species Reactivity
Human
Immunogen
Recombinant Human Caseinolytic peptidase B protein homolog protein (1-350AA)
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated

The CLPB Antibody (Product code: CSB-PA884428LA01HU) is Non-conjugated. For CLPB Antibody with conjugates, please check the following table.

Available Conjugates
Conjugate Product Code Product Name Application
HRP CSB-PA884428LB01HU CLPB Antibody, HRP conjugated ELISA
FITC CSB-PA884428LC01HU CLPB Antibody, FITC conjugated
Biotin CSB-PA884428LD01HU CLPB Antibody, Biotin conjugated ELISA
Clonality
Polyclonal
Isotype
IgG
Purification Method
>95%, Protein G purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Form
Liquid
Tested Applications
ELISA, WB, IHC
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:2000
IHC 1:20-1:200
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Description

Rabbits were immunized to generate the CLPB polyclonal antibody, which is specifically designed to recognize and bind to the human CLPB protein. The development of this antibody involved using a recombinant human CLPB protein that consisted of amino acids 1-350 as an immunogen. The resulting CLPB antibody was then purified using protein G affinity chromatography, achieving a purity level of up to 95%.

This antibody only reacts with human samples. It has been extensively tested in various experimental settings, such as ELISA, WB, and IHC, to investigate the expression, localization, and activity of the CLPB protein in different contexts. These tests have demonstrated the ability of the CLPB polyclonal antibody to effectively identify and detect the CLPB protein in various experimental conditions.

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Target Background

Function
May function as a regulatory ATPase and be related to secretion/protein trafficking process. Involved in mitochondrial-mediated antiviral innate immunity, activates RIG-I-mediated signal transduction and production of IFNB1 and proinflammatory cytokine IL6.
Gene References into Functions
  1. The neonatal presentation of CLPB deficiency predicts the course of disease in later life, which is extremely important for counselling. PMID: 28687938
  2. Case Reports: bi-allelic CLPB mutations cause cataract, renal cysts, nephrocalcinosis and 3-methylglutaconic aciduria, a novel disorder of mitochondrial protein disaggregation. PMID: 25595726
  3. Disruption of CLPB is associated with congenital microcephaly, severe encephalopathy and 3-methylglutaconic aciduria PMID: 25650066
  4. ClpB can passively thread soluble denatured proteins. PMID: 25288401
  5. Mutations in CLPB define a syndrome with intellectual disability, congenital neutropenia, progressive brain atrophy, movement disorder, cataracts, and 3-methylglutaconic aciduria. PMID: 25597510
  6. CLPB is proposed to function as a mitochondrial chaperone involved in disaggregation of misfolded proteins, resulting from stress such as heat denaturation. PMID: 25597511
  7. ClpB-DnaK reactivated all aggregated fusion proteins with similar efficiency, without unfolding native domains, demonstrating that partial threading of the misfolded moiety is sufficient to solubilize aggregates. PMID: 18488042
  8. formation of the DnaK-ClpB bichaperone network is a three step process PMID: 19698713

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Involvement in disease
3-methylglutaconic aciduria with cataracts, neurologic involvement and neutropenia (MEGCANN)
Subcellular Location
Mitochondrion intermembrane space.
Protein Families
ClpA/ClpB family
Tissue Specificity
Widely expressed (at protein level). Expressed in fetal, as well as in adult tissues, with highest levels in adult brain, including thalamus, hippocampus, occipital cortex and parietal cortex. Low expression in granulocytes.
Database Links

HGNC: 30664

OMIM: 616254

KEGG: hsa:81570

STRING: 9606.ENSP00000294053

UniGene: Hs.523877

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