CNNM3 Antibody

Datasheet

Code	CSB-PA843333LA01HU
Size	US$166
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Image	Western Blot Positive WB detected in: MCF-7 whole cell lysate, HepG2 whole cell lysate All lanes: CNNM3 antibody at 5.6µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 77, 71, 74 kDa Observed band size: 48 kDa IHC image of CSB-PA843333LA01HU diluted at 1:200 and staining in paraffin-embedded human heart tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunofluorescence staining of U251 cells with CSB-PA843333LA01HU at 1:66, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Product Details
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) CNNM3 Polyclonal antibody

Uniprot No.

Q8NE01

Target Names

CNNM3

Alternative Names

CNNM3; ACDP3; Metal transporter CNNM3; Ancient conserved domain-containing protein 3; Cyclin-M3

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Metal transporter CNNM3 protein (358-645AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The CNNM3 Antibody (Product code: CSB-PA843333LA01HU) is Non-conjugated. For CNNM3 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA843333LB01HU	CNNM3 Antibody, HRP conjugated	ELISA
FITC	CSB-PA843333LC01HU	CNNM3 Antibody, FITC conjugated
Biotin	CSB-PA843333LD01HU	CNNM3 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:1000-1:5000
IHC	1:200-1:500
IF	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

Probable metal transporter.

Subcellular Location

Cell membrane; Multi-pass membrane protein.

Protein Families

ACDP family

Tissue Specificity

Widely expressed. Expressed at higher level in heart and spleen.

Database Links

HGNC: 104

OMIM: 607804

KEGG: hsa:26505

STRING: 9606.ENSP00000305449

UniGene: Hs.711617

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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