CPNE3 Antibody

Datasheet
Code CSB-PA005902LA01HU
See More Details Free Antibody trial simple
Size US$299
Uniprot No. O75131
Image
  • Western Blot
    Positive WB detected in: HepG2 whole cell lysate
    All lanes: CPNE3 antibody at 2.6µg/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 61 kDa
    Observed band size: 61 kDa

  • IHC image of CSB-PA005902LA01HU diluted at 1:200 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • IHC image of CSB-PA005902LA01HU diluted at 1:200 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • Immunofluorescence staining of HepG2 cells with CSB-PA005902LA01HU at 1:66, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Immunogen Recombinant Human Copine-3 protein (297-442AA)
Raised in Rabbit
Species Reactivity Human
Tested Applications ELISA, WB, IHC, IF; Recommended dilution: WB:1:500-1:5000, IHC:1:200-1:500, IF:1:50-1:200
Relevance Calcium-dependent phospholipid-binding protein that plays a role in ERBB2-mediated tumor cell migration in response to growth factor heregulin stimulation (PubMed:20010870).
Form Liquid
Conjugate Non-conjugated
Storage Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Purification Method >95%, Protein G purified
Isotype IgG
Clonality Polyclonal
Alias Copine-3 (Copine III), CPNE3, CPN3 KIAA0636
Immunogen Species Human
Research Area Signal Transduction
Target Names CPNE3
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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Function Calcium-dependent phospholipid-binding protein that plays a role in ERBB2-mediated tumor cell migration in response to growth factor heregulin stimulation
Subcellular Location Nucleus, Cytoplasm, Cell membrane, Cell junction, Cell junction, focal adhesion
Protein Families Copine family
Tissue Specificity Expressed in breast and weakly in prostate and ovarian tissues (PubMed:20010870). Expressed in neutrophils (at protein level) (PubMed:12949241). Widely expressed (PubMed:11041869). Expressed in the brain. Expressed in neutrophil precursors from the bone m
Database Links

HGNC: 2316

OMIM: 604207

KEGG: hsa:8895

STRING: 9606.ENSP00000198765

UniGene: Hs.191219

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