CRYM Antibody

Datasheet

Code	CSB-PA622791LA01HU
Size	US$166
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Image	IHC image of CSB-PA622791LA01HU diluted at 1:500 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. IHC image of CSB-PA622791LA01HU diluted at 1:500 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunofluorescence staining of A549 cells with CSB-PA622791LA01HU at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) CRYM Polyclonal antibody

Uniprot No.

Q14894

Target Names

CRYM

Alternative Names

CRYM antibody; CRYM_HUMAN antibody; Crystallin mu antibody; DFNA 40 antibody; DFNA40 antibody; Ketimine reductase antibody; Mu crystallin homolog antibody; Mu-crystallin homolog antibody; NADP regulated thyroid hormone binding protein antibody; NADP-regulated thyroid-hormone-binding protein antibody; OTTHUMP00000115878 antibody; THBP antibody; Thiomorpholine carboxylate dehydrogenase antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Ketimine reductase mu-crystallin protein (169-258AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The CRYM Antibody (Product code: CSB-PA622791LA01HU) is Non-conjugated. For CRYM Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA622791LB01HU	CRYM Antibody, HRP conjugated	ELISA
FITC	CSB-PA622791LC01HU	CRYM Antibody, FITC conjugated
Biotin	CSB-PA622791LD01HU	CRYM Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, IHC, IF

Recommended Dilution

Application	Recommended Dilution
IHC	1:500-1:1000
IF	1:50-1:200

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Customer Reviews and Q&A

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Target Background

Function

Specifically catalyzes the reduction of imine bonds in brain substrates that may include cystathionine ketimine (CysK) and lanthionine ketimine (LK). Binds thyroid hormone which is a strong reversible inhibitor. Presumably involved in the regulation of the free intracellular concentration of triiodothyronine and access to its nuclear receptors.

Gene References into Functions

Results suggest that mu-crystallin may play roles in development of cortical and hippocampal pyramidal cells in the early postnatal period, and in the later period, performs cell-specific functions in selected neuronal populations. PMID: 29603402
These findings confirm that, mechanistically, ketimine reductase/CRYM acts as a classical imine reductase and may explain the finding of bound pyruvate in the crystallized protein. PMID: 26173510
ketimine reductase is a key enzyme in the pipecolate pathway, which is the main lysine degradation pathway in the brain PMID: 25931162
The expression of mu-crystallin was regulated through the AP-1 site in the promoter. PMID: 23508717
The purified human mu-crystallin was confirmed to have ketimine reductase activity with a maximum specific activity similar to that of native ovine ketimine reductase PMID: 21332720
Its enzyme function has been determined as a ketimine reductase in mammalian brain. PMID: 21332720
Its ketimine reductase activity is strongly inhibited by thyroid hormones. PMID: 21332720
This protein has been found differentially expressed in thalami from patients with schizophrenia. PMID: 20471030
This is the first report linking hyperglycemia with thyroid hormone binding protein CRYM and suggests that the role of CRYM in diabetic complications should be further investigated. PMID: 20018174
Identification of CRYM as a candidate responsible for nonsyndromic deafness, through cDNA microarray analysis of human cochlear and vestibular tissues PMID: 12471561
up-regulation of mu-crystallin may play a specific and important role in pathogenesis of facioscapulohumeral muscular dystrophy PMID: 17451686
CRYM is a novel androgen-regulated gene whose expression is elevated in prostate cancer but down-regulated in castration therapy-resistant tumors. PMID: 19353593
Using shotgun mass spectrometry, we found this protein differentially expressed in the dorsolateral prefrontal cortex from patients with schizophrenia. PMID: 19165527
This protein has been found differentially expressed in the dorsolateral prefrontal cortex from patients with schizophrenia. PMID: 19110265

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Involvement in disease

Deafness, autosomal dominant, 40 (DFNA40)

Subcellular Location

Cytoplasm.

Protein Families

Ornithine cyclodeaminase/mu-crystallin family

Tissue Specificity

Expressed in neural tissue, muscle and kidney.

Database Links

HGNC: 2418

OMIM: 123740

KEGG: hsa:1428

STRING: 9606.ENSP00000219599

UniGene: Hs.924

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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