CUL1 Antibody

Datasheet
Code CSB-PA614436ESR2HU
Size US$167Purchase it in Cusabio online store
(only available for customers from the US)
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  • Immunohistochemistry of paraffin-embedded human colon cancer using CSB-PA614436ESR2HU at dilution of 1:100

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Product Details

Full Product Name Rabbit anti-Homo sapiens (Human) CUL1 Polyclonal antibody
Uniprot No. Q13616
Target Names CUL1
Alternative Names CUL 1 antibody; CUL-1 antibody; CUL1 antibody; CUL1_HUMAN antibody; Cullin-1 antibody; Cullin1 antibody; MGC149834 antibody; MGC149835 antibody
Raised in Rabbit
Species Reactivity Human
Immunogen Recombinant Human Cullin-1 protein (500-776AA)
Immunogen Species Homo sapiens (Human)
Conjugate Non-conjugated
Clonality Polyclonal
Isotype IgG
Purification Method Antigen Affinity purified
Concentration It differs from different batches. Please contact us to confirm it.
Buffer PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Form Liquid
Tested Applications ELISA, IHC
Recommended Dilution
Application Recommended Dilution
IHC 1:20-1:200
Protocols ELISA Protocol
Immunohistochemistry (IHC) Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Target Data

Function Core component of multiple cullin-RING-based SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. SCF complexes and ARIH1 collaborate in tandem to mediate ubiquitination of target proteins
Gene References into Functions
  1. High expression of CUL1 is associated with colorectal cancer. PMID: 28560438
  2. we analyzed eight probands with clinically suspected Weaver syndrome by whole-exome sequencing and identified three mutations: a 25.4-kb deletion partially involving EZH2 and CUL1 ,a missense mutation , and a missense mutation in SUZ12 inherited from her father .In vitro functional analyses demonstrated that the identified EED and SUZ12 missense mutations cause decreased decreased trimethylation of lysine 27 of H3 PMID: 28229514
  3. DCUN1D3 has a role in activating SCFSKP2 ubiquitin E3 ligase activity through cullin-1 neddylation and cell cycle progression in tumor cells with UV damage PMID: 27542266
  4. Cullin1 and MMP-2 expressions could be novel diagnostic and prognostic markers for colorectal cancer patients. PMID: 28269751
  5. Our results demonstrate that increased CUL1 expression is significantly correlated with poor prognosis of patients with osteosarcoma. CUL1 might be an important marker and a therapeutic target for osteosarcoma. PMID: 28315506
  6. Our results demonstrate that increased CUL1 expression is significantly correlated with poor prognosis of patients with urothelial carcinoma PMID: 27312089
  7. unneddylated Cul1 is another central player involved in maintenance of the adaptor module pool through the formation of Cul1-Skp-1-F-box complexes and promotion of rapid SCF assembly PMID: 28923850
  8. Data suggest that cullin1 (Cul1) may constitute a potential therapeutic target in renal cell carcinoma (RCC). PMID: 27449035
  9. Cul1 promoted mTORC1 activity and cap-dependent translation by enhancing the ubiquitination and degradation of DEPTOR. eIF4F suppression inhibited the promoting effect of Cul1 on melanoma cell proliferation. PMID: 26717892
  10. Data show that melanoma antigen, family C, 2 protein (MAGE-C2) binds with RING-box protein 1 (Rbx1) and Cullin 1, and regulates cyclin E stability in melanoma cells. PMID: 26540345
  11. High Cul1 expression predicts worse 5-year overall and disease-specific survival rates in HCC patients. PMID: 26097587
  12. Nedd8(Q40E) cannot induce the same structural effect on Cul1-Rbx1 as wild-type Nedd8. PMID: 26632597
  13. Cullin-1 has been deeply implicated in the pathogenesis and development of prostate cancer PMID: 25973042
  14. Increased CUL1 expression in CRC cells significantly promoted cell migration and invasion abilities in vitro and peritoneal metastasis in vivo through inducing high expression of MMPs. PMID: 25652886
  15. Our data indicated that Cul1 expression significantly increased in human glioma, and it may be involved in proliferation, migration and invasion of glioma cells. PMID: 25201578
  16. In papillary thyroid carcinoma, cytoplasmic expression of Cul1 was correlated with tumor occurrence, N stage and Cyclin D1 expression. Nuclear Cul1 expression was negatively correlated with tumor occurence. PMID: 24819753
  17. We discuss how these results can explain the rapid association of Cdc34 and SCF. PMID: 25425648
  18. By integrating our WES and CN data we identified three mutated putative candidate genes targeted by 7q deletions (CUL1, EZH2 and FLNC), with FLNC positioned within the well-characterized 7q minimally deleted region. PMID: 24349473
  19. data suggested that Cullin1 might promote the progression of non-small-cell lung cancer PMID: 24767980
  20. Studies indicate that in SCFs, Rbx1 serves as the RING-containing enzyme, Cul1 is the Cullin scaffold, and Skp1 is an adaptor, which serves to link the beta-TrCP F-box substrate-specific factor to the rest of the ligase. PMID: 23624913
  21. Cul1 overexpression is significantly correlated with breast cancer progression and predicts worse survival. Cul1 regulates breast cancer cell proliferation, migration and invasion. PMID: 23592700
  22. Substrate binding promotes formation of the Skp1-Cul1-Fbxl3 (SCF(Fbxl3)) protein complex. PMID: 24085301
  23. The Epstein-Barr virus protein BPLF1, is targeted to cullin-RING ubiquitin ligases (CRLs) via the interaction of the conserved helix-2 with helix-23 of cullins, at a site involved in electrostatic interaction with CAND1. PMID: 22474075
  24. CUL1 promotes human trophoblast cell invasion and dysregulation of CUL1 expression may be associated with pre-eclampsia. PMID: 23429288
  25. Skp1-Cul1-F-box ubiquitin ligase (SCF(betaTrCP))-mediated destruction of the ubiquitin-specific protease USP37 during G2-phase promotes mitotic entry PMID: 23027877
  26. Deconjugation of Nedd8 from Cul1 is directly regulated by Skp1-F-box and substrate, and the COP9 signalosome inhibits deneddylated SCF by a noncatalytic mechanism. PMID: 22767593
  27. RBX1-containing cullin-RING ligases can bind glomulin (GLMN), which binds RING-box1's RING domain, regulating the RBX1-CUL1-containing SCF(FBW7) complex. PMID: 22748924
  28. Glomulin binds Rbx1 and regulates cullin-1 RING ligase-mediated turnover of Fbw7. PMID: 22405651
  29. binding of Cul1-Rbx1 to Cul7-Rbx1 is mediated via heterodimerization of Fbxw8 with other F-box proteins which function to recruit substrates into the E3 ligase complex PMID: 21946088
  30. Association with CUL1 haplotype indicates a possible role of CUL1 variation(s) in rheumatoid arthritis and its response to methotrexate. PMID: 21738080
  31. Our data indicated that Cullin1 may be an important marker for human gastric cancer lymph node metastasis and prognosis. PMID: 21190721
  32. Data show that CRN7 interacted with Cullin1 and Roc1 to form a novel SCF-like E3 complex. PMID: 21130766
  33. The Cul1 expression is significantly correlated with melanoma development at early stages, implying targeting Cul1 may serve as a potential therapy for melanoma. PMID: 20518860
  34. Cul1 regulates melanoma cell growth and cell cycle progression through degradation of p27 PMID: 20878082
  35. Rictor forms a complex with Cullin-1 to promote SGK1 ubiquitination and destruction. PMID: 20832730
  36. crystal structure of the Cul1-Rbx1-Skp1-F boxSkp2 SCF complex PMID: 11961546
  37. The COP9 signalosome inhibits p27(kip1) degradation and impedes G1-S phase progression via deneddylation of SCF Cul1. PMID: 11967155
  38. Data show that interference with Skp1 function through expression of the Cul1-N252 mutant results in the formation of multinucleated cells, centrosome and mitotic spindle abnormalities, and impaired chromosome segregation. PMID: 12417738
  39. p120(CAND1) selectively binds to unneddylated CUL1 and is dissociated by CUL1 neddylation PMID: 12504025
  40. unnedyylated protein binds to CAND1 and regulates the formation of SCF ubiquitin E3 ligase complex PMID: 12504026
  41. results suggest a unique role for NEDD8-specific protease 1(DEN1) in regulating the modification of cullin 1 by Nedd8 protein PMID: 12759363
  42. regulation of CUL1 expression may affect the susceptibility of rheumatoid arthritis via altering lymphocyte signal transduction PMID: 15759013
  43. CAND1 & COP9 signalosome (CSN), major deneddylase of cullins, bind to unneddylated CUL1 in mutually exclusive way. Binding of CSN to CUL1 required 4 helix bundle in CUL1 C-terminal domain, which was wrapped around by CAND1 in CAND1-CUL1-Rbx1 complex. PMID: 16036220
  44. the SCF complex (Skp1/Cul1/F-box protein/Roc1) intervenes in the surveillance of Cdh1 cellular abundance in S-phase PMID: 16123585
  45. Results describe the regulation of neddylation and deneddylation of cullin1 by Nedd8 in SCFSkp2 ubiquitin ligase by F-box protein and substrate. PMID: 16861300
  46. Thiazolidinediones modulate the expression of beta catenin and other cell cycle proteins by targeting CUL1 independently of PPARG. PMID: 17569795
  47. CUL1 ECTD (extreme C-terminal domain; spanning the C-terminal 50 amino acids), did not contribute to CUL1's stable association with ROC1. PMID: 18723677
  48. p18-Cyclin E by the Skp1-Cul1-Fbw7 (SCF) complex and its interaction with the Fbw7 protein isoforms can take place independently of phosphorylation of p18-Cyclin E at a C-terminal phosphodegron. PMID: 18784078
  49. SCCRO recruits Ubc12 approximately NEDD8 to the CAND1-Cul1-ROC1 complex but that this is not sufficient to dissociate or overcome the inhibitory effects of CAND1 on cullin neddylation PMID: 18826954
  50. CUL1 may function as a tumor suppressor by regulating PLK4 protein levels and thereby restraining excessive daughter centriole formation at maternal centrioles. PMID: 19679553
  51. Cdc34 binds SCF with nanomolar affinity, the complex is extremely dynamic; these properties are enabled by rapid association driven by electrostatic interactions between the acidic tail of Cdc34 and a basic 'canyon' in the Cul1 subunit of SCF. PMID: 19945379

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Protein Families Cullin family
Tissue Specificity Expressed in lung fibroblasts.
Database Links

HGNC: 2551

OMIM: 603134

KEGG: hsa:8454

STRING: 9606.ENSP00000326804

UniGene: Hs.146806

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