Product Details

Uniprot No.

Target Names

Alternative Names

CUL 1 antibody; CUL-1 antibody; CUL1 antibody; CUL1_HUMAN antibody; Cullin-1 antibody; Cullin1 antibody; MGC149834 antibody; MGC149835 antibody

Species Reactivity

Human, Mouse

Immunogen

A synthesized peptide derived from human CUL1

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

19B9

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
FC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

CUL1 serves as the essential scaffold protein within SCF (Skp1-Cullin-F-box) E3 ubiquitin ligase complexes, orchestrating the ubiquitination and subsequent proteasomal degradation of numerous regulatory proteins. This central role in controlling cell cycle progression, signal transduction, and transcriptional regulation makes CUL1 a critical target for researchers investigating cancer biology, cell proliferation mechanisms, and protein homeostasis pathways.

This recombinant monoclonal antibody, generated against a synthetic peptide derived from human CUL1, offers the reproducibility and consistency that demanding experimental workflows require. As a sequence-defined recombinant clone, it eliminates the lot-to-lot variability inherent in traditional hybridoma-derived antibodies, ensuring your results remain comparable across extended studies and collaborative projects.

Validation data demonstrates reliable performance across multiple experimental platforms. Western blot analysis confirms specific detection at the expected 90 kDa molecular weight in human K562 and Jurkat cell lysates, as well as mouse NIH/3T3 cells, supporting cross-species reactivity between human and mouse samples. The antibody performs effectively at dilutions ranging from 1:500 to 1:2000 for immunoblotting applications. Flow cytometry validation in HeLa cells shows clear separation between specific staining and isotype control populations, with optimal results achieved at 1:50 to 1:200 dilutions following fixation and permeabilization.

The unconjugated format provides flexibility for pairing with your preferred secondary detection systems, while the glycerol-stabilized formulation supports long-term storage stability. Whether you are investigating SCF complex assembly, characterizing ubiquitin-proteasome pathway dynamics, or exploring CUL1's emerging roles in tumorigenesis, this antibody delivers the specificity and consistency your research demands.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Core component of multiple cullin-RING-based SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. SCF complexes and ARIH1 collaborate in tandem to mediate ubiquitination of target proteins. In the SCF complex, serves as a rigid scaffold that organizes the SKP1-F-box protein and RBX1 subunits. May contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and exchange of the substrate recognition component is mediated by TIP120A/CAND1. The functional specificity of the SCF complex depends on the F-box protein as substrate recognition component. SCF(BTRC) and SCF(FBXW11) direct ubiquitination of CTNNB1 and participate in Wnt signaling. SCF(FBXW11) directs ubiquitination of phosphorylated NFKBIA. SCF(BTRC) directs ubiquitination of NFKBIB, NFKBIE, ATF4, SMAD3, SMAD4, CDC25A, FBXO5 and probably NFKB2. SCF(BTRC) and/or SCF(FBXW11) direct ubiquitination of CEP68. SCF(SKP2) directs ubiquitination of phosphorylated CDKN1B/p27kip and is involved in regulation of G1/S transition. SCF(SKP2) directs ubiquitination of ORC1, CDT1, RBL2, ELF4, CDKN1A, RAG2, FOXO1A, and probably MYC and TAL1. SCF(FBXW7) directs ubiquitination of CCNE1, NOTCH1 released notch intracellular domain (NICD), and probably PSEN1. SCF(FBXW2) directs ubiquitination of GCM1. SCF(FBXO32) directs ubiquitination of MYOD1. SCF(FBXO7) directs ubiquitination of BIRC2 and DLGAP5. SCF(FBXO33) directs ubiquitination of YBX1. SCF(FBXO1) directs ubiquitination of BCL6 and DTL but does not seem to direct ubiquitination of TP53. SCF(BTRC) mediates the ubiquitination of NFKBIA at 'Lys-21' and 'Lys-22'; the degradation frees the associated NFKB1-RELA dimer to translocate into the nucleus and to activate transcription. SCF(CCNF) directs ubiquitination of CCP110. SCF(FBXL3) and SCF(FBXL21) direct ubiquitination of CRY1 and CRY2. SCF(FBXO9) directs ubiquitination of TTI1 and TELO2. SCF(FBXO10) directs ubiquitination of BCL2.

Gene References into Functions

High expression of CUL1 is associated with colorectal cancer. PMID: 28560438
we analyzed eight probands with clinically suspected Weaver syndrome by whole-exome sequencing and identified three mutations: a 25.4-kb deletion partially involving EZH2 and CUL1 ,a missense mutation , and a missense mutation in SUZ12 inherited from her father .In vitro functional analyses demonstrated that the identified EED and SUZ12 missense mutations cause decreased decreased trimethylation of lysine 27 of H3 PMID: 28229514
DCUN1D3 has a role in activating SCFSKP2 ubiquitin E3 ligase activity through cullin-1 neddylation and cell cycle progression in tumor cells with UV damage PMID: 27542266
Cullin1 and MMP-2 expressions could be novel diagnostic and prognostic markers for colorectal cancer patients. PMID: 28269751
Our results demonstrate that increased CUL1 expression is significantly correlated with poor prognosis of patients with osteosarcoma. CUL1 might be an important marker and a therapeutic target for osteosarcoma. PMID: 28315506
Our results demonstrate that increased CUL1 expression is significantly correlated with poor prognosis of patients with urothelial carcinoma PMID: 27312089
unneddylated Cul1 is another central player involved in maintenance of the adaptor module pool through the formation of Cul1-Skp-1-F-box complexes and promotion of rapid SCF assembly PMID: 28923850
Data suggest that cullin1 (Cul1) may constitute a potential therapeutic target in renal cell carcinoma (RCC). PMID: 27449035
Cul1 promoted mTORC1 activity and cap-dependent translation by enhancing the ubiquitination and degradation of DEPTOR. eIF4F suppression inhibited the promoting effect of Cul1 on melanoma cell proliferation. PMID: 26717892
Data show that melanoma antigen, family C, 2 protein (MAGE-C2) binds with RING-box protein 1 (Rbx1) and Cullin 1, and regulates cyclin E stability in melanoma cells. PMID: 26540345
High Cul1 expression predicts worse 5-year overall and disease-specific survival rates in HCC patients. PMID: 26097587
Nedd8(Q40E) cannot induce the same structural effect on Cul1-Rbx1 as wild-type Nedd8. PMID: 26632597
Cullin-1 has been deeply implicated in the pathogenesis and development of prostate cancer PMID: 25973042
Increased CUL1 expression in CRC cells significantly promoted cell migration and invasion abilities in vitro and peritoneal metastasis in vivo through inducing high expression of MMPs. PMID: 25652886
Our data indicated that Cul1 expression significantly increased in human glioma, and it may be involved in proliferation, migration and invasion of glioma cells. PMID: 25201578
In papillary thyroid carcinoma, cytoplasmic expression of Cul1 was correlated with tumor occurrence, N stage and Cyclin D1 expression. Nuclear Cul1 expression was negatively correlated with tumor occurence. PMID: 24819753
We discuss how these results can explain the rapid association of Cdc34 and SCF. PMID: 25425648
By integrating our WES and CN data we identified three mutated putative candidate genes targeted by 7q deletions (CUL1, EZH2 and FLNC), with FLNC positioned within the well-characterized 7q minimally deleted region. PMID: 24349473
data suggested that Cullin1 might promote the progression of non-small-cell lung cancer PMID: 24767980
Studies indicate that in SCFs, Rbx1 serves as the RING-containing enzyme, Cul1 is the Cullin scaffold, and Skp1 is an adaptor, which serves to link the beta-TrCP F-box substrate-specific factor to the rest of the ligase. PMID: 23624913
Cul1 overexpression is significantly correlated with breast cancer progression and predicts worse survival. Cul1 regulates breast cancer cell proliferation, migration and invasion. PMID: 23592700
Substrate binding promotes formation of the Skp1-Cul1-Fbxl3 (SCF(Fbxl3)) protein complex. PMID: 24085301
The Epstein-Barr virus protein BPLF1, is targeted to cullin-RING ubiquitin ligases (CRLs) via the interaction of the conserved helix-2 with helix-23 of cullins, at a site involved in electrostatic interaction with CAND1. PMID: 22474075
CUL1 promotes human trophoblast cell invasion and dysregulation of CUL1 expression may be associated with pre-eclampsia. PMID: 23429288
Skp1-Cul1-F-box ubiquitin ligase (SCF(betaTrCP))-mediated destruction of the ubiquitin-specific protease USP37 during G2-phase promotes mitotic entry PMID: 23027877
Deconjugation of Nedd8 from Cul1 is directly regulated by Skp1-F-box and substrate, and the COP9 signalosome inhibits deneddylated SCF by a noncatalytic mechanism. PMID: 22767593
RBX1-containing cullin-RING ligases can bind glomulin (GLMN), which binds RING-box1's RING domain, regulating the RBX1-CUL1-containing SCF(FBW7) complex. PMID: 22748924
Glomulin binds Rbx1 and regulates cullin-1 RING ligase-mediated turnover of Fbw7. PMID: 22405651
binding of Cul1-Rbx1 to Cul7-Rbx1 is mediated via heterodimerization of Fbxw8 with other F-box proteins which function to recruit substrates into the E3 ligase complex PMID: 21946088
Association with CUL1 haplotype indicates a possible role of CUL1 variation(s) in rheumatoid arthritis and its response to methotrexate. PMID: 21738080
Our data indicated that Cullin1 may be an important marker for human gastric cancer lymph node metastasis and prognosis. PMID: 21190721
Data show that CRN7 interacted with Cullin1 and Roc1 to form a novel SCF-like E3 complex. PMID: 21130766
The Cul1 expression is significantly correlated with melanoma development at early stages, implying targeting Cul1 may serve as a potential therapy for melanoma. PMID: 20518860
Cul1 regulates melanoma cell growth and cell cycle progression through degradation of p27 PMID: 20878082
Rictor forms a complex with Cullin-1 to promote SGK1 ubiquitination and destruction. PMID: 20832730
crystal structure of the Cul1-Rbx1-Skp1-F boxSkp2 SCF complex PMID: 11961546
The COP9 signalosome inhibits p27(kip1) degradation and impedes G1-S phase progression via deneddylation of SCF Cul1. PMID: 11967155
Data show that interference with Skp1 function through expression of the Cul1-N252 mutant results in the formation of multinucleated cells, centrosome and mitotic spindle abnormalities, and impaired chromosome segregation. PMID: 12417738
p120(CAND1) selectively binds to unneddylated CUL1 and is dissociated by CUL1 neddylation PMID: 12504025
unnedyylated protein binds to CAND1 and regulates the formation of SCF ubiquitin E3 ligase complex PMID: 12504026
results suggest a unique role for NEDD8-specific protease 1(DEN1) in regulating the modification of cullin 1 by Nedd8 protein PMID: 12759363
regulation of CUL1 expression may affect the susceptibility of rheumatoid arthritis via altering lymphocyte signal transduction PMID: 15759013
CAND1 & COP9 signalosome (CSN), major deneddylase of cullins, bind to unneddylated CUL1 in mutually exclusive way. Binding of CSN to CUL1 required 4 helix bundle in CUL1 C-terminal domain, which was wrapped around by CAND1 in CAND1-CUL1-Rbx1 complex. PMID: 16036220
the SCF complex (Skp1/Cul1/F-box protein/Roc1) intervenes in the surveillance of Cdh1 cellular abundance in S-phase PMID: 16123585
Results describe the regulation of neddylation and deneddylation of cullin1 by Nedd8 in SCFSkp2 ubiquitin ligase by F-box protein and substrate. PMID: 16861300
Thiazolidinediones modulate the expression of beta catenin and other cell cycle proteins by targeting CUL1 independently of PPARG. PMID: 17569795
CUL1 ECTD (extreme C-terminal domain; spanning the C-terminal 50 amino acids), did not contribute to CUL1's stable association with ROC1. PMID: 18723677
p18-Cyclin E by the Skp1-Cul1-Fbw7 (SCF) complex and its interaction with the Fbw7 protein isoforms can take place independently of phosphorylation of p18-Cyclin E at a C-terminal phosphodegron. PMID: 18784078
SCCRO recruits Ubc12 approximately NEDD8 to the CAND1-Cul1-ROC1 complex but that this is not sufficient to dissociate or overcome the inhibitory effects of CAND1 on cullin neddylation PMID: 18826954
CUL1 may function as a tumor suppressor by regulating PLK4 protein levels and thereby restraining excessive daughter centriole formation at maternal centrioles. PMID: 19679553

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Protein Families

Cullin family

Tissue Specificity

Expressed in lung fibroblasts.

Database Links

HGNC: 2551

OMIM: 603134

KEGG: hsa:8454

STRING: 9606.ENSP00000326804

UniGene: Hs.146806

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Code	CSB-RA444398A0HU
Size	US$210
	Order now
Image	Western Blot Positive WB detected in: K562 whole cell lysate(30µg),Jurkat whole cell lysate(30µg), NIH/3T3 whole cell lysate(30µg) All lanes: Cullin 1 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 90 kDa Observed band size: 90 kDa Exposure time：2min Overlay Peak curve showing Hela cells stained with CSB-RA444398A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
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CUL1 Recombinant Monoclonal Antibody

Product Details

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Target Background