CUTC Antibody

Datasheet

Code	CSB-PA873651LA01HU
Size	US$166
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Image	IHC image of CSB-PA873651LA01HU diluted at 1:400 and staining in paraffin-embedded human gastric cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. IHC image of CSB-PA873651LA01HU diluted at 1:400 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunofluorescence staining of Hela cells with CSB-PA873651LA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) CUTC Polyclonal antibody

Uniprot No.

Q9NTM9

Target Names

CUTC

Alternative Names

CGI 32 antibody; CGI32 antibody; Copper homeostasis protein cutC homolog antibody; cutC antibody; CutC copper transporter homolog (E. coli) antibody; CUTC_HUMAN antibody; RP11-483F11.3 antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Copper homeostasis protein cutC homolog protein (1-64AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The CUTC Antibody (Product code: CSB-PA873651LA01HU) is Non-conjugated. For CUTC Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA873651LB01HU	CUTC Antibody, HRP conjugated	ELISA
FITC	CSB-PA873651LC01HU	CUTC Antibody, FITC conjugated
Biotin	CSB-PA873651LD01HU	CUTC Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, IHC, IF

Recommended Dilution

Application	Recommended Dilution
IHC	1:200-1:500
IF	1:50-1:200

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

May play a role in copper homeostasis. Can bind one Cu(1+) per subunit.

Gene References into Functions

present study reveals copper induced damage in cells upon hCutC silencing and provides evidence for the role of hCutC protein in intracellular copper homeostasis PMID: 26660891
Results suggest that hCutC functions as an enzyme with Cu(I) as a cofactor rather than a copper transporter and the potential Cu(I)-binding site consists of the two Cys residues and other conserved residues in the vicinity. PMID: 19878721
Cloning, expression and subcellular localization of CutC. PMID: 16182249

Subcellular Location

Cytoplasm. Nucleus. Note=The overexpressed protein is detected in the cytoplasm, and depending on the cell line, also in the nucleus.

Protein Families

CutC family

Tissue Specificity

Ubiquitous.

Database Links

HGNC: 24271

OMIM: 610101

KEGG: hsa:51076

STRING: 9606.ENSP00000359507

UniGene: Hs.16606

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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