CYP51A1 Antibody

Datasheet

Code	CSB-PA614993LA01HU
Size	US$166
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Image	Western Blot Positive WB detected in: MCF-7 whole cell lysate All lanes: CYP51A1 antibody at 2.7µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 57, 47 kDa Observed band size: 57 kDa Immunohistochemistry of paraffin-embedded human testis tissue using CSB-PA614993LA01HU at dilution of 1:100
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Product Details
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) CYP51A1 Polyclonal antibody

Uniprot No.

Q16850

Target Names

CYP51A1

Alternative Names

CYP51A1; CYP51; Lanosterol 14-alpha demethylase; LDM; CYPLI; Cytochrome P450 51A1; Cytochrome P450-14DM; Cytochrome P45014DM; Cytochrome P450LI; Sterol 14-alpha demethylase

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Lanosterol 14-alpha demethylase protein (270-437AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The CYP51A1 Antibody (Product code: CSB-PA614993LA01HU) is Non-conjugated. For CYP51A1 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA614993LB01HU	CYP51A1 Antibody, HRP conjugated	ELISA
FITC	CSB-PA614993LC01HU	CYP51A1 Antibody, FITC conjugated
Biotin	CSB-PA614993LD01HU	CYP51A1 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:20-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Customer Reviews and Q&A

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Target Background

Function

A cytochrome P450 monooxygenase involved in sterol biosynthesis. Catalyzes 14-alpha demethylation of lanosterol and 24,25-dihydrolanosterol likely through sequential oxidative conversion of 14-alpha methyl group to hydroxymethyl, then to carboxylaldehyde, followed by the formation of the delta 14,15 double bond in the sterol core and concomitant release of formic acid. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH-ferrihemoprotein reductase).

Gene References into Functions

the regulation of expression of human CYP51A1, the lanosterol 14alpha-demethylase, is reported. PMID: 28830911
Molecular insights regarding substrate profile, faster catalysis, and weaker susceptibility of human CYP51 to inhibition. PMID: 27313059
Suggest that GDF9, possibly with FSH, may play significant roles in the regulation of cholesterol biosynthesis and the expression of CYP51A1 in granulosa cells which might be a predictor for unfertilization. PMID: 24711211
Data show that forkhead transcription factor 4 (FoxO4) interacts with sterol regulatory element binding protein (SREBP)2 and hypoxia inducible factor (HIF)2alpha to modulate lanosterol 14alpha demethylase (CYP51) promoter activity. PMID: 24353279
Our results indicate a new link between a cholesterol synthesis gene CYP51A1 and pregnancy pathologies. PMID: 24358204
The studied azoles selectively interacted with human cytochrome P450 51A1, which showed the highest affinity towards ketoconazole. PMID: 24502137
Low nucleotide variability of CYP51A1 is seen in cholesterol and bile acid synthesis and xenobiotic metabolism pathways. PMID: 24362992
The new CYP51A1 inhibitor 2-((3,4-dichlorophenethyl)(propyl)amino)-1-(pyridin-3-yl)ethanol (LEK-935) on the proteome of primary human hepatocytes were analyzed. PMID: 22180046
Studies indicate that CPY51 structures from various eukaryotic organisms are strikingly similar. PMID: 20547249
The substantial conformational changes in the B' helix and F-G loop regions are induced upon ligand binding, consistent with the membrane nature of the protein and its substrate. PMID: 20149798
A cAMP-responsive element binding site is essential for sterol regulation of the human lanosterol 14alpha-demethylase gene. PMID: 12145339
Plays primary roles in determining strength of interactions with azoles PMID: 15611056
the liver X receptor alpha directly silencing the expression of two key cholesterologenic enzymes (lanosterol 14alpha-demethylase (CYP51A1), and squalene synthase (farnesyl diphosphate farnesyl transferase 1)) via novel negative LXR DNA response elements PMID: 18676367

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Subcellular Location

Endoplasmic reticulum membrane; Single-pass membrane protein. Microsome membrane; Single-pass membrane protein.

Protein Families

Cytochrome P450 family

Tissue Specificity

Ubiquitously expressed with highest levels in testis, ovary, adrenal, prostate, liver, kidney and lung.

Database Links

HGNC: 2649

OMIM: 601637

KEGG: hsa:1595

STRING: 9606.ENSP00000003100

UniGene: Hs.417077

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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