CYP8B1 Antibody

Datasheet

Code	CSB-PA006462LA01HU
Size	US$166
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Image	Western Blot Positive WB detected in: Rat kidney tissue All lanes: CYP8B1 antibody at 6.4μg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 59 kDa Observed band size: 59 kDa IHC image of CSB-PA006462LA01HU diluted at 1:300 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. IHC image of CSB-PA006462LA01HU diluted at 1:300 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunofluorescence staining of HepG2 cells with CSB-PA006462LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) CYP8B1 Polyclonal antibody

Uniprot No.

Q9UNU6

Target Names

CYP8B1

Alternative Names

7-alpha-hydroxy-4-cholesten-3-one 12-alpha-hydroxylase antibody; 7-alpha-hydroxycholest-4-en-3-one 12-alpha-hydroxylase antibody; CP8B antibody; CP8B1_HUMAN antibody; CYP12 antibody; CYP8B1 antibody; CYPVIIIB1 antibody; Cytochrome P450 8B1 antibody; Cytochrome P450 subfamily VIIIB (sterol 12 alpha hydroxylase) polypeptide 1 antibody; Cytochrome P450; family 8; subfamily B; polypeptide 1 antibody; Sterol 12-alpha-hydroxylase antibody

Raised in

Rabbit

Species Reactivity

Human, Rat

Immunogen

Recombinant Human 7-alpha-hydroxycholest-4-en-3-one 12-alpha-hydroxylase protein (303-440AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The CYP8B1 Antibody (Product code: CSB-PA006462LA01HU) is Non-conjugated. For CYP8B1 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA006462LB01HU	CYP8B1 Antibody, HRP conjugated	ELISA
FITC	CSB-PA006462LC01HU	CYP8B1 Antibody, FITC conjugated
Biotin	CSB-PA006462LD01HU	CYP8B1 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:200-1:500
IF	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

A cytochrome P450 monooxygenase involved in primary bile acid biosynthesis. Catalyzes the 12alpha-hydroxylation of 7alpha-hydroxy-4-cholesten-3-one, an intermediate metabolite in cholic acid biosynthesis. Controls biliary balance of cholic acid and chenodeoxycholic acid, ultimately regulating the intestinal absorption of dietary lipids. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH--hemoprotein reductase).

Gene References into Functions

RORalpha is a key regulator of diurnal rhythm and fasting induction of CYP8B1, which regulates bile acid composition and serum and liver cholesterol levels. PMID: 24226095
The single nucleotide polymorphism rs3732860 in the 3'-untranslated region of the CYP8B1 gene is associated with risk of Gallstone Disease in Chinese Han. PMID: 23216301
SNP rs3732860 of CYP8B1 gene is associated with gallstone disease in this Chinese population. PMID: 22093981
Cholesterol loading reduces SREBP-1 mRNA expression in addition to reducing functional SREBP-1 protein, and results in decreasing CYP8B1 gene transcription. PMID: 15249218
Studies indicate a homogenous pattern for CYP8B1 expression in human liver, which was even rather than zonal. PMID: 15891895
Isoflavone-related induction of 12-alpha-hydroxylase (CYP8B1) was studied in vitro and murine in vivo models. PMID: 17585019

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Subcellular Location

Endoplasmic reticulum membrane; Single-pass membrane protein. Microsome membrane; Single-pass membrane protein.

Protein Families

Cytochrome P450 family

Tissue Specificity

Liver.

Database Links

HGNC: 2653

OMIM: 602172

KEGG: hsa:1582

STRING: 9606.ENSP00000318867

UniGene: Hs.447793

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Protein Service

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Related pathways

PPAR signaling pathway

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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