Ctcfl Antibody

Datasheet

Code	CSB-PA006139LA01MO
Size	US$166
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Image	Western blot All lanes: Ctcfl antibody at 6µg/ml + Hela whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 74, 75 kDa Observed band size: 74 kDa
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Target Background

Product Details

Full Product Name

Rabbit anti-Mus musculus (Mouse) Ctcfl Polyclonal antibody

Uniprot No.

A2APF3

Target Names

Ctcfl

Alternative Names

Ctcfl antibody; Boris antibody; Transcriptional repressor CTCFL antibody; Brother of the regulator of imprinted sites antibody; CCCTC-binding factor antibody; CTCF paralog antibody; CTCF-like protein antibody

Raised in

Rabbit

Species Reactivity

Mouse, Human

Immunogen

Recombinant Mouse Transcriptional repressor CTCFL protein (1-636AA)

Immunogen Species

Mus musculus (Mouse)

Conjugate

Non-conjugated

The Ctcfl Antibody (Product code: CSB-PA006139LA01MO) is Non-conjugated. For Ctcfl Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA006139LB01MO	Ctcfl Antibody, HRP conjugated	ELISA
FITC	CSB-PA006139LC01MO	Ctcfl Antibody, FITC conjugated
Biotin	CSB-PA006139LD01MO	Ctcfl Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid

Tested Applications

ELISA, WB

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000

Protocols

ELISA Protocol
Western Blotting(WB) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Background

Function

Testis-specific DNA binding protein responsible for insulator function, nuclear architecture and transcriptional control, which probably acts by recruiting epigenetic chromatin modifiers. Plays a key role in gene imprinting in male germline, by participating in the establishment of differential methylation at the IGF2/H19 imprinted control region (ICR). Directly binds the unmethylated H19 ICR and recruits the PRMT7 methyltransferase, leading to methylate histone H4 'Arg-3' to form H4R3sme2. This probably leads to recruit de novo DNA methyltransferases at these sites. Seems to act as tumor suppressor. In association with DNMT1 and DNMT3B, involved in activation of BAG1 gene expression by binding to its promoter. Required for dimethylation of H3 lysine 4 (H3K4me2) of MYC and BRCA1 promoters.

Gene References into Functions

CTCF and BORIS cooperate with additional Testis-specific transcriptional regulators to regulate gene expression in developing male gametes. PMID: 28145452
The TGFB pathway as most affected by embryonic Ctcfl expression. PMID: 26169830
down-regulation of endogenous BORIS by specific shRNAs inhibited both RNA transcription and cell cycle progression. The results altogether suggest a role for BORIS in coordinating S phase events with mitosis. PMID: 22724006
Rb2 coimmunolocalizes with the chromatin insulator CCCTC-binding factor (CTCF) and BORIS in T-antigen-positive but not in T-antigen-negative cells. PMID: 22544282
These findings show that BORIS expression is more widespread than previously believed, and suggest a role for BORIS in nucleolar function. PMID: 21811597
These findings define transcriptional regulation of CST (Gal3st1) expression as a critical role for BORIS in spermatogenesis. PMID: 20231363
CTCFL/BORIS is a methylation-independent DNA-binding protein that preferentially binds to the paternal H19 differentially methylated region PMID: 18632606
DNA damage-induced and ATR/Rfx1-mediated increase of miR-709 expression in exposed testes may be a protective mechanism that effectively decreases a cellular level of BORIS to prevent massive aberrant erasure of DNA methylation after radiation exposure. PMID: 19029807

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Subcellular Location

Cytoplasm. Nucleus.

Protein Families

CTCF zinc-finger protein family

Tissue Specificity

Testis-specific.

Database Links

KEGG: mmu:664799

STRING: 10090.ENSMUSP00000091845

UniGene: Mm.389396

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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