DAG1 Antibody

Code CSB-PA613486NA01HU
Size US$299
  • IHC image of CSB-PA613486NA01HU diluted at 1:300 and staining in paraffin-embedded human glioma performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Product Details

Full Product Name Rabbit anti-Homo sapiens (Human) DAG1 Polyclonal antibody
Uniprot No. Q14118
Target Names DAG1
Alternative Names 156DAG antibody; A3a antibody; AGRNR antibody; Alpha dystroglycan antibody; Alpha-DG antibody; Beta-DG antibody; Beta-dystroglycan antibody; DAG antibody; Dag1 antibody; DAG1_HUMAN antibody; Dystroglycan 1 (dystrophin associated glycoprotein 1) antibody; Dystroglycan antibody; Dystrophin associated glycoprotein 1 antibody; Dystrophin-associated glycoprotein 1 antibody; OTTHUMP00000210857 antibody; OTTHUMP00000210858 antibody
Raised in Rabbit
Species Reactivity Human
Immunogen Peptide sequence from Human Dystroglycan protein (726-744AA)
Immunogen Species Homo sapiens (Human)
Conjugate Non-conjugated
Clonality Polyclonal
Isotype IgG
Purification Method >95%, Protein G purified
Concentration It differs from different batches. Please contact us to confirm it.
Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form Liquid
Tested Applications ELISA, IHC
Recommended Dilution
Application Recommended Dilution
IHC 1:200-1:500
Protocols ELISA Protocol
Immunohistochemistry (IHC) Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Background

(From Uniprot)
The dystroglycan complex is involved in a number of processes including laminin and basement membrane assembly, sarcolemmal stability, cell survival, peripheral nerve myelination, nodal structure, cell migration, and epithelial polarization.; FUNCTION
Gene References into Functions
  1. Study identified beta-dystroglycan as a substrate of WWP1 and found that the muscular dystrophy-causing mutation of WWP1 renders the enzyme hyperactive by relieving autoinhibition. PMID: 29635000
  2. ISPD and FKTN are essential for the incorporation of ribitol into alpha-dystroglycan. PMID: 27194101
  3. N-terminal alpha Dystroglycan ELISA signals were significantly reduced in Duchenne muscular dystrophy serum relative to serum from otherwise normal controls. PMID: 27854211
  4. TMEM5 is a UDP-xylosyl transferase that elaborates the O-mannose glycan structure on alpha-dystroglycan. The authors demonstrate in a zebrafish model as well as in a human patient that defects in TMEM5 result in muscular dystrophy in combination with abnormal brain development. PMID: 27130732
  5. Our results strongly suggest that the balance and integrity between the dystroglycan alpha and beta subunits are indispensable and responsible for the cell differentiation and proliferation in acute leukemia cells. PMID: 28591567
  6. interation of DG with laminin and dynamin is involved in the regulation of AQP4 internalization PMID: 27788222
  7. The Muscular Dystrophy Gene TMEM5 Encodes a Ribitol beta1,4-Xylosyltransferase Required for the Functional Glycosylation of Dystroglycan. PMID: 27733679
  8. Data show that CD93 antigen proved to be phosphorylated on tyrosine 628 and 644 following cell adhesion on laminin through dystroglycan. PMID: 26848865
  9. alpha-DG-N removal as an important posttranslational control of endometrial receptivity and uterine fluid alpha-DG-N as a potential biomarker for receptivity in women. PMID: 26077903
  10. Phosphorylation within the cysteine-rich region of dystrophin enhances its association with beta-dystroglycan and identifies a potential novel therapeutic target for skeletal muscle wasting. PMID: 25082828
  11. Novel mutations in DAG1 are associated with asymptomatic hyperCKemia with hypoglycosylation of alpha-dystroglycan. PMID: 25503980
  12. Reduction of alpha-dystroglycan expression is correlated with glioma. PMID: 25139094
  13. Depletion of DAG resulted in altered morphology and reduced properties of differentiated HL-60 cells, including chemotaxis, respiratory burst, phagocytic activities and markers of differentiation, implicating DAG as a protein involved in differentiation. PMID: 24792180
  14. A report of a homozygous novel DAG1 missense mutation c.2006G>T in the beta-subunit of dystroglycan in two Libyan siblings with with a novel muscle-eye-brain disease-like phenotype with multicystic leucodystrophy. PMID: 24052401
  15. The study provides evidence for at least three separate pools of dystroglycan complexes within myofibers that differ in composition and are differentially affected by loss of dystrophin. PMID: 23951345
  16. These data suggest that proteolysis, tyrosine phosphorylation and translocation of dystroglycan to the nucleus resulting in altered gene transcription could be important mechanisms in the progression of prostate cancer. PMID: 24077328
  17. T192M point-mutation in dystroglycan leads to a weaker interactions with laminin-1, which leads to hypoglycosylation and which finally leads to the limb girdle disease. PMID: 24361964
  18. GTDC2 generates CTD110.6 antibody-reactive N-acetylglucosamine epitopes on the O-mannosylated alpha-dystroglycan. PMID: 24041696
  19. the Dystroglycan-mediated cortical microtubule anchoring, the disruption of which initiates gastrulation EMT. PMID: 23940118
  20. Loss of alpha-dystroglycan and increased CD133 expression are frequent events in human colon cancer and evaluation of CD133 expression could help to identify high-risk colon cancer patients. PMID: 22964035
  21. Loss of LARGE2 disrupts functional glycosylation of alpha-dystroglycan in prostate cancer PMID: 23223448
  22. The loss of dystroglycan during tumorigenesis leads to an increased ability for tumor growth. PMID: 22996647
  23. novel role for HNK-1ST as a tumor suppressor controlling the functional glycans on alpha-DG and the importance of sulfate transfer in the glycosylation of alpha-DG. PMID: 22801424
  24. Dynamics of expression patterns of dystroglycan in human glioblastoma PMID: 22307776
  25. The virus-induced perturbation of alpha6beta1 integrin signalling critically depended on high-affinity Lassa virus binding to dystroglycan and dystroglycan's cytoplasmic domain. PMID: 22405130
  26. Data suggest that throughout pregnancy, changes occur in expression and distribution of DAG1 and dystroglycan subunits in placental tissues undergoing placentation. PMID: 22138543
  27. a mechanism by which Large competes with galactosyltransferase to target GlcNAc terminals to induce the functional glycans on alpha-DG PMID: 21347376
  28. Reduced expression and altered localization of dystroglycan is common in pancreatic cancer, potentially contributing to the aggressive behavior of this disease. PMID: 20338590
  29. the ligand-binding activity of alpha-dystroglycan is conferred primarily by LARGE modification at Thr-317 and -319, within the highly conserved first 18 amino acids of the mucin-like domain PMID: 21987822
  30. These observations suggest that secreted alpha-DG-N may be transported via CSF and have yet unidentified effects on the nervous system. PMID: 21741360
  31. LARGE has a role in inducing alpha-dystroglycan hyperglycosylation in skeletal and cardiac muscle PMID: 21203384
  32. Ameloblastoma cells proliferate and are differentiated by capturing perlecan differentially with alpha-dystroglycan and integrin beta1, respectively PMID: 21255062
  33. Data show that loss of nuclear p27(kip1) is a frequent event in human RCCs and is a powerful predictor of poor outcome which, in combination with low DG expression, could help to identify high-risk patients with clear cell RCC. PMID: 20626751
  34. Beta-Dystroglycan interaction with caveolin-1 in smooth muscle is required for receptor-mediated Ca2+ release. PMID: 20736308
  35. Twenty-five glycopeptides were characterized from human alpha-dystroglycan, which provide insight to the complex in vivo O-glycosylation of alpha-dystroglycan. PMID: 20507882
  36. Biological role of dystroglycan in Schwann cell function, especially myelination, and its implications in diseases is reviewed. PMID: 20625412
  37. Beta-dystroglycan follows a conventional Importin alpha/beta-dependent nuclear import pathway PMID: 20512930
  38. Loss of alpha-dystroglycan expression is a frequent event in human oral squamous cell carcinoma PMID: 20350218
  39. analysis of the main cleavage site involved in enzymatic processing of beta-dystroglycan recombinant ectodomain by MMP-9 PMID: 19946898
  40. study identified phosphorylated O-mannosyl glycan on mucin-like domain of alpha-DG, which was required for laminin binding; patients with muscular dystrophy have defects in a postphosphoryl modification of this phosphorylated O-linked mannose PMID: 20044576
  41. Post-translational disruption of dystroglycan-ligand interactions in congenital muscular dystrophies. PMID: 12140558
  42. Glomeruli possess large amounts of a specifically composed complex; this complex may undergo changes in human glomerular disease; and flattening of foot processes is directly associated with dissociation of laminin-dystroglycan complexes. PMID: 12386278
  43. hAG-2 and hAG-3, human homologues of genes involved in differentiation, are associated with oestrogen receptor-positive breast tumours and interact with metastasis gene C4.4a and dystroglycan. PMID: 12592373
  44. Expression is frequently reduced in human breast and colon cancers and is associated with tumor progression. PMID: 12598319
  45. in human coxsackievirus B myocarditis a focal disruption of the DAG can principally occur and may contribute to the pathogenesis of human enterovirus-induced dilated cardiomyopathy PMID: 12920582
  46. mutation of certain residues prevents both ezrin binding and the induction of actin-rich surface protrusions PMID: 15175275
  47. Cells that are defective in components of the O-mannosylation pathway showed strikingly reduced lymphocytic choriomeningitis virus infectbility. PMID: 16254364
  48. alpha-DG glycosylation may differ between neurons and glial cells in congenital musculaar dystrophy brains. PMID: 16466646
  49. DG may be involved in the progression of primary brain tumors PMID: 16575202
  50. Fukutin seems to bind to both the hypoglycosylated and fully glycosylated form of alpha-dystroglycan, and seems bind to the core area rather than the sugar chain of alpha-dystroglycan PMID: 17005282

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Involvement in disease Muscular dystrophy-dystroglycanopathy limb-girdle C9 (MDDGC9); Muscular dystrophy-dystroglycanopathy congenital with brain and eye anomalies A9 (MDDGA9)
Subcellular Location Alpha-dystroglycan: Secreted, extracellular space, SUBCELLULAR LOCATION: Beta-dystroglycan: Cell membrane, Single-pass type I membrane protein, Cytoplasm, cytoskeleton, Nucleus, nucleoplasm, Cell membrane, sarcolemma, Cell junction, synapse, postsynaptic cell membrane
Tissue Specificity Expressed in a variety of fetal and adult tissues. In epidermal tissue, located to the basement membrane. Also expressed in keratinocytes and fibroblasts.
Database Links

HGNC: 2666

OMIM: 128239

KEGG: hsa:1605

STRING: 9606.ENSP00000312435

UniGene: Hs.76111


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