DEF6 Antibody

Code CSB-PA884467LA01HU
Size US$166
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  • Western Blot
    Positive WB detected in: Mouse liver tissue, Mouse kidney tissue
    All lanes: DEF6 antibody at 4μg/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 74 kDa
    Observed band size: 74 kDa

  • Immunofluorescent analysis of U251 cells using CSB-PA884467LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

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Product Details

Full Product Name
Rabbit anti-Homo sapiens (Human) DEF6 Polyclonal antibody
Uniprot No.
Target Names
DEF6
Alternative Names
DEF-6 antibody; Def6 antibody; DEFI6_HUMAN antibody; Differentially expressed in FDCP 6 homolog antibody; Differentially expressed in FDCP 6 homologue mouse antibody; IBP antibody; IRF4-binding protein antibody; Swap70 like adapter of T cells antibody
Raised in
Rabbit
Species Reactivity
Human, Mouse
Immunogen
Recombinant Human Differentially expressed in FDCP 6 homolog protein (20-114AA)
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated

The DEF6 Antibody (Product code: CSB-PA884467LA01HU) is Non-conjugated. For DEF6 Antibody with conjugates, please check the following table.

Available Conjugates
Conjugate Product Code Product Name Application
HRP CSB-PA884467LB01HU DEF6 Antibody, HRP conjugated ELISA
FITC CSB-PA884467LC01HU DEF6 Antibody, FITC conjugated
Biotin CSB-PA884467LD01HU DEF6 Antibody, Biotin conjugated ELISA
Clonality
Polyclonal
Isotype
IgG
Purification Method
>95%, Protein G purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Form
Liquid
Tested Applications
ELISA, WB, IF
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:5000
IF 1:50-1:200
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Background

Function
Phosphatidylinositol 3,4,5-trisphosphate-dependent guanine nucleotide exchange factor (GEF) which plays a role in the activation of Rho GTPases RAC1, RhoA and CDC42. Can regulate cell morphology in cooperation with activated RAC1. Plays a role in Th2 (T helper cells) development and/or activation, perhaps by interfering with ZAP70 signaling.
Gene References into Functions
  1. this study identifies Def6 as a novel inhibitor of osteoclastogenesis in physiological and inflammatory conditions PMID: 28314855
  2. DEF6 expression may serve as an independent prognostic factor, and interacted positively with p16 toward high tumor stage and shorter survival. PMID: 27488395
  3. IBP was expressed ectopically in someoral squamous cell carcinoma samples and correlated significantly with tumor size, differentiation, clinical stage and distant metastases PMID: 23088770
  4. DEF6, a novel substrate for the Tec kinase ITK, contains a glutamine-rich aggregation-prone region and forms cytoplasmic granules that co-localize with P-bodies. PMID: 22829599
  5. Molecular cloning of IBP, a guanine nucleotide exchange factor, which is highly expressed in the immune system. PMID: 12651066
  6. IBP is a novel guanine nucleotide exchange factor and is recruited to the immunological synapse by T cell receptor engagement PMID: 12923183
  7. DEF6 not only activates Rho-family GTPases but also cooperates with activated Rac1 to exert its cellular function PMID: 17121847
  8. level of expression correlates with the degree of malignancy in breast carcinoma tissues PMID: 19393345
  9. Overexpression of the Interferon regulatory factor 4-binding protein is associated with Colorectal Carcinoma. PMID: 19679060
  10. DEF6, a novel PH-DH-like domain protein, is an upstream activator of the Rho GTPases Rac1, Cdc42, and RhoA. PMID: 15023524

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Subcellular Location
Cytoplasm. Cell membrane. Nucleus. Cytoplasm, cytoskeleton. Cytoplasm, perinuclear region. Cell projection, filopodium.
Tissue Specificity
Broadly expressed in the immune system and can be detected in T and B-cells.
Database Links

HGNC: 2760

OMIM: 610094

KEGG: hsa:50619

STRING: 9606.ENSP00000319831

UniGene: Hs.15476

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