DEF6 Antibody

Datasheet

Code	CSB-PA884467LA01HU
Size	US$166
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Image	Western Blot Positive WB detected in: Mouse liver tissue, Mouse kidney tissue All lanes: DEF6 antibody at 4μg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 74 kDa Observed band size: 74 kDa Immunofluorescent analysis of U251 cells using CSB-PA884467LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Product Details
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) DEF6 Polyclonal antibody

Uniprot No.

Q9H4E7

Target Names

DEF6

Alternative Names

DEF-6 antibody; Def6 antibody; DEFI6_HUMAN antibody; Differentially expressed in FDCP 6 homolog antibody; Differentially expressed in FDCP 6 homologue mouse antibody; IBP antibody; IRF4-binding protein antibody; Swap70 like adapter of T cells antibody

Raised in

Rabbit

Species Reactivity

Human, Mouse

Immunogen

Recombinant Human Differentially expressed in FDCP 6 homolog protein (20-114AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The DEF6 Antibody (Product code: CSB-PA884467LA01HU) is Non-conjugated. For DEF6 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA884467LB01HU	DEF6 Antibody, HRP conjugated	ELISA
FITC	CSB-PA884467LC01HU	DEF6 Antibody, FITC conjugated
Biotin	CSB-PA884467LD01HU	DEF6 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IF	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

Phosphatidylinositol 3,4,5-trisphosphate-dependent guanine nucleotide exchange factor (GEF) which plays a role in the activation of Rho GTPases RAC1, RhoA and CDC42. Can regulate cell morphology in cooperation with activated RAC1. Plays a role in Th2 (T helper cells) development and/or activation, perhaps by interfering with ZAP70 signaling.

Gene References into Functions

this study identifies Def6 as a novel inhibitor of osteoclastogenesis in physiological and inflammatory conditions PMID: 28314855
DEF6 expression may serve as an independent prognostic factor, and interacted positively with p16 toward high tumor stage and shorter survival. PMID: 27488395
IBP was expressed ectopically in someoral squamous cell carcinoma samples and correlated significantly with tumor size, differentiation, clinical stage and distant metastases PMID: 23088770
DEF6, a novel substrate for the Tec kinase ITK, contains a glutamine-rich aggregation-prone region and forms cytoplasmic granules that co-localize with P-bodies. PMID: 22829599
Molecular cloning of IBP, a guanine nucleotide exchange factor, which is highly expressed in the immune system. PMID: 12651066
IBP is a novel guanine nucleotide exchange factor and is recruited to the immunological synapse by T cell receptor engagement PMID: 12923183
DEF6 not only activates Rho-family GTPases but also cooperates with activated Rac1 to exert its cellular function PMID: 17121847
level of expression correlates with the degree of malignancy in breast carcinoma tissues PMID: 19393345
Overexpression of the Interferon regulatory factor 4-binding protein is associated with Colorectal Carcinoma. PMID: 19679060
DEF6, a novel PH-DH-like domain protein, is an upstream activator of the Rho GTPases Rac1, Cdc42, and RhoA. PMID: 15023524

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Subcellular Location

Cytoplasm. Cell membrane. Nucleus. Cytoplasm, cytoskeleton. Cytoplasm, perinuclear region. Cell projection, filopodium.

Tissue Specificity

Broadly expressed in the immune system and can be detected in T and B-cells.

Database Links

HGNC: 2760

OMIM: 610094

KEGG: hsa:50619

STRING: 9606.ENSP00000319831

UniGene: Hs.15476

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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