DEPP1 Antibody

Datasheet
Code CSB-PA865135LA01HU
See More Details Free Antibody trial simple
Size US$299
Uniprot No. Q9NTK1
Image
  • IHC image of CSB-PA865135LA01HU diluted at 1:400 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • Immunofluorescence staining of HepG2 cells with CSB-PA865135LA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Protocols ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Immunogen Recombinant Human Protein DEPP1 protein (57-174AA)
Raised in Rabbit
Species Reactivity Human
Tested Applications ELISA, IHC, IF; Recommended dilution: IHC:1:200-1:500, IF:1:50-1:200
Relevance May play a role in autophagy.
Form Liquid
Conjugate Non-conjugated
Storage Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Purification Method >95%, Protein G purified
Isotype IgG
Clonality Polyclonal
Alias Protein DEPP1 (Decidual protein induced by progesterone) (Fasting-induced gene protein) (FIG), DEPP1, C10orf10 DEPP FIG
Immunogen Species Human
Research Area Others
Target Names DEPP1
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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Function Acts as a critical modulator of FOXO3-induced autophagy via increased cellular ROS.
Subcellular Location Cytoplasm, Peroxisome, Mitochondrion
Tissue Specificity Expressed in various tissues, including pancreas, placenta, ovary, testis and kidney.
Database Links

HGNC: 23355

OMIM: 611309

KEGG: hsa:11067

STRING: 9606.ENSP00000298295

UniGene: Hs.93675

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