DHX33 Antibody

Datasheet

Code	CSB-PA884479LA01HU
Size	US$166
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Image	Western blot All lanes: DHX33 antibody at 16µg/ml Lane 1: Hela whole cell lysate Lane 2: 293T whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 79, 61 kDa Observed band size: 79 kDa Immunohistochemistry of paraffin-embedded human breast cancer using CSB-PA884479LA01HU at dilution of 1:100 Immunohistochemistry of paraffin-embedded human lung cancer using CSB-PA884479LA01HU at dilution of 1:100 Immunofluorescent analysis of Hela cells using CSB-PA884479LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Product Details
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) DHX33 Polyclonal antibody

Uniprot No.

Q9H6R0

Target Names

DHX33

Alternative Names

DHX33 antibody; DDX33ATP-dependent RNA helicase DHX33 antibody; EC 3.6.4.13 antibody; DEAH box protein 33 antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human ATP-dependent RNA helicase DHX33 protein (116-357AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The DHX33 Antibody (Product code: CSB-PA884479LA01HU) is Non-conjugated. For DHX33 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA884479LB01HU	DHX33 Antibody, HRP conjugated	ELISA
FITC	CSB-PA884479LC01HU	DHX33 Antibody, FITC conjugated
Biotin	CSB-PA884479LD01HU	DHX33 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:1000-1:5000
IHC	1:20-1:200
IF	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Customer Reviews and Q&A

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Target Background

Function

Implicated in nucleolar organization, ribosome biogenesis, protein synthesis and cytoplasmic dsRNA sensing. Stimulates RNA polymerase I transcription of the 47S precursor rRNA. Associates with ribosomal DNA (rDNA) loci where it is involved in POLR1A recruitment. In the cytoplasm, promotes elongation-competent 80S ribosome assembly at the late stage of mRNA translation initiation. Senses cytosolic dsRNA mediating NLRP3 inflammasome formation in macrophages and type I interferon production in myeloid dendritic cells. Required for NLRP3 activation induced by viral dsRNA and bacterial RNA. In dendritic cells, required for induction of type I interferon production induced by cytoplasmic dsRNA via the activation of MAPK and NF-kappa-B signaling pathways.

Gene References into Functions

DHX33 doublet is due to alternative translation initiation by two in-frame initiation codons. DHX33 translation initiation from either AUG codon happens at equal efficiency. Short DHX33 protein has similar cellular location and functions with full-length DHX33. Leaky scanning normally occurs in DHX33 mRNA translation, which may serve as a safeguard mechanism to ensure optimal DHX33 translation efficiency. PMID: 29864424
In clinical samples, the expression of Rab1A or DHX33 was reversely correlated with miR-634. Re-expression of Rab1A or DHX33 abrogated the miR-634-mediated inhibition of cell proliferation and migration. Collectively, our data suggest a tumor suppressor role of miR-634 in hepatocellular carcinoma. PMID: 27693040
High expression of DHX33 is associated with non-Hodgkin's lymphoma and acute myeloid leukemia. PMID: 28498893
Study demonstrates that DHX33 acts as a direct transcriptional regulator to promote cell cycle progression and plays an important role in driving cell proliferation during both embryo development and tumorigenesis. PMID: 27601587
Our data therefore suggest DHX33 is overexpressed in HCC and serves as a promising prognostic biomarker for this deadly disease. PMID: 27073163
The results reveal a newly recognized function of DHX33 in mRNA translation initiation, further solidifying its central role in promoting cell growth and proliferation. PMID: 26100019
Data indicate that DHX33 forms the inflammasome complex with NLRP3 following stimulation with cytosolic RNA. PMID: 23871209
results directly implicate DHX33 as a crucial player in establishing rRNA synthesis rates in the face of Ras(V12) or ARF signals, adjusting ribosome biogenesis to match the appropriate growth or antigrowth signals. PMID: 23401854
we show the mechanistic importance of DHX33 in rRNA transcription and proliferation PMID: 21930779

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Subcellular Location

Nucleus, nucleolus. Nucleus, nucleoplasm. Cytoplasm. Nucleus. Inflammasome.

Protein Families

DEAD box helicase family, DEAH subfamily

Database Links

HGNC: 16718

OMIM: 614405

KEGG: hsa:56919

STRING: 9606.ENSP00000225296

UniGene: Hs.250456

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Protein Service

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Related pathways

NOD-like receptor signaling pathway

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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