DHX34 Antibody

Datasheet

Code	CSB-PA621761LA01HU
Size	US$166
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Image	IHC image of CSB-PA621761LA01HU diluted at 1:500 and staining in paraffin-embedded human cervical cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. IHC image of CSB-PA621761LA01HU diluted at 1:500 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunofluorescence staining of A549 cells with CSB-PA621761LA01HU at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) DHX34 Polyclonal antibody

Uniprot No.

Q14147

Target Names

DHX34

Alternative Names

1200013B07Rik antibody; 1810012L18Rik antibody; Ddx34 antibody; DEAH (Asp-Glu-Ala-His) box polypeptide 34 antibody; DEAH box protein 34 antibody; Dhx34 antibody; DHX34_HUMAN antibody; EC 3.6.1.- antibody; HRH1 antibody; KIAA0134 antibody; mKIAA0134 antibody; Probable ATP-dependent RNA helicase DHX34 antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Probable ATP-dependent RNA helicase DHX34 protein (418-554AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The DHX34 Antibody (Product code: CSB-PA621761LA01HU) is Non-conjugated. For DHX34 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA621761LB01HU	DHX34 Antibody, HRP conjugated	ELISA
FITC	CSB-PA621761LC01HU	DHX34 Antibody, FITC conjugated
Biotin	CSB-PA621761LD01HU	DHX34 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, IHC, IF

Recommended Dilution

Application	Recommended Dilution
IHC	1:500-1:1000
IF	1:50-1:200

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

Probable ATP-binding RNA helicase.

Gene References into Functions

DHX34 activates nonsense-mediated mRNA decay by functioning as a scaffold for SMG1-mediated UPF1 phosphorylation via complex formation with SMG1 and UPF1. PMID: 26841701
DHX34 promotes mRNP remodeling and triggers the conversion from the SURF complex to the DECID complex resulting in NMD activation. PMID: 25220460
DHX34 and NBAS act in concert with core nonsense-mediated mRNA decay factors to co-regulate a large number of endogenous RNA targets. PMID: 23828042

Protein Families

DEAD box helicase family, DEAH subfamily

Tissue Specificity

Expressed in whole blood, testis and spleen. Also expressed in the brain.

Database Links

HGNC: 16719

OMIM: 615475

KEGG: hsa:9704

STRING: 9606.ENSP00000331907

UniGene: Hs.151706

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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