DNAJC15 Antibody

Datasheet

Code	CSB-PA22849A0Rb
Size	US$166
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Image	IHC image of CSB-PA22849A0Rb diluted at 1:300 and staining in paraffin-embedded human heart tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. IHC image of CSB-PA22849A0Rb diluted at 1:300 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) DNAJC15 Polyclonal antibody

Uniprot No.

Q9Y5T4

Target Names

DNAJC15

Alternative Names

Cell growth inhibiting gene 22 protein antibody; Cell growth-inhibiting gene 22 protein antibody; DJC15_HUMAN antibody; DnaJ (Hsp40) homolog subfamily C member 15 antibody; DNAJ domain containing antibody; DnaJ homolog subfamily C member 15 antibody; DNAJC15 antibody; DNAJD1 antibody; GIG22 antibody; HSD18 antibody; MCJ antibody; Methylation controlled J protein antibody; Methylation-controlled J protein antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human DnaJ homolog subfamily C member 15 protein (59-150AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The DNAJC15 Antibody (Product code: CSB-PA22849A0Rb) is Non-conjugated. For DNAJC15 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA22849B0Rb	DNAJC15 Antibody, HRP conjugated	ELISA
FITC	CSB-PA22849C0Rb	DNAJC15 Antibody, FITC conjugated
Biotin	CSB-PA22849D0Rb	DNAJC15 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, IHC

Recommended Dilution

Application	Recommended Dilution
IHC	1:200-1:500

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Customer Reviews and Q&A

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Target Background

Function

Negative regulator of the mitochondrial respiratory chain. Prevents mitochondrial hyperpolarization state and restricts mitochondrial generation of ATP. Acts as an import component of the TIM23 translocase complex. Stimulates the ATPase activity of HSPA9.

Gene References into Functions

DNAJC15 as a new target gene responsible for ETV7-mediated Doxorubicin-resistance. PMID: 30025229
Acetaminophen interferes with the formation of mitochondrial respiratory supercomplexes via the mitochondrial negative regulator MCJ. Study also show higher levels of MCJ in livers from patients with acetaminophen-induced liver injury. PMID: 29233977
this study shows that memory CD8+ T cells lacking MCJ provide superior protection against influenza virus infection PMID: 27234056
Both DnaJC15 and DnaJC19 formed two distinct subcomplexes with Magmas at the import channel. PMID: 27330077
These results identify the MCJ gene as a transcriptional target of IFNgamma and provide evidence of the dynamic adaptation of normal tissues to changes in the environment as a way to adapt metabolically to new conditions PMID: 26419808
Changes in the expression levels of IRS1, IRS2, RIPK2, RSPO1, and DNA JC15 genes might contribute to the development of insulin resistance and glucose intolerance in the obese boys. PMID: 26040030
Report functional connection between mitochondrial inner membrane protein translocation machinery-associated J-protein DnaJC15 and regulation of cell death pathways. PMID: 24603329
MCJ functions as J co-chaperone of the human TIM23 pre-protein translocase. PMID: 23263864
loss of expression of MCJ by DNA methylation is associated with drug-resistance in ovarian cancer PMID: 14729589
evidence of MCJ hypermethylation in intracranial primitive neuroectodermal tumours (PNETs) (medulloblastomas, supratentorial PNETs & ependymomas); data indicate epigenetic inactivation of MCJ may play a role in development of pediatric brain tumours PMID: 16049974
MCJ is required in these cells to prevent c-Jun-mediated expression of ABCB1 and maintain drug response. PMID: 17283040
Following transient expression of MDR-1 and MCJ, changes in the sensitivity of Sk-Ov-3 cells to paclitaxel were detected whereas expression of Src, Bcl-2 and Bcl-X(L) decreased the sensitivity of Sk-Ov-3 cells to carboplatin. PMID: 18324624

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Involvement in disease

Absent or down-regulated in many advanced cases of ovarian adenocarcinoma, due to hypermethylation and allelic loss. Loss of expression correlates with increased resistance to antineoplastic drugs, such as cisplatin.

Subcellular Location

Mitochondrion inner membrane; Single-pass membrane protein.

Tissue Specificity

Expressed at highest levels in heart, followed by liver and kidney.

Database Links

HGNC: 20325

OMIM: 615339

KEGG: hsa:29103

STRING: 9606.ENSP00000368523

UniGene: Hs.438830

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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