DNAJC3 Antibody

Datasheet

Code	CSB-PA615670LA01HU
Size	US$166
	Order now
Image	Immunohistochemistry of paraffin-embedded human ovarian cancer using CSB-PA615670LA01HU at dilution of 1:100 Immunohistochemistry of paraffin-embedded human melanoma using CSB-PA615670LA01HU at dilution of 1:100 Immunofluorescent analysis of Hela cells using CSB-PA615670LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
The Latest Promotion
Have Questions?	Leave a Message or Start an on-line Chat

Product Details
Related Products
Customer Reviews and Q&A
Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) DNAJC3 Polyclonal antibody

Uniprot No.

Q13217

Target Names

DNAJC3

Alternative Names

DnaJ (Hsp40) homolog subfamily C member 3 antibody; DnaJ homolog subfamily C member 3 antibody; DNAJC3 antibody; DNJC3_HUMAN antibody; double-stranded RNA-activated protein kinase inhibitor antibody; Endoplasmic reticulum DnaJ protein 6 antibody; ERdj6 antibody; HP58 antibody; Interferon induced; double stranded RNA activated protein kinase inhibitor antibody; Interferon-induced antibody; P58 antibody; p58 ipk antibody; P58IPK antibody; PRKRI antibody; Protein kinase inhibitor of 58 kDa antibody; Protein kinase inhibitor p58 antibody; Protein kinase interferon inducible double stranded RNA dependent inhibitor antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human DnaJ homolog subfamily C member 3 protein (277-496AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The DNAJC3 Antibody (Product code: CSB-PA615670LA01HU) is Non-conjugated. For DNAJC3 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA615670LB01HU	DNAJC3 Antibody, HRP conjugated	ELISA
FITC	CSB-PA615670LC01HU	DNAJC3 Antibody, FITC conjugated
Biotin	CSB-PA615670LD01HU	DNAJC3 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid

Tested Applications

ELISA, IHC, IF

Recommended Dilution

Application	Recommended Dilution
IHC	1:20-1:200
IF	1:50-1:200

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Customer Reviews and Q&A

■ Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Target Background

Function

Involved in the unfolded protein response (UPR) during endoplasmic reticulum (ER) stress. Acts as a negative regulator of the EIF2AK4/GCN2 kinase activity by preventing the phosphorylation of eIF-2-alpha at 'Ser-52' and hence attenuating general protein synthesis under ER stress, hypothermic and amino acid starving stress conditions. Co-chaperone of HSPA8/HSC70, it stimulates its ATPase activity. May inhibit both the autophosphorylation of EIF2AK2/PKR and the ability of EIF2AK2 to catalyze phosphorylation of the EIF2A. May inhibit EIF2AK3/PERK activity.

Gene References into Functions

DNAJC3 p.H238N is likely to be a variant causing diabetes. PMID: 29767246
p58IPK is a general inhibitor of the eIF2alpha kinases in that it also interacts with GCN2. Thus forced overexpression of cytoplasmic p58 delays eIF2alpha phosphorylation, suppresses GCN2 phosphorylation and prolongs protein synthesis PMID: 25329545
Loss-of-function DNAJC3 mutations lead to a monogenic, recessive form of diabetes mellitus in humans. PMID: 25466870
p58(IPK) suppresses coxsackievirus B3-induced apoptosis through selective activation of PI3K/Akt pathway that requires activation of ATF6a and subsequently upregulates mitofusin 2. PMID: 24134518
interplay between p38 phosphorylation and p58(IPK) upregulation has key roles in modulating ERp29-induced cell-growth arrest and survival PMID: 22064321
the crystal structure of human P58(IPK) PMID: 21799829
Coxsackievirus B3 infection induces apoptosis through downregulation of p58IPK and activation of CHOP and SREBP1. PMID: 20554776
Spleen cells from transgenic mice overexpressing (Hu)PKR were studied and compared to those from wild-type mice after drug treatment. Dact-treated spleen cells were resistant to apoptosis; ConA-treated cells had decreased cell division. PMID: 12034040
P58(IPK) is an important component of a negative feedback loop used by the cell to inhibit eIF2alpha signaling, and thus attenuate the unfolded protein response. PMID: 12601012
The study demonstrates that P58(IPK) inhibits endoplasmic reticulum (ER) stress and plays an important role in maintaining balance and stability of the ER in human retinal capillary endothelial cells. PMID: 18568130

Hide All

Involvement in disease

Ataxia, combined cerebellar and peripheral, with hearing loss and diabetes mellitus (ACPHD)

Subcellular Location

Endoplasmic reticulum.

Tissue Specificity

Widely expressed with high level in the pancreas and testis. Also expressed in cell lines with different levels.

Database Links

HGNC: 9439

OMIM: 601184

KEGG: hsa:5611

STRING: 9606.ENSP00000365991

UniGene: Hs.59214

Promotions

Transmembrane Protein Questionnaire with Gifts

Email: [email protected]
Distributors Worldwide

Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Related pathways

Protein processing in endoplasmic reticulum

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

Join us with

Subscribe newsletter

Leave a message

Message

Product >> Product Name：
Product >> Code：
Name： *
Email： *
Company/Organization：
Phone：
Since emails may get flagged by spam filters occasionally, we recommend leaving your phone number as a precaution. Your information is safe with us.
Question/Comment： *
Your Region： *
I'd like to receive email newsletters from Cusabio

Yes

No
How do you know CUSABIO?

Email

Distributor

Search engine

Advertisement

Others
CAPTCHA verification：

Place an order now

I. Product details

Product name *

Code *

Size *

Quantity *

Message

II. Contact details

Name *

Email address *

Company/Organization

Phone

III. Ship To

Address *

City *

State/Province *

Zip/Post code *

Country *

Attn Name *

Phone# *

IV. Bill To Same as Shipping Address