EEF2KMT Antibody

Datasheet

Code	CSB-PA853420LA01HU
Size	US$166
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Image	Western Blot Positive WB detected in: Hela whole cell lysate(20µg), HepG2 whole cell lysate(20µg), 293Twhole cell lysate(20µg), THP-1 whole cell lysate(20µg), MCF7 whole cell lysate(20µg), A431 whole cell lysate(20µg) All lanes: EEF2KMT antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/20000 dilution Predicted band size: 37, 34 kDa Observed band size: 37 kDa Exposure time: 60s IHC image of CSB-PA853420LA01HU diluted at 1:100 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody IHC image of CSB-PA853420LA01HU diluted at 1:100 and staining in paraffin-embedded human cervical cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody
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Product Details
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) EEF2KMT Polyclonal antibody

Uniprot No.

Q96G04

Target Names

EEF2KMT

Alternative Names

EEF2KMT antibody; FAM86A antibody; SB153Protein-lysine N-methyltransferase EEF2KMT antibody; EC 2.1.1.- antibody; eEF2-lysine methyltransferase antibody; eEF2-KMT antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Synthesized peptide derived from human FA86A

Immunogen Species

Homo sapiens (Human)

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen affinity purification

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Form

Liquid

Tested Applications

ELISA, WB, IHC

Recommended Dilution

Application	Recommended Dilution
WB	1:1000-1:3000
IHC	1:100-1:300

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

Catalyzes the trimethylation of eukaryotic elongation factor 2 (EEF2) on 'Lys-525'.

Gene References into Functions

Previously uncharacterized lysine-specific methyltransferase FAM86A and Yjr129c introduce a functionally important lysine methylation in eEF2. PMID: 25231979

Subcellular Location

Cytoplasm.

Protein Families

Class I-like SAM-binding methyltransferase superfamily, EEF2KMT family

Database Links

HGNC: 32221

OMIM: 615263

KEGG: hsa:196483

STRING: 9606.ENSP00000398502

UniGene: Hs.406461

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Service

Phage Display Service

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Protein Service

Gene Synthesis Service

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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