EHD2 Antibody

Datasheet

Citations (2)

Code	CSB-PA873710LA01HU
Size	US$166
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Image	Western Blot Positive WB detected in: Hela whole cell lysate, Mouse liver tissue, Mouse kidney tissue All lanes: EHD2 antibody at 3μg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 62, 47 kDa Observed band size: 62, 47 kDa Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA873710LA01HU at dilution of 1:100 Immunofluorescent analysis of Hela cells using CSB-PA873710LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Product Details
Citations
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Customer Reviews and Q&A
Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) EHD2 Polyclonal antibody

Uniprot No.

Q9NZN4

Target Names

EHD2

Alternative Names

EH domain containing 2 antibody; EH domain containing 3 antibody; EH domain containing protein 2 antibody; EH domain-containing protein 2 antibody; EHD 2 antibody; Ehd2 antibody; EHD2_HUMAN antibody; EHD3 antibody; FLJ96617 antibody; PAST 2 antibody; PAST homolog 2 antibody; PAST2 antibody

Raised in

Rabbit

Species Reactivity

Human, Mouse

Immunogen

Recombinant Human EH domain-containing protein 2 protein (401-543AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The EHD2 Antibody (Product code: CSB-PA873710LA01HU) is Non-conjugated. For EHD2 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA873710LB01HU	EHD2 Antibody, HRP conjugated	ELISA
FITC	CSB-PA873710LC01HU	EHD2 Antibody, FITC conjugated
Biotin	CSB-PA873710LD01HU	EHD2 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:20-1:200
IF	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

The EHD2 antibody is prepared by immunizing rabbits with the recombinant human EHD2 protein (401-543AA) and by purifying rabbit antiserum by protein G. It is a polyclonal antibody and exists an unconjugated IgG. Protein G purified EHD2 antibody reaches up to 95% in purity. It is reactive with human and mouse EHD2 protein. The target protein EHD2 is a dynamin-related ATPase located at the neck of caveolae and negatively regulates caveolae-dependent lipid uptake and plays a role in caveolar stability and dynamics for lipid homeostasis and obesity. This EHD2 antibody is suitable for the detection of ELISA, WB, IF, and IHC applications.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Citations

Plasma membrane curvature regulates the formation of contacts with the endoplasmic reticulum Y Yang, LA Valencia, CH Lu, ML Nakamoto,Nature Cell Biology,2024
Membrane Curvature Promotes ER-PM Contact Formation via Junctophilin-EHD Interactions Y Yang,bioRxiv,2024

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Target Background

Function

ATP- and membrane-binding protein that controls membrane reorganization/tubulation upon ATP hydrolysis. Plays a role in membrane trafficking between the plasma membrane and endosomes. Important for the internalization of GLUT4. Required for fusion of myoblasts to skeletal muscle myotubes. Required for normal translocation of FER1L5 to the plasma membrane. Regulates the equilibrium between cell surface-associated and cell surface-dissociated caveolae by constraining caveolae at the cell membrane.

Gene References into Functions

Our prognostic model is useful for predicting persistent/recurrent disease after surgery of Papillary thyroid carcinoma (PTC). EHD2 mRNA expression could be a novel prognostic marker for PTC patients. PMID: 28358874
EHD2 expression, along with the epithelial marker E-cadherin, was markedly reduced in tumor tissues than in adjacent noncancerous tissues. Molecular data indicated that EHD2 inhibited migration and invasion of hepatocellular carcinoma probably by interacting with E-cadherin. PMID: 27221498
Data suggest that the EH-domain containing 2 protein (EHD2) NPF phenylalanine residue is crucial for EHD2 localization to the plasma membrane, whereas the proline residue is essential for EHD2 dimerization and binding. PMID: 25875965
EHD2 can inhibit the metastasis of human breast cancer by regulating the epithelial-to-mesenchymal transition markers E-cadherin and N-cadherin. PMID: 25758127
Downregulation of EHD2 was associated with migration and invasion by abrogating the expression of Rac1 in breast cancer patients. PMID: 25557791
Phosphatidylinositol 4,5-bisphosphate controls EHD2 plasma membrane localization. PMID: 24040268
Our results suggested that EHD2 low expression is involved in the pathogenesis of human esophageal squamous cell carcinoma PMID: 23354948
EHD2 participates in the sarcolemma repair. PMID: 22679923
Assembly of EHD2 stabilized and constrained caveolae to the plasma membrane to control turnover, and depletion of EHD2, resulting in endocytic and more dynamic and short-lived caveolae. PMID: 22323287
Among three EHD proteins (EHD1-EHD3) that were tested, only EHD2 accumulates in the nucleus under nuclear export inhibition treatment. PMID: 22448906
Confining caveolae to the plasma membrane by EHD2 relied on its capacity to link caveolae to actin filaments. PMID: 22505029
EHD2 regulates trafficking from the plasma membrane by controlling Rac1 activity. PMID: 21756249

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Subcellular Location

Cell membrane; Peripheral membrane protein; Cytoplasmic side. Membrane, caveola; Peripheral membrane protein; Cytoplasmic side. Endosome membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasm, cytosol.

Protein Families

TRAFAC class dynamin-like GTPase superfamily, Dynamin/Fzo/YdjA family, EHD subfamily

Tissue Specificity

Highly expressed in heart and moderately expressed in placenta, lung, and skeletal muscle.

Database Links

HGNC: 3243

OMIM: 605890

KEGG: hsa:30846

STRING: 9606.ENSP00000263277

UniGene: Hs.744963

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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