EIF1 Antibody

Code CSB-PA007503LA01HU
Size US$166
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Image
  • Immunofluorescence staining of A549 cells with CSB-PA007503LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Product Details

Full Product Name
Rabbit anti-Homo sapiens (Human) EIF1 Polyclonal antibody
Uniprot No.
Target Names
EIF1
Alternative Names
EIF1 antibody; SUI1Eukaryotic translation initiation factor 1 antibody; eIF1 antibody; A121 antibody; Protein translation factor SUI1 homolog antibody; Sui1iso1 antibody
Raised in
Rabbit
Species Reactivity
Human
Immunogen
Recombinant Human Eukaryotic translation initiation factor 1 protein (2-113AA)
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated

The EIF1 Antibody (Product code: CSB-PA007503LA01HU) is Non-conjugated. For EIF1 Antibody with conjugates, please check the following table.

Available Conjugates
Conjugate Product Code Product Name Application
HRP CSB-PA007503LB01HU EIF1 Antibody, HRP conjugated ELISA
FITC CSB-PA007503LC01HU EIF1 Antibody, FITC conjugated
Biotin CSB-PA007503LD01HU EIF1 Antibody, Biotin conjugated ELISA
Clonality
Polyclonal
Isotype
IgG
Purification Method
>95%, Protein G purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, IF
Recommended Dilution
Application Recommended Dilution
IF 1:50-1:200
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Background

Function
Necessary for scanning and involved in initiation site selection. Promotes the assembly of 48S ribosomal complexes at the authentic initiation codon of a conventional capped mRNA.
Gene References into Functions
  1. Anti-Sui1 could be utilized as a supplementary serological marker. PMID: 26386724
  2. eIF1A is a novel component of the Ago2-centred RNA-induced silencing complexes (RISCs) and augments Ago2-dependent RNAi and miRNA biogenesis. PMID: 26018492
  3. eEF4GI shares this activity and also interacts with eIF1. PMID: 25738462
  4. eIF1, eIF1A, eIF3j, and the eIF2-GTP-Met-tRNAi ternary complex stably bind to the 43 S preinitiation complex PMID: 25246524
  5. Coordinated movements of eukaryotic translation initiation factors eIF1, eIF1A, and eIF5 trigger phosphate release from eIF2 in response to start codon recognition by the ribosomal preinitiation complex PMID: 23293029
  6. This study provides mechanistic insight into the role of eIF5-carboxyl terminal domain's dynamic interplay with eIF1 and eIF2beta. PMID: 22813744
  7. show that the stringency of start codon selection (preferential utilization of optimal start sites) is increased to a surprising degree by overexpressing eIF1 PMID: 20921384

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Protein Families
SUI1 family
Database Links

HGNC: 3249

KEGG: hsa:10209

STRING: 9606.ENSP00000419449

UniGene: Hs.150580

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