EIF3H Antibody

Datasheet

Code	CSB-PA903985
Size	US$166
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Image	The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using CSB-PA903985(EIF3H Antibody) at dilution 1/50, on the right is treated with fusion protein. (Original magnification: ×200) The image on the left is immunohistochemistry of paraffin-embedded Human brain tissue using CSB-PA903985(EIF3H Antibody) at dilution 1/50, on the right is treated with fusion protein. (Original magnification: ×200) Gel: 8%SDS-PAGE, Lysate: 40 μg, Lane 1-2: Human adrenal pheochromocytoma tissue, Human fetal brain tissue, Primary antibody: CSB-PA903985(EIF3H Antibody) at dilution 1/1150, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 40 seconds
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Product Details
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Target Background

Product Details

Uniprot No.

O15372

Target Names

EIF3H

Alternative Names

eIF-3-gamma antibody; eIF3 gamma antibody; eIF3 p40 antibody; eIF3 p40 subunit antibody; eIF3h antibody; EIF3H_HUMAN antibody; EIF3S3 antibody; Eukaryotic translation initiation factor 3 subunit 3 antibody; Eukaryotic translation initiation factor 3 subunit H antibody; MGC102958 antibody

Raised in

Rabbit

Species Reactivity

Human,Mouse,Rat

Immunogen

Fusion protein of Human EIF3H

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Isotype

IgG

Purification Method

Antigen affinity purification

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

-20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol

Form

Liquid

Tested Applications

ELISA,WB,IHC

Recommended Dilution

Application	Recommended Dilution
ELISA	1:2000-1:5000
WB	1:500-1:2000
IHC	1:100-1:300

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression.

Gene References into Functions

EIF3H knockdown inhibited cell proliferation and colony formation in gastric cancer lines and led to cell cycle arrest at the G0/G1 phase, while inducing apoptosis via up- and downregulation of pro- and anti-apoptotic factors, respectively. These results indicate that EIF3H can serve as a novel therapeutic target for the clinical treatment of gastric cancer. PMID: 30060823
EIF3H plays key roles in the apoptosis in colorectal cancer cells, which suggests EIF3H as a potential diagnostic biomarker in colorectal cancer PMID: 30022709
eukaryotic translation initiation factor 3H has roles in proliferation, invasion and tumorigenicity in human hepatocellular carcinoma PMID: 27340783
-RAD21 and EIF3H, both on chromosome 8q23, CHRAC1 on chromosome 8q24.3 and TANC2 on chromosome 17q23-were confirmed to be driver genes regulating the proliferation/survival of clonogenic breast cancer cells PMID: 24148822
Increased expression of EIF3H gene increases colorectal cancer growth and invasiveness thereby providing a biological mechanism for the 8q23.3 association. PMID: 20862326
Gain of EIF3S3 was associated with poor cancer-specific survival in incidentally found prostate carcinomas. PMID: 11733359
PTK2 and EIF3S3, which, respectively, encode focal adhesion kinase and the p40 subunit of the eukaryotic initiation factor 3, were probable targets within the amplification at 8q23-q24 and may be involved in progression of HCC. PMID: 14578863
overexpression of EIF3S3 is associated with breast and prostate cancer PMID: 14997205
results suggest that eukaryotic translation initiation factor 3, subunit 3 (EIF3S3) regulates cell growth and viability, and that overexpression of the gene may provide growth advantage to the cancer cells PMID: 16652384
high eIF3h levels directly stimulate protein synthesis, resulting in the establishment and maintenance of the malignant state in cells. PMID: 18544531
MYC and EIF3H are frequently coamplified in NSCLC and that a high copy number correlates with increased epidermal growth factor receptor tyrosine kinase inhibitors sensitivity. PMID: 19204574

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Subcellular Location

Cytoplasm.

Protein Families

EIF-3 subunit H family

Database Links

HGNC: 3273

OMIM: 603912

KEGG: hsa:8667

STRING: 9606.ENSP00000429931

UniGene: Hs.492599

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Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Related pathways

Target Therapy

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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