EIF5 Antibody

Datasheet

Code	CSB-PA007572HA01HU
Size	US$166
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Image	Immunohistochemistry of paraffin-embedded human pancreatic cancer using CSB-PA007572HA01HU at dilution of 1:100 Immunofluorescent analysis of PC-3 cells using CSB-PA007572HA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Product Details
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) EIF5 Polyclonal antibody

Uniprot No.

P55010

Target Names

EIF5

Alternative Names

2810011H21Rik antibody; D12Ertd549e antibody; EIF 5 antibody; EIF 5A antibody; eIF-5 antibody; Eif5 antibody; Eukaryotic initiation factor 5 antibody; Eukaryotic translation initiation factor 5 antibody; IF5_HUMAN antibody; MGC36374 antibody; MGC36509 antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Eukaryotic translation initiation factor 5 protein (1-431AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The EIF5 Antibody (Product code: CSB-PA007572HA01HU) is Non-conjugated. For EIF5 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA007572HB01HU	EIF5 Antibody, HRP conjugated	ELISA
FITC	CSB-PA007572HC01HU	EIF5 Antibody, FITC conjugated
Biotin	CSB-PA007572HD01HU	EIF5 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid

Tested Applications

ELISA, IHC, IF

Recommended Dilution

Application	Recommended Dilution
IHC	1:20-1:200
IF	1:50-1:200

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

Catalyzes the hydrolysis of GTP bound to the 40S ribosomal initiation complex (40S.mRNA.Met-tRNA[F].eIF-2.GTP) with the subsequent joining of a 60S ribosomal subunit resulting in the release of eIF-2 and the guanine nucleotide. The subsequent joining of a 60S ribosomal subunit results in the formation of a functional 80S initiation complex (80S.mRNA.Met-tRNA[F]).

Gene References into Functions

The down-regulation of the GCN4 expression (Gcn(-) phenotype) in the eIF5(G31R) mutant was not because of leaky scanning defects; rather was due to the utilization of upUUG initiation codons at the 5' regulatory region present between uORF1 and the main GCN4 ORF. PMID: 28385532
overexpression of eIF5 and 5MP induces translation of ATF4 PMID: 27325740
it is eIF5-induced GTP hydrolysis and Pi release that irreversibly trap the 48S complex, and this complex is further stabilized by eIF5B and 60S joining. PMID: 26717981
The N-terminal tail of eIF1A mediates the interaction with eIF5 and eIF1. PMID: 24319994
This study provides mechanistic insight into the role of eIF5-carboxyl terminal domain's dynamic interplay with eIF1 and eIF2beta. PMID: 22813744
miR-5787 represses cell growth, in part, by targeting eIF5. PMID: 22062548
3-dimensional solution structure of N-terminal domain of human eIF5, has 2 subdomains, both reminiscent of nucleic-acid-binding modules. N-terminal subdomain contains "arginine finger" motif essential for GAP function. PMID: 16584190
The carboxy-terminal domain (CTD)of eIF5 is exclusively composed out of alpha-helices and is homologous to the carboxy-terminal domain of eIF2B-epsilon (eIF2Bepsilon-CTD). The binding sites of eIF2-beta, eIF3 and eIF1 were mapped onto the structure. PMID: 16781736

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Protein Families

EIF-2-beta/eIF-5 family

Database Links

HGNC: 3299

OMIM: 601710

KEGG: hsa:1983

STRING: 9606.ENSP00000216554

UniGene: Hs.433702

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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