EMC8 Antibody

Datasheet

Code	CSB-PA005835LA01HU
Size	US$166
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Image	Immunofluorescence staining of MCF-7 cells with CSB-PA005835LA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Western blot All lanes: EMC8 antibody at 4µg/ml Lane 1: A549 whole cell lysate Lane 2: Human placenta tissue Lane 3: Hela whole cell lysate Lane 4: HT29 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 24, 14 kDa Observed band size: 24 kDa Immunohistochemistry of paraffin-embedded human cervical cancer using CSB-PA005835LA01HU at dilution of 1:100 Immunohistochemistry of paraffin-embedded human adrenal gland tissue using CSB-PA005835LA01HU at dilution of 1:100
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Product Details
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) EMC8 Polyclonal antibody

Uniprot No.

O43402

Target Names

EMC8

Alternative Names

C16orf2 antibody; C16orf4 antibody; COX4 neighbor antibody; COX4; neighbor of antibody; COX4AL antibody; Cox4nb antibody; CX4NB_HUMAN antibody; ER membrane protein complex subunit 8 antibody; FAM158B antibody; family with sequence similarity 158; member B antibody; Neighbor of COX4 antibody; NOC4 antibody; Protein FAM158B antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human ER membrane protein complex subunit 8 protein (1-210AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The EMC8 Antibody (Product code: CSB-PA005835LA01HU) is Non-conjugated. For EMC8 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA005835LB01HU	EMC8 Antibody, HRP conjugated	ELISA
FITC	CSB-PA005835LC01HU	EMC8 Antibody, FITC conjugated
Biotin	CSB-PA005835LD01HU	EMC8 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:20-1:200
IF	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Customer Reviews and Q&A

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Target Background

Function

Part of the endoplasmic reticulum membrane protein complex (EMC) that enables the energy-independent insertion into endoplasmic reticulum membranes of newly synthesized membrane proteins. Preferentially accommodates proteins with transmembrane domains that are weakly hydrophobic or contain destabilizing features such as charged and aromatic residues. Involved in the cotranslational insertion of multi-pass membrane proteins in which stop-transfer membrane-anchor sequences become ER membrane spanning helices. It is also required for the post-translational insertion of tail-anchored/TA proteins in endoplasmic reticulum membranes. By mediating the proper cotranslational insertion of N-terminal transmembrane domains in an N-exo topology, with translocated N-terminus in the lumen of the ER, controls the topology of multi-pass membrane proteins like the G protein-coupled receptors. By regulating the insertion of various proteins in membranes, it is indirectly involved in many cellular processes (Probable).

Subcellular Location

Endoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side.

Protein Families

EMC8/EMC9 family

Tissue Specificity

Expressed in liver, pancreas, heart, lung, kidney, brain, skeletal muscle, and placenta. Expression levels are highest in pancreas and moderate in heart, skeletal muscle, and placenta.

Database Links

HGNC: 7864

OMIM: 604886

KEGG: hsa:10328

STRING: 9606.ENSP00000253457

UniGene: Hs.173162

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Service

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Protein Service

Gene Synthesis Service

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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