EP300/CREBBP Antibody

Datasheet
Code CSB-PA265859
Product Type Polyclonal Antibody
Size US$297
Uniprot No. Q09472
Image
  • Western blot analysis of extracts from COLO205 cells, using P300/CBP antibody.
  • Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using P300/CBP antibody.
Immunogen Synthesized peptide derived from Human P300/CBP.
Raised in Rabbit
Species Reactivity Human,Mouse,Rat
Specificity The antibody detects endogenous levels of total P300/CBP protein.
Tested Applications ELISA,WB,IHC;WB:1:500-1:3000,IHC:1:50-1:100
Relevance Acetylates histones, giving a specific tag for transcriptional activation. Also acetylates non-histone proteins, like NCOA3 and FOXO1. Binds specifically to phosphorylated CREB and enhances its transcriptional activity toward cAMP-responsive genes. Acts as a coactivator of ALX1 in the presence of EP300. Acts as a circadian transcriptional coactivator which enhances the activity of the circadian transcriptional activators: NPAS2-ARNTL/BMAL1 and CLOCK-ARNTL/BMAL1 heterodimers.

Gerd A. Blobel, J. Leukoc. Biol., Apr 2002; 71: 545.
Eric Kalkhoven, Hum. Mol. Genet., Feb 2003; 12: 441 - 450.
Wei-fang Shen, Mol. Cell. Biol., Nov 2001; 21: 7509 - 7522.
Frederick C. Nucifora, Science, Mar 2001; 291: 2423.

Form Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purification Method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Clonality Polyclonal
Alias CREB-binding protein; CREBBP E1A-associated protein p300; EP300; P300 transcription coactivator homolog;
Immunogen Species Homo sapiens (Human)
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Target Names EP300/CREBBP
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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Function Functions as histone acetyltransferase and regulates transcription via chromatin remodeling
Involvement in disease Rubinstein-Taybi syndrome 2 (RSTS2)
Subcellular Location Cytoplasm, Nucleus, Chromosome
Database Links

HGNC: 3373

OMIM: 602700

KEGG: hsa:2033

STRING: 9606.ENSP00000263253

UniGene: Hs.517517

Pathway cAMP signaling pathway
HIF-1 signaling pathway
Jak-STAT signaling pathway
Melanogenesis
Notch signaling pathway
TGF-beta signaling pathway
Wnt signaling pathway
Cell cycle
FoxO signaling pathway
Glucagon signaling pathway

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